Wheat flour with a crude protein content of 12.2% (N × 6.25) was purchased from Yihai Kerry Grain Industry Co., Ltd. (Shanghai, China). Fresh okara with a crude protein content of 19.35 ± 0.56% (N × 6.25) was obtained from the Beverage and Food Management Center at Yangzhou University (Yangzhou, China). The okara was dried in an oven (DHG‐9148A, Shanghai Jinghong Experimental Equipment Co., Ltd., China) at 60°C for 6 h. The dried okara was then ground using a grinder (H7d, Xiamen Hehui Electronic Technology Co., Fujian, China) and sieved through an 80‐mesh screen. 1,1‐Diphenyl‐2‐picrylhydrazyl, α‐amylase, and diammonium 2,2′‐azinobis (3‐ethylbenzothiazoline‐6‐sulfonate) were purchased from Shanghai Yuan Ye Biotechnology Co., Ltd. (Shanghai, China), and starch glucosidase was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). All chemicals used were of analytical grade.
Diammonium 2 2 azinobis 3 ethylbenzothiazoline 6 sulfonate
Manufactured by Yuanye Bio-Technology
Sourced in China
Diammonium 2,2′‐azinobis (3‐ethylbenzothiazoline‐6‐sulfonate) is a chemical compound used as a reagent in various laboratory and scientific applications. It serves as a redox indicator, undergoing color changes in response to oxidation-reduction reactions. The compound is soluble in water and commonly utilized in colorimetric assays and analytical procedures.
Automatically generated - may contain errors
2 protocols using diammonium 2 2 azinobis 3 ethylbenzothiazoline 6 sulfonate
1
Optimizing Okara Incorporation in Wheat Flour
2
Silk Fabric Functionalization with Laccase
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Silk fabric (72 g m−2) was provided by Jiangsu Huajia Group Co., Ltd., dopamine hydrochloride (AR) and tris(hydroxymethyl)aminomethane (AR) were purchased from SuZhou KeChuang Biotechnology Co., Ltd., laccase (from coriolus versicolor) and diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS, 98.0%) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd., CuSO4·5H2O (AR), AgNO3 (AR), FeCl3·6H2O (AR) and Ni(CH3COO)2·4H2O (AR), acetic acid (AR) and sodium acetate (AR) were purchased from Sinopharm Chemical Reagent Co., Ltd. The fabric was washed with soap flakes at 60 °C and rinsed before use, and the other chemical reagents were used as they were received. The activity of laccase needed to be measured before the experiment, and the test method was consistent with the previous method used by our group.22 (link) In brief, ABTS was used to determine the activity of laccase by an oxidation method at 415 nm (ε = 29 300 M−1 cm−1), and one laccase activity unit was defined as the amount of enzyme that transforms 1 μmol min−1 of ABTS at 25 °C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!