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Genescan analysis software 3

Manufactured by Thermo Fisher Scientific

GeneScan Analysis software 3.1 is a bioinformatics software tool designed for the analysis of genetic data. It provides functionality for the processing and visualization of DNA fragment analysis data generated by genetic analysis instruments.

Automatically generated - may contain errors

2 protocols using genescan analysis software 3

1

Y-Chromosome STR Typing Using PowerPlex Ⓡ Y23 System

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PCR amplification and typing of Y-chromosome STRs targeted the following loci: DYS576, DYS389I, DYS389II, DYS448, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643, DYS393, DYS458, DYS385a, DYS385b, DYS456, and GATA-H4. Typing was performed using the Investigator PowerPlex Ⓡ Y23 System PCR Amplification kit (Promega).
Table 1 shows the positional information for each STR. The sequences of the primers used in this kit were not disclosed by the manufacturer.
Nakamura Y et al.
An ABI PRIZM 3130 Genetic Analyzer (Applied Biosystems, USA) was used to perform electrophoresis under the conditions described in the manufacturer's recommendations. Fragment sizes were determined automatically using GeneScan Analysis software 3.1 (Applied Biosystems) and the results analyzed using Genotyper version 2.5 (Applied Biosystems).
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2

Autosomal STR Profiling using Investigator Identifiler Plus

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The Investigator Identifiler Ⓡ Plus Amplification kit (Applied Biosystems) was used for PCR amplification and typing of autosomal STRs at the following loci: D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, and FGA. Table 1 shows the positional information on each STR. The primer information of this kit is not disclosed.
The ABI PRIZM 3130 Genetic Analyzer (Applied Biosystems) was used to perform electrophoresis under the conditions described in the manufacturer's recommendations. Fragment sizes were automatically determined using the GeneScan Analysis software 3.1 (Applied Biosystems) and the results analyzed using Genotyper version 2.5 (Applied Biosystems).
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