Las af sp8

Manufactured by Leica

The LAS AF SP8 software is a comprehensive imaging solution designed for users of Leica's SP8 confocal microscope. It provides advanced tools for acquisition, processing, and analysis of high-quality images and data.

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Lab products found in correlation

Tcs sp8 aobs confocal laser scanning microscope, by Leica (2 mentions) Zen 2, by Zeiss (1 mentions) Elyra ps 1 microscope, by Zeiss (1 mentions)

Close spelling variants of this product

Leica Microsystems LAS AF TCS SP8 software LAS AF LAS SP8

2 protocols using las af sp8

Advanced Microscopy Techniques for Imaging

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Confocal images were acquired on a Leica TCS SP8 AOBS Confocal Laser Scanning Microscope (×63/1.4 NA objective for GUVs and fixed cells and ×63/1.2 NA objective for live cell imaging) using the Leica LAS AF SP8 software. Structured-Illumination Microscopy (SIM) was performed on a Zeiss Elyra PS.1 microscope (×63/1.4 NA objective) using the Zeiss ZEN 2 software with SR-SIM module.

Wetzel L., Blanchard S., Rama S., Beier V., Kaufmann A, & Wollert T. (2020). TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes. Nature Communications, 11, 2993.

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Confocal Imaging of GUVs and Cells

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Confocal images of GUVs and xed cells were acquired on a Leica TCS SP8 AOBS Confocal Laser Scanning Microscope (×63/1.4 NA objective) using the Leica LAS AF SP8 software. Atto405, GFP/Alexa 488 , rhodamine/mCherry/RFP/Alexa 561 , and Alexa 633 dyes were excited with 405 nm, 488 nm, 561 nm and 633 nm laser light, respectively. Multi-tracking was used for image acquisition and pinholes were set for acquisition of 0.5-1.0 µm optical slices. Images were analyzed using Fiji.

Wollert T., Blanc C., Moparthi S.B., Nugues C., Rama S., Vassilopoulos S., & Vassilopoulos S. (2022). Phagophore formation by the autophagy conjugation machinery.

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