The single cell suspension of rat tissues was prepared as previously described (He et al., 2017 (link)). After the red blood cells were lysed with ammonium-chloride-potassium buffer, the cells were stained with human or rat leukocyte-specific antibodies and analyzed with a BD LSRFortessa cytometer using FlowJo software. The antibodies used were the following: hCD45 (BD Bioscience, 561864), hCD19 (BD Bioscience, 560994), hCD3 (BD Bioscience, 563423), hCD4 (BD Bioscience, 555347), hCD8 (BD Bioscience, 555634), hCD56 (Biolegend, 318332), hCD14 (BD Bioscience, 557831), rCD45 (Biolegend, 202216), rCD45RA (Biolegend, 202318), rCD3 (Biolegend, 201403), rCD4 (Biolegend, 201516), rCD8a (Biolegend, 200610), rCD161a (BD Bioscience, 555009), hSIRPα (Biolegend, 323810), and rSIRPα (Biolegend, 204706).
Hcd14
Manufactured by BD
The HCD14 is a laboratory equipment designed to perform cell counting and analysis. It utilizes advanced optical and electronic technologies to accurately measure and enumerate various cell types within a sample. The core function of the HCD14 is to provide precise and reliable cell data to support scientific research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Lsr fortessa cytometer, by BD (1 mentions)
Hcd56, by BioLegend (1 mentions)
Hcd19, by BD (1 mentions)
Hcd45, by BD (1 mentions)
Facsaria sorp cell sorter, by BD (1 mentions)
Gentlemacs dissociator, by Miltenyi Biotec (1 mentions)
C tube, by Miltenyi Biotec (1 mentions)
Sytox green, by Thermo Fisher Scientific (1 mentions)
Dnase 1, by Roche (1 mentions)
Hla dr, by BD (1 mentions)
Collagenase d, by Roche (1 mentions)
Mcd45, by BD (1 mentions)
2 protocols using hcd14
1
Multiparametric Flow Cytometry Profiling
2
Isolation of Mouse Lung Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Single cell suspension of mouse lung harvested at p56 was prepared according to an established protocol (17 (link)). Briefly, the mouse lungs were perfused with PBS through the right ventricle. After mincing the lung tissue with scissors, the tissue was transferred to C-tubes (Miltenyi, Auburn, CA). The tissue was digested in HBSS with 1 mg/ml Collagenase D and 0.1 mg/ml DNase I (Roche, Indianapolis, IN) and dissociated with a GentleMACS dissociator (Miltenyi). The cell suspension was passed through a 40 um filter before being MACS enriched for human CD45 (Miltenyi) according to manufacturer's instructions. The cells were stained with HLA-DR, mCD45, hCD206, hCD14 (BD Biosciences, Franklin Lakes, NJ), and SYTOX Green (Thermo Fisher, Waltham, MA). FACS was performed on a BD FACSAria SORP Cell Sorter at the Northwestern Robert H. Lurie Cancer Center Flow Cytometry Core Facility. HLA-DR+, hCD206+, hCD14+, mCD45- viable cells were sorted and subsequently stained for H&E following cytospin to generate the images shown in Figure 11 .
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