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Ls174t cells cl 188

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LS174T cells (CL-188) are a human colon adenocarcinoma cell line. The cells express the carcinoembryonic antigen (CEA) and can be used for research purposes.

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2 protocols using ls174t cells cl 188

1

LS174T Cell Culture Protocol

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LS174T cells (CL-188)
were obtained from American Type Culture Collection (ATCC, Manassas,
VA, USA). The cells were cultured in EMEM supplemented with 10% FBS,
100 U/mL penicillin, and 100 ng/mL streptomycin. The cells were incubated
at 37 °C in a humidified atmosphere with 5% CO2 and
95% air.
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2

Culturing LS174T and MCF7 Cells

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Human intestinal epithelial LS174T cells (CL-188) and human breast cancer MCF7 cells (HTB-22) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). The cells were cultured in EMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. The cells were then incubated at 37 °C in a humidified atmosphere with 5% CO2 and 95% air.
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