Ve cadherin clone 55 7h1

Antibodies used were directed against β-actin (clone C4; Chemicon), integrin β3 (clone C-17 from Dr. E. van der Schoot, Sanquin Research, Amsterdam, The Netherlands; integrin β1 (clone TS2/16, Developmental Studies Hybridoma Bank), kindlin-2 (from Dr. R. Faessler, Max Planck Institute of Biochemistry, Martinsried, Germany), P(Y) (clone 4G10 from Sigma-Aldrich), Rac (Transduction laboratories), RhoA (Cell Signaling), VE-Cadherin (clone 55-7H1, BD biosciences) and VEGFR2 (R&D Systems). The β3 deletion mutants were obtained from Dr. J. Ylanne (University of Jyvaskyla, Finland) and recloned into LZRS-IRES-zeo as described previously [92] , while Rac and RhoA mutants fused to mCherry or GFP were from Dr. J. Collard (Netherlands Cancer Institute, Amsterdam, The Netherlands). GFP-tensin-1 was from Dr. K. Yamada (National Institute of Health, Bethesda, MD), Rhotekin-RBD and PAK-CRIB peptides were home-made, human fibrinogen was from CSL Behring, collagen-coated Cytodex beads, puromycin, zeocin, human thrombin, and human FN were from Sigma-Aldrich. TRITC-, FITC-, and Cy5-conjugated secondary antibodies, phalloidins, and DAPI were from Molecular Probes, Fugene was from Promega, and HRPconjugated secondary antibodies were from Amersham.

Unless stated, all reagents were from Sigma-Aldrich (Dorset, UK). A mouse monoclonal antibody to VE-cadherin (clone 55-7H1) was from BD Biosciences (Berkshire, UK); a rabbit polyclonal antibody to ZO-1 (#61-7300) was from ThermoFisher Scientific (Loughborough, UK); a rabbit polyclonal antibody to liprin-α1 (#14175) was from Proteintech (Manchester, UK).
Alexa Fluor 488-and 594-conjugated secondary antibodies were from ThermoFisher Scientific.