Sc 432

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Sc-432 is a lab equipment product offered by Santa Cruz Biotechnology. It is designed for general laboratory use, but the specific core function and intended use are not available in an unbiased and factual manner without further interpretation.

Automatically generated - may contain errors

Lab products found in correlation

Sc 5279, by Santa Cruz Biotechnology (1 mentions) C6198, by Merck Group (1 mentions) Ab90967, by Abcam (1 mentions) Sc 152, by Santa Cruz Biotechnology (1 mentions) Digest all, by Thermo Fisher Scientific (1 mentions) C9080, by Merck Group (1 mentions) Sc 168, by Santa Cruz Biotechnology (1 mentions) Lsm 700, by Zeiss (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions) Ab1542, by Merck Group (1 mentions) Ab50009, by Santa Cruz Biotechnology (1 mentions) Ab9520, by Abcam (1 mentions) Ab5320, by Abcam (1 mentions) Ab123034, by Abcam (1 mentions) Ab171123, by Abcam (1 mentions) A 21245, by Thermo Fisher Scientific (1 mentions) A 21050, by Thermo Fisher Scientific (1 mentions) Lsm 700 confocal microscope, by Zeiss (1 mentions) Interferin sirna transfection reagent, by Polyplus Transfection (1 mentions)

Spelling variants of this product

Cyclin A (H-432) (sc-751; (2 citations) Anti‐PDGFRβ (sc‐432; (2 citations)

5 protocols using sc 432

Immunohistochemical Analysis of Stem Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

After routine deparaffinization and dehydration, the sections were incubated for 10 min. at 37°C with 1% trypsin (Digest All, cat# 003008; Invitrogen, Darmstadt, Germany) for antigen unmasking. After washing with PBS, the tissues were blocked with 5% BSA for 1 hr at RT and incubated with the appropriate primary antibodies diluted in 3% BSA with 0.2% TritonX-100 in PBS overnight at 4°C. Mouse monoclonal Oct-3/4 (C-10), dilution 1:50 (sc5279; Santa Cruz), mouse monoclonal anti-vimentin, 1:500 (C 9080; Sigma-Aldrich), monoclonal anti-α-actin smooth muscle conjugated with Cy3 (α-SMA), 1:500 (C6198; Sigma-Aldrich), anti-PDGFR-α, 1:100 (ab90967; Abcam, Cambridge, UK), rabbit polyclonal PDGFR-β, 1: 100 (sc-432; Santa Cruz), rat antimouse Ly-6A/E/Sca-1, 1:100 (cat # 553333; BD Biosciences, Heidelberg, Germany), rabbit polyclonal C-kit, 1:100 (sc-168; Santa Cruz), rabbit polyclonal VEGF, 1:100 (sc-152; Santa Cruz) were used for the staining. The sections where then incubated with the appropriate secondary antibodies (1:500–1000) for 1 hr at RT, washed with PBS and counterstained with DAPI (1:1000) for 10 min. The slices were mounted with Mowiol and investigated with a Zeiss LSM 710 laser scanning confocal microscope.

Galiger C., Kostin S., Golec A., Ahlbrecht K., Becker S., Gherghiceanu M., Popescu L.M., Morty R.E., Seeger W, & Voswinckel R. (2014). Phenotypical and ultrastructural features of Oct4-positive cells in the adult mouse lung. Journal of Cellular and Molecular Medicine, 18(7), 1321-1333.

+ Open protocol

+ Expand

Pericyte Labeling and Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pericytes were labelled by expression of DsRed under control of the NG2 promoter (in mice), or with anti-NG2 (Merck Millipore AB5320 1:200 or AbCam ab50009 1:200) or anti-PDGFRβ (Santa Cruz Biotechnology sc-432 1:200) antibodies (in rats), and the capillary basement membrane was labelled with isolectin B₄ congugated to Alexa Fluor 647 (Molecular Probes, I32450) or FITC (Sigma-Aldrich, UK, L2895) as described (Mishra et al., 2014 (link)). Pericyte association with sympathetic terminals was imaged using antibody to tyrosine hydroxylase (Merck Millipore AB1542 1:500). Z-stacks for cell counting were acquired using laser-scanning confocal microscopy (Zeiss LSM 700). Pericyte intersoma distance was calculated between pairs of pericytes on capillaries within the same imaging plane. Antibodies to α-SMA, β-actin and γ₁ actin were AbCam ab5694 (1:100), Abbiotec 251815 (1:100) and AbCam ab123034 (1:100). Red blood cells were labelled with antibody to glycophorin A (AbCam ab9520, 1:2000). Neutrophils were labelled with antibodies to neutrophil elastase (Santa Cruz, sc9521 (M-18), 1:50) or ICAM1 (Abcam, Ab171123, 1:100).

O'Farrell F.M., Mastitskaya S., Hammond-Haley M., Freitas F., Wah W.R, & Attwell D. (2017). Capillary pericytes mediate coronary no-reflow after myocardial ischaemia. eLife, 6, e29280.

+ Open protocol

+ Expand

Multimodal Immunostaining of Brain Slices

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human and rat brain slices were fixed in 4% paraformaldehyde (PFA) for 1 hour, washed 3 times in phosphate-buffered saline (PBS), then blocked in 10% goat serum/0.5% Triton X100 in PBS. Primary antibodies for PDGFRβ (Santa Cruz, sc432, 1:200) or α-SMA (Santa Cruz, CGA7, 1:200) or Aβ (IBL, 82E1, 1:500) were applied overnight, followed (after washing in PBS) by application overnight of Alexa Fluor 647 or 633 conjugated secondary antibodies (ThermoFisher, A-21245, A-21070, A-21050, 2 μg/ml). Slices were then washed once in PBS containing DAPI nuclear stain (1:50,000) for 10 mins and then washed again in PBS. After mounting, slices were imaged on a Zeiss LSM700 confocal microscope.

Nortley R., Korte N., Izquierdo P., Hirunpattarasilp C., Mishra A., Jaunmuktane Z., Kyrargyri V., Pfeiffer T., Khennouf L., Madry C., Gong H., Richard-Loendt A., Huang W., Saito T., Saido T.C., Brandner S., Sethi H, & Attwell D. (2019). Amyloid β oligomers constrict human capillaries in Alzheimer’s disease via signaling to pericytes. Science (New York, N.Y.), 365(6450), eaav9518.

+ Open protocol

+ Expand

Silencing PDGFRβ in Mesenchymal Stem Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

cMSCs were transfected with 30 nM mouse PDGFRβ (or control) small interfering RNA (siRNA) (Santa Cruz Biotechnology) using INTERFERin siRNA transfection reagent (Polyplus Transfection) according to the manufacturer’s instructions. After 96 hours of transfection, cMSCs were evaluated for PDGFRβ expression by immunoblotting using standard protocols as previously described.²² (link)^,²³ (link) In other experiments, siRNA–transfected cMSCs were trypsinized, washed, and counted 24 hours’ post-transfection followed by the collagen gel contraction assay as described earlier. Primary antibodies used for immunoblotting were purchased from Santa Cruz Biotechnology, including against PDGFRα (sc-338), PDGFRβ (sc-432), alpha smooth muscle actin (α-SMA) (sc-58669), α-tubulin (sc-8035), vinculin (sc-736), and glyceraldehyde-3-phosphate dehydrogenase (sc-32249).

Hamid T., Xu Y., Ismahil M.A., Rokosh G., Jinno M., Zhou G., Wang Q, & Prabhu S.D. (2022). Cardiac Mesenchymal Stem Cells Promote Fibrosis and Remodeling in Heart Failure: Role of PDGF Signaling. JACC: Basic to Translational Science, 7(5), 465-483.

+ Open protocol

+ Expand

Immunohistochemical Analysis of TLS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen ST biopsy samples were cut in 5-mm sections and mounted on Star Frost adhesive glass slides (Knittelgläser, Braunschweig, Germany). Sections were fixed with acetone. Endogenous peroxidase activity was blocked with 0.3% H 2 O 2 in 0.1% sodium azide in phosphate-buffered saline for 20 minutes. Sections were stained with mouse monoclonal primary antibodies against NIK (sc-8417, Santa Cruz Biotechnology, Santa Cruz, CA), FDC-M1 (14-9968-80, eBioscience, San Diego, CA), rabbit polyclonal primary antibodies against PDGFRb (sc-432; Santa Cruz Biotechnology), and the secondary antibodies goateanti-mouse (p0447; Dako, Glostrup, Denmark) or swineeanti-rabbit (p0399; Dako) and streptavidin labeled with horseradish peroxidase. Biotinylated tyramide was used for amplification, as previously described. 30 As a negative control, isotype-matched immunoglobulins were applied to the sections instead of the primary antibody. Expression was scored semiquantitatively on a scale of 0 to 4 by two blinded researchers independently (A.R.N. and K.P.M.v.Z.). Semiquantitative scoring was used because digital image analysis is less suitable for comparing tissue with TLS versus tissue without TLS because information on the location of the cells in the tissue is lost.

Noort A.R., van Zoest K.P., van Baarsen L.G., Maracle C.X., Helder B., Papazian N., Romera-Hernandez M., Tak P.P., Cupedo T, & Tas S.W. (2015). Tertiary Lymphoid Structures in Rheumatoid Arthritis: NF-κB-Inducing Kinase-Positive Endothelial Cells as Central Players. The American journal of pathology, 185(7).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!