Primary rat tenocytes, isolated as above, were seeded as described for the qualitative cytocompatibility assay for a period of 1, 7 and 14 days. At each time point, viability was assessed, as previously described [24 (link)]. Briefly, 50 μl alamarBlue® (×100, Invitrogen) (5 % final concentration in well) was added to each well for 4 h at 37 °C. Following this, 200 μl of the alamarBlue® conditioned medium was transferred to a 96-well plate (Greiner Bio-One) and fluorescence read using a Synergy 2 multi-detection microplate reader (BioTek Instruments Inc., USA).
Alamarblue
Manufactured by Greiner
Sourced in United States
AlamarBlue is a viability and cytotoxicity assay reagent. It is a non-toxic, water-soluble compound that changes color in response to chemical reduction, indicating metabolic activity in living cells.
Automatically generated - may contain errors
2 protocols using alamarblue
1
Rat Tenocyte Viability Assay
2
Osteoblast Proliferation on Hydrogels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Primary rat and mouse osteoblasts were seeded onto hydrogels (250 μL per well) pre-soaked in culture media, with or without LF. At each time point of day 1, 3 and 7, 5% alamarBlue® (Invitrogen) (final concentration in well) was added for 4 h at 37 °C. At the end of this incubation, 200 μL of the alamarBlue®-conditioned medium was transferred from each well to a 96-well plate (Greiner Bio-One) and fluorescence (excitation 540 nm; emission 630) read using a Synergy 2 multidetection microplate reader (BioTek Instruments, Inc.). For analysis, the results were normalised to the fluorescence readings from blank gels without cells (negative control).
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