For the analysis of individual tubercles, photographs were taken with a Nikon Stereomicroscope Model SMZ1500 (Nikon, Tokio, Japan) equipped with a 5.24 megapixel Nikon DS-Fi1 of camera (Nikon, Tokio, Japan); lateral and dorsal views used in FET analysis were taken with a Nikon Z6 camera (Nikon, Tokio, Japan), equipped with an objective AF-S Micro NIKKOR 60 mm f/2.8G ED (Nikon, Tokio, Japan).
Af s micro nikkor 60 mm f 2.8g ed
Manufactured by Nikon
Sourced in Japan
The AF-S Micro NIKKOR 60 mm f/2.8G ED is a close-up photography lens designed for Nikon cameras. It features a focal length of 60 mm and a maximum aperture of f/2.8. The lens utilizes Nikon's Silent Wave Motor (SWM) for fast and quiet autofocus, and has an Extra-Low Dispersion (ED) glass element to minimize chromatic aberration.
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3 protocols using af s micro nikkor 60 mm f 2.8g ed
1
Tubercle Analysis Using Microscopy
2
Seed Image Acquisition and Analysis Protocol
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Photographs were taken with a Nikon Z6 camera with an objective AF-S Micro NIKKOR 60 mm f/2.8G ED. From an initial photograph made to the pool of seeds in each population and containing between 30 and 110 seeds, composed images containing 20–30 seeds regularly oriented per accession were prepared with Corel Photo Paint. In these images, the seeds were aligned to allow further analysis in ImageJ. The images are stored at: https://zenodo.org/record/6205212#.YhOpUujMKM8 (accessed on 21 February 2022).
3
High-Speed Imaging of Spinal Contusion
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The contusion sequences were filmed with a high-speed camera equipped with a macro lens (AF-S Micro Nikkor 60mm F/2.8G ED, Nikon, Japan). The images were taken from the lateral position, so that the exposed dura, the tip of the impactor, the dorsal parts of the C3 and C5 vertebrae and the two clamps were in the field of view (Figure 1C). Two different high-speed cameras were used during the study: the X-VIT LShigh-speed camera (AOS Technologies, Switzerland) at 1000 frames per second (fps) mode for three mice, and the FastCam SA3 (Photron USA, Inc.) at 3000 fps for three other mice.
By using the latter equipment, brighter images were obtained at a higher frequency were obtained without modifying the experimental design. The impact of this equipment change on the results accuracy has been assessed (see Results section).
By using the latter equipment, brighter images were obtained at a higher frequency were obtained without modifying the experimental design. The impact of this equipment change on the results accuracy has been assessed (see Results section).
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