Recombinant proteins of human ACE2 extracellular enzymatic domain with >90% purity were purchased from R&D systems, USA (933-ZN) and Sinobiological, Inc, USA (10108-H08H). Recombinant proteins of SARS-CoV RBD (40150-V08B2) and SARS-CoV-2 RBD proteins (40592-V08H) were obtained from Sinobiological, Inc. Fluorogenic peptide substrates were obtained from R&D systems, where Mca-YVADAPK(Dnp)-OH (ES007) was used as an angiotensin II like substrate and Mca-RPPGFSAFK(Dnp)-OH(ES005) was a fluorogenic peptide derivative of Bradykinin according to manufacturer's instruction. Nonfluorogenic bradykinin(BK, RPPGFSPFR), des-Arg9-bradykinin (desBK, RPPGFSPF) and angiotensin II (Ang II, DRVYIHPF) peptides were purchased from Genscript, Inc, USA. ACE2 specific inhibitor MLN-4760 and various protease inhibitors AEBSF, pepstatin A and leupeptin were obtained from Sigma-Aldrich, Inc. Fluorogenic peptide Mca-Pro-Leu-OH (Bachem, USA, M-1975) was used as calibration standard to obtain the molarity (pmol) to relative fluorescence unit (RFU) conversion factor on Synergy H1 fluorescent plate reader.
Sars cov 2 rbd proteins 40592 v08h
Manufactured by Sino Biological
SARS-CoV-2 RBD proteins (40592-V08H) are recombinant proteins derived from the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. These proteins can be used for research purposes, such as the development of diagnostic assays and the investigation of virus-host interactions.
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Lab products found in correlation
Recombinant proteins of sars cov rbd 40150 v08b2, by Sino Biological (2 mentions)
Drvyihpf, by GenScript (2 mentions)
Mca pro leu oh, by Bachem (2 mentions)
Mca yvadapk dnp oh es007, by R&D Systems (2 mentions)
Mcarppgfsafk dnp oh es005, by R&D Systems (1 mentions)
Pepstatin a, by Merck Group (1 mentions)
Mln 4760, by Merck Group (1 mentions)
Leupeptin, by Merck Group (1 mentions)
Mca rppgfsafk dnp oh, by R&D Systems (1 mentions)
Synergy h1, by Synergy Software (1 mentions)
2 protocols using sars cov 2 rbd proteins 40592 v08h
1
ACE2-Mediated SARS-CoV-2 Inhibition Assay
2
Quantitative SARS-CoV-2 RBD Binding Assay
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Recombinant proteins of human ACE2 extracellular enzymatic domain were purchased from R&D systems(933-ZN) and Sinobiological, Inc(10108-H08H). Recombinant proteins of SARS-CoV RBD (40150-V08B2) and SARS-CoV-2 RBD proteins (40592-V08H) were obtained from Sinobiological, Inc. Fluorogenic peptide substrates were obtained from R&D systems, where Mca-YVADAPK(Dnp)-OH (ES007) was used as an angiotensin II like substrate and Mca-RPPGFSAFK(Dnp)-OH(ES005) was a fluorogenic peptide derivative of Bradykinin according to manufacturer’s instruction. Non-fluorogenic bradykinin(BK, RPPGFSPFR), des-Arg9-bradykinin (desBK, RPPGFSPF) and angiotensin II (Ang II, DRVYIHPF) peptides were purchased from Genscript, Inc. Fluorogenic peptide Mca-Pro-Leu-OH (Bachem, M-1975) was used as calibration standard to obtain the molarity (pmol) to relative fluorescence unit (RFU) conversion factor on Synergy H1 fluorescent plate reader.
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