For immunofluorescence analysis SH-SY5Y cells were plated in 4-well chamber slides at a density of 5 × 104 cells/well. After the differentiation and 6-OHDA treatment (protocols described in Sects. 2.4 and 2.5, respectively) cells were incubated with BDNF-PAMAM-AF488 and BDNF-PAMAM-AF488-PEG nanoparticles (0.1 μg/mL protein loading) for different time lengths (5, 10, 30 min, 1 and 24 h). At designated time points, cells were washed with PBS and fixed with 70% ethanol for 15 min. To visualize surface glycoproteins, cells were stained with wheat germ agglutinin conjugated to Texas Red-X (WGA-Texas Red-X, Thermo Fisher, Waltham, MA, USA) in HEPES buffer for 30 min. After DAPI counterstain, the slides were mounted and subjected to z-stack analysis using a LSM700 confocal system (Carl Zeiss, Jena, Germany).
Wga texas red x
Manufactured by Thermo Fisher Scientific
Sourced in United States
The WGA-Texas Red-X is a fluorescent dye that binds to glycoproteins and can be used for labeling and detection in various biological applications. It has an excitation maximum of 595 nm and an emission maximum of 615 nm, which corresponds to the Texas Red-X fluorescent label.
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Lab products found in correlation
3 protocols using wga texas red x
1
Visualizing Nanoparticle Uptake in Differentiated SH-SY5Y Cells
2
Cellular Uptake of BDNF-Loaded Nanoparticles
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For immuno uorescence analysis SH-SY5Y cells were plated in 4-well chamber slides at density 5x10 4 /well. After the differentiation and 6OHD treatment (protocols described in sections 2.4 and 2.5, respectively) cells were incubated with BDNF-PAMAM-AF488 and BDNF-PAMAM-AF488-PEG nanoparticles (0.1 μg/mL protein loading) for different time lengths (5min, 10min, 30min, 1h and 24h). At designated time points, cells were washed with PBS and xed with 70% ethanol for 15min. To visualize surface glycoproteins, cells were stained with wheat germ agglutinin conjugated to Texas Red-X (WGA-Texas Red-X, Thermo Fisher, Waltham, MA, USA) in HEPES buffer for 30min. After DAPI counterstain, the slides were mounted and subjected to z-stack analysis using a LSM700 confocal system (Carl Zeiss, Jena, Germany).
3
Cellular Uptake of BDNF-Loaded Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immuno uorescence analysis SH-SY5Y cells were plated in 4-well chamber slides at a density of 5x10 4 cells/well. After the differentiation and 6-OHDA treatment (protocols described in sections 2.4 and 2.5, respectively) cells were incubated with BDNF-PAMAM-AF488 and BDNF-PAMAM-AF488-PEG nanoparticles (0.1 μg/mL protein loading) for different time lengths (5min, 10min, 30min, 1h and 24h). At designated time points, cells were washed with PBS and xed with 70% ethanol for 15min. To visualize surface glycoproteins, cells were stained with wheat germ agglutinin conjugated to Texas Red-X (WGA-Texas Red-X, Thermo Fisher, Waltham, MA, USA) in HEPES buffer for 30min. After DAPI counterstain, the slides were mounted and subjected to z-stack analysis using a LSM700 confocal system (Carl Zeiss, Jena, Germany).
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