Donepezil hydrochloride monohydrate and scopolamine hydrobromide were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Acetylcholine ELISA kit and acetylcholinesterase ELISA kit were purchased from Elabscience Biotechnology Co. (Houston, TX, USA). Anti-β-actin antibody was purchased from Sigma Chemical Co. Anti-BDNF antibodies were purchased from Abcam (Cambridge, UK). All other materials were obtained from normal commercial sources and were of the highest grade available.
Acetylcholinesterase elisa kit
Manufactured by Elabscience
Sourced in United States
The Acetylcholinesterase ELISA Kit is a quantitative test kit designed to measure the levels of acetylcholinesterase, an enzyme that plays a crucial role in the regulation of neurotransmission. This kit provides a reliable and efficient method for the detection and quantification of acetylcholinesterase in a variety of sample types.
Automatically generated - may contain errors
2 protocols using acetylcholinesterase elisa kit
1
Acetylcholine and BDNF Assay Protocol
2
Acetylcholine Levels in Hippocampus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Briefly, mice were sacrificed by decapitation and the brain was immediately removed to isolate the cortex or hippocampus. A hippocampus tissue was added to a microfuge tube containing cold phosphate-buffered saline (PBS) and homogenized using a homogenizer. The resulting tissue was then centrifuged at 13,000 rpm and 4°C. AchE and Ach levels in the hippocampus were determined using the acetylcholine ELISA kit and acetylcholinesterase ELISA kit (Elabscience Biotechnology Co.) according to the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!