Cd38 pe cf594 clone hit2

Manufactured by BD

CD38-PE-CF594 (clone HIT2) is a fluorescently-labeled monoclonal antibody that binds to the CD38 cell surface antigen. It is designed for use in flow cytometry applications.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Cd34 pe clone 563, by BD (2 mentions) Cd45 apc clone hi30, by BD (2 mentions) Mcd45 pe cy7 clone 30 f11, by BD (2 mentions) Facsaria 2, by BD (1 mentions) Fortessa, by BD (1 mentions) Cd15 bv785 clone w6d3, by BioLegend (1 mentions) Cd19 pe clone hib19, by BD (1 mentions) Cd33 bv421 clone wm53, by BD (1 mentions) Cytofix cytoperm plus kit, by BD (1 mentions) Cd41a pe cy7 clone hip8, by BD (1 mentions) Cd33 pe cf594 clone wm53, by BD (1 mentions)

Close spelling variants of this product

CD38-PE (65 citations) CD38 (HIT2, (8 citations) CD38-PE (clone HIT2), (2 citations) CD38-PE (HIT2) (2 citations)

2 protocols using cd38 pe cf594 clone hit2

Comprehensive Immune Cell Profiling

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The following antibodies were used: CD34-PE (clone 563, BD Pharmingen), CD45-APC (clone HI30, BD PharMingen), CD33-BV421 (clone WM53, BD Horizon), mCD45-PE-Cy7 (clone 30-F11, BD Biosciences), CD19-PE (clone HIB19, BD Pharmingen), CD38-PE-CF594 (clone HIT2, BD Horizon), CD15-BV785 (clone W6D3, BioLegend), CD14-APC (clone M5E2, BD Horizon), and CD44-APC. Cell viability was assessed with DAPI (Life Technologies). Cells were assayed on a BD Fortessa and data were analyzed with FlowJo software (Tree Star). Cell sorting was performed on a BD FACSAria II.

Kotini A.G., Carcamo S., Cruz-Rodriguez N., Olszewska M., Wang T., Demircioglu D., Chang C.J., Bernard E., Chao M.P., Majeti R., Luo H., Kharas M.G., Hasson D, & Papapetrou E.P. (2023). Patient-Derived iPSCs Faithfully Represent the Genetic Diversity and Cellular Architecture of Human Acute Myeloid Leukemia. Blood Cancer Discovery, 4(4), 318-335.

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Flow Cytometry Analysis of Hematopoietic Cells

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Following hematopoietic differentiation, the cells were dissociated with accutase into single cells. The following flow cytometry antibodies were used: CD34-PE (clone 563, BD PharMingen), CD45-BV510 (clone HI30, BD Horizon), CD90-PerCP/Cy5.5 (clone 5E10, BioLegend), CD38-PE-CF594 (clone HIT2, BD Horizon), CD33-PE-CF594 (clone WM53, BD Horizon), CD49f-BV421 (clone GoH3, BD Horizon), CD45RA-APC (clone MEM56, Life Technologies), CD123-BV711 (clone 9F5, BD Horizon), CD41a- PE-Cy7 (clone HIP8, BD PharMingen), mCD45-PE-Cy7 (clone 30-F11, BD Biosciences), CD45-APC (clone HI30, BD PharMingen), CD19-PerCP-Cy5.5 (clone HIB19, BD PharMingen). Cell viability was assessed with DAPI (Life Technologies). Intracellular staining of Patient 65 cells was performed with a biotin-conjugated TSPAN18 antibody (Lifespan Bioscience) and a secondary ani-biotin APC antibody (Miltenyi Biotec) using the Cytofix/Cytoperm Plus kit (BD Biosciences). Cells were assayed on a BD Fortessa and data were analyzed with FlowJo software (Tree Star).

Wesely J., Kotini A.G., Izzo F., Luo H., Yuan H., Sun J., Georgomanoli M., Zviran A., Deslauriers A.G., Dusaj N., Nimer S.D., Leslie C., Landau D.A., Kharas M.G, & Papapetrou E.P. (2020). Acute Myeloid Leukemia iPSCs Reveal a Role for RUNX1 in the Maintenance of Human Leukemia Stem Cells. Cell reports, 31(9), 107688.

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