PLGA (Poly(D,L-lactide-co-glycolide), RG503H, MW ≅ 11,000) was purchased from (Evonik, Essen, Germany) and used as received. β-Glucosidase (β-Glu, MW 135 KDa), Tween® 20, 60, 80 (100%, guaranteed less than 3% water), and poly(vinyl alcohol) (PVA, MW 15,000), bovine serum albumin (BSA, 66 KDa, >98% pure), dichloromethane (DCM), 4-methylumbrelliferyl-β-D-glucopyranoside, and 4-methylumbelliferone (4-MU) were purchased from Sigma-Aldrich (Milan, Italy). MilliQ water was purified by a Millipore system (Millipore, Bedford, MA, USA). Analytical grade reagents were used for all other purposes unless otherwise noted.
Rg503h
Manufactured by Evonik
Sourced in Germany
The RG503H is a laboratory equipment product. It serves as a core function for laboratory applications. Further details about its intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Dichloromethane dcm, by Merck Group (1 mentions)
4 methylumbelliferone 4 mu, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Dimethylformamide, by Merck Group (1 mentions)
Tetrahydrofuran, by Merck Group (1 mentions)
Potassium hydroxide koh, by Merck Group (1 mentions)
Triethanolamine tea, by Merck Group (1 mentions)
2 4 dibromoacetophenone, by Merck Group (1 mentions)
Poly lactic co glycolic acid, by Evonik (1 mentions)
2 protocols using rg503h
1
PLGA Nanoparticles for β-Glucosidase Delivery
2
Quantitative PLGA Composition Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Poly(lactic-co-glycolic acid) with a GA/LA ratio of 50:50 (RG 503H) and 75:25 (RG 752H) was purchased from Evonik Nutrition & Care GmbH (Darmstadt, Germany). The reference standards for GA (99%) and LA (≥95%) were purchased from Sigma Aldrich (Darmstadt, Germany). Poly(D,L-lactic acid) (≥95%) was purchased from Polysciences, Inc. (Hirschberg an der Bergstraße, Germany). For the derivatization, 2,4′-dibromoacetophenone (≥98%) and triethanolamine (TEA) (≥98%) were purchased from Sigma Aldrich (Darmstadt, Germany). The solvents acetonitrile, acetone, tetrahydrofuran, ethyl acetate, dimethylformamide, and chloroform were all purchased from Sigma Aldrich (Darmstadt, Germany). For the hydrolysis and pH adjustment, potassium hydroxide (KOH) and aqueous hydrobromic acid (HBr) were purchased from Sigma Aldrich (Darmstadt, Germany). The pH indicator bromocresol green was purchased from Honeywell (Seelze, Germany).
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