Standard solutions of ZEN, α-ZOL, β-ZOL, and [13C18]-ZEN with certified concentrations of each analyte were purchased from Biopure (Tulln, Austria). ZAN, α-ZAL, and β-ZAL were purchased from Sigma (Saint Quentin Fallavier, France). Enzymes used for hydrolysis of the conjugate were purchased from Sigma and were of types H-1 β-glucuronidase (G1) from Helix pomatia (Cat# G0751), H-2 β-glucuronidase (G2) from Helix pomatia (Cat# G0875), H-1 sulfatase (S1) from Helix pomatia (Cat# S9626), H-2 sulfatase (S2) from Helix pomatia (Cat# S9751), and VI sulfatase (S3) from Aerobacter aerogenes (Cat# S1629). The immunoaffinity columns used were “easi-extract zearalenone” columns purchased from R-Biopharm Rhone LTD (Glasgow, Scotland). All other reagents were obtained from Sharlab S.L. (Sentmenat, Barcelona, Spain).
13c18 zen
Manufactured by Romer Labs
Sourced in Austria
[13C18]-ZEN is a stable isotope-labeled mycotoxin standard used for analytical purposes. It is a synthetic compound with a molecular formula of C18H22O5 and an exact mass of 334.1467 Da. This product is intended for use in research and development applications that require a reliable and well-characterized analytical standard.
Automatically generated - may contain errors
Lab products found in correlation
13c15 don, by Romer Labs (2 mentions)
β zal, by Merck Group (1 mentions)
α zol, by Romer Labs (1 mentions)
β zol, by Romer Labs (1 mentions)
α zal, by Merck Group (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
Purelab flex 2, by Elga LabWater (1 mentions)
3 acdon, by Romer Labs (1 mentions)
15 acdon, by Romer Labs (1 mentions)
Don 3g, by Romer Labs (1 mentions)
13c22 ht 2, by Romer Labs (1 mentions)
13c20 ota, by Romer Labs (1 mentions)
4 protocols using 13c18 zen
1
Zearalenone Metabolites Quantification
2
Certified Standards for Mycotoxin Analysis
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Certificated standard solutions of ZEN (100 g/mL), -ZEL (10 g/mL), -ZEL (10 g/mL), ZAN (10 g/mL), -AL (10 g/mL), -ZAL (10 g/mL) and 13 C18-ZEN (3 g/mL)
were purchased from Biopure (Tulln, Austria) and stored at -40 ºC in the dark. -glucuronidase (from E coli.) was from Sigma-Aldrich (MO, USA). Acetonitrile, methanol, ammonia acetate, formic acid and acetic acid were of LC/MS grade (Fisher Scientific, Leicestershire, United Kingdom). All other chemicals were of analytical grade or better. The deionized water (18.2 M cm) was collected from a Milli-Q system (Millipore Corp., Bedford, MA). The Oasis PRiME HLB 96-well Elution plate (3 mg of sorbent in each well) were obtained from Waters (Milford, MA, USA). A mixed standard solution containing 1 g/mL of each analyte was prepared in Acetonitrile and stored at 4 C in the dark, remaining stable for at least six months.
The working dilutions of mixed standards were prepared at each day of measurement. The enzyme solution was prepared by dissolving 14.4 mg -glucuronidase (6.9×10 5 U g solid -1 ) in 10 mL of 0.075 mol L -1 phosphate buffer (potassium phosphate dibasic + potassium phosphate monobasic, pH 6.8) freshly on the day of use.
were purchased from Biopure (Tulln, Austria) and stored at -40 ºC in the dark. -glucuronidase (from E coli.) was from Sigma-Aldrich (MO, USA). Acetonitrile, methanol, ammonia acetate, formic acid and acetic acid were of LC/MS grade (Fisher Scientific, Leicestershire, United Kingdom). All other chemicals were of analytical grade or better. The deionized water (18.2 M cm) was collected from a Milli-Q system (Millipore Corp., Bedford, MA). The Oasis PRiME HLB 96-well Elution plate (3 mg of sorbent in each well) were obtained from Waters (Milford, MA, USA). A mixed standard solution containing 1 g/mL of each analyte was prepared in Acetonitrile and stored at 4 C in the dark, remaining stable for at least six months.
The working dilutions of mixed standards were prepared at each day of measurement. The enzyme solution was prepared by dissolving 14.4 mg -glucuronidase (6.9×10 5 U g solid -1 ) in 10 mL of 0.075 mol L -1 phosphate buffer (potassium phosphate dibasic + potassium phosphate monobasic, pH 6.8) freshly on the day of use.
3
Analytical Standards Preparation for Mycotoxin Analysis
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All solvents were provided in analytical grade. Deionized water was supplied by a Purelab flex 2 (ELGA LabWater, Celle, Germany). Pure analytical standards (in acetonitrile) of DON, 15-Ac-DON, 3-Ac-DON, NIV, ZEN, TeA, AOH, AME and Tentoxin (TEN) (100 g mL ), DON-3G (50 g mL ) and -DON, -NIV, -3-Ac-DON and -ZEN (25 g mL ) were obtained from Romer Labs (Tulln, Austria). Solid standards of ALT, D3-ALT, D3-AOH, and D3-AME were purchased from HPC Standard GmbH (Cunnersdorf, Germany). From these solid standards, single analyte solutions were prepared for each native and isotopic labeled standard by dilution with methanol/water (60/40 v/v). Multicomponent solutions for constructing calibration curves of Fusarium toxins and Alternaria toxins were prepared by mixing of the single analyte standards and dilution with methanol/water (60/40 v/v); six calibration levels were prepared in the range of 10 to 1000 ng mL for each analyte. All solutions were stored at −20 C in the dark.
4
Comprehensive Mycotoxin Exposure Assessment
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This study focusses on six regulated mycotoxins deoxynivalenol (DON), ochratoxin A (OTA), zearalenone (ZEN), T-2 and HT-2 toxin (T-2, HT-2) and aflatoxin B 1 (AFB 1 ) as well as their important metabolites de-epoxy deoxynivalenol (DOM-1), nivalenol (NIV), α-zearalenol (α-ZEL), β-zearalenol (β-ZEL) and aflatoxin M 1 (AFM 1 ). Mycotoxin reference standards for all mycotoxin as well as stable isotope labelled internal standards in acetonitrile ( 13 C 15 DON, 13 C 22 HT-2, 13 C 18 ZEN, 13 C 20 OTA, ¹³C₁₇ AFB 1 ) were purchased from Romer Labs (Tulln, Austria). Urine samples from the Rowett biorepository were used in this study. Samples were collected in 2004 and stored at -20 ºC prior to analysis. Urine samples were analysed for multiple mycotoxins adapting a digestion, extraction and LC-MS/MS method published previously (29) . In brief, 4 mL of urine were spiked with a mixture of 13 C-labelled internal standards (5 ng/mL 13
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