PCR amplification and typing of Y-chromosome STRs targeted the following loci: DYS576, DYS389I, DYS389II, DYS448, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643, DYS393, DYS458, DYS385a, DYS385b, DYS456, and GATA-H4. Typing was performed using the Investigator PowerPlex Ⓡ Y23 System PCR Amplification kit (Promega).
Table 1 shows the positional information for each STR. The sequences of the primers used in this kit were not disclosed by the manufacturer.
Nakamura Y et al.
An
ABI PRIZM 3130 Genetic Analyzer (Applied Biosystems, USA) was used to perform electrophoresis under the conditions described in the manufacturer's recommendations. Fragment sizes were determined automatically using
GeneScan Analysis software 3.1 (Applied Biosystems) and the results analyzed using
Genotyper version 2.5 (Applied Biosystems).
Nakamura Y., Samejima M., Minaguchi K., Nambiar P, & Hashimoto M. (2020). Population Genetics in Malaysia and Japanese Populations Using Power Plex Y23 System. The Bulletin of Tokyo Dental College, 61(2).