Dinitrophenyl dnp ige

ME was purchased from the National Institute for Food and Drug Control (Beijing, China; purity, ≥99.5%). Dulbecco's minimum essential medium (DMEM), penicillin, streptomycin, and fetal bovine serum (FBS) were purchased from GIBCO (Grand Island, NY, USA). 4-[3-(4-Iodophenyl)-2-4(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1) was obtained from Dojindo (Kumamoto, Japan). Specific antibodies against phospho-Lyn, Lyn, phospho-Syk, Syk, phospho-ERK1/2, ERK1/2, phospho-p38, p38, phospho-JNK, JNK, cytosolic phospholipase A₂ (cPLA₂), phospho-cPLA₂, cyclooxygenase-2 (COX-2), and β-actin were purchased from Cell Signaling Technology (Beverly, MA, USA). Specific antibodies against phospho-5-lipoxygenase (5-LO) and 5-LO, and enzyme immunoassay (EIA) kits for prostaglandin E₂ (PGE₂), prostaglandin D₂ (PGD₂), leukotriene B₄ (LTB₄), and leukotriene C₄ (LTC₄) were purchased from Cayman Chemical (Ann Arbor, MI, USA). The enzyme-linked immunosorbent assay (ELISA) kits for TNF-α and IL-4 were obtained from Bangyi Technologies Inc. (Shanghai, China). Dinitrophenyl- (DNP-) IgE was obtained from Sigma-Aldrich (St Louis, MO, USA), and DNP-bovine serum albumin (BSA) was obtained from Biosearch Technologies Inc. (Novato, CA, USA). All other chemicals were of analytical grade and were purchased from Sigma-Aldrich.