Clinically normal appearing skin was derived from plastic surgery for breast or abdominal skin reduction after written patient consent. Ethical approval was granted by the local ethical committee (AN3694 – 279/4.3). Skin samples were trimmed off subcutaneous fat with a scalpel, and 8 mm punch biopsies (Kai Europe, Solingen, Germany) were prepared. The following mAbs were used for targeting DC in human skin: anti-DEC-205/CD205 (five different batches of clone MG38, Serotec, Kidlington, UK; and clone 523203 from R&D Systems, Minneapolis, MN, USA), anti-Langerin/CD207 (clone DCGM4/122D5.03, Dendritics, Lyon, France). Appropriately matched isotype controls (mouse IgG2b and mouse IgG1, respectively, from BioLegend, San Diego, CA, USA) were used.
Clone 523203
Manufactured by R&D Systems
Sourced in United Kingdom
Clone 523203 is a laboratory equipment product designed for specific research applications. It serves the core function of facilitating tasks essential to scientific investigation. The detailed specifications and intended use of this product are not available within the scope of this request.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using clone 523203
1
Isolation and Characterization of Human Skin Dendritic Cells
2
Isolation and Identification of Skin DCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Clinically normal appearing skin was derived from plastic surgery for breast or abdominal skin reduction after written patient consent. Ethical approval was granted by the local ethical committee (AN3694 – 279/4.3). Skin samples were trimmed off subcutaneous fat with a scalpel, and 8 mm punch biopsies (Kai Europe, Solingen, Germany) were prepared. The following mAbs were used for targeting DC in human skin: anti-DEC-205/CD205 (five different batches of clone MG38, Serotec, Kidlington, UK; and clone 523203 from R&D Systems, Minneapolis, MN, USA), anti-Langerin/CD207 (clone DCGM4/122D5.03, Dendritics, Lyon, France). Appropriately matched isotype controls (mouse IgG2b and mouse IgG1, respectively, from BioLegend, San Diego, CA, USA) were used.
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