Tetraethylthiuram disulfide

Molybdenum hexacarbonyl Mo(CO)₆ (98.0%), tetraethylthiuram disulfide (≥97%), ammonium tetrathiotungstate (≥99.99%), sodium diethyldithiocarbamate trihydrate (≥98%), sulfur (≥99.98%), ammonium sulfide (20 wt% in water), tetraethylammonium bromide (≥99%), ammonium perrhenate (≥99.0%), bis(diethylthiocarbamoyl)disulfide (≥97%), manganese(II) acetate tetrahydrate (≥99%), chromium(III) chloride hexahydrate (≥96%), and silica gel (40–63 µm particle size) were purchased from Sigma–Aldrich and used without further purification. Acetone (≥99.0%) dichloromethane (≥99.0%), methanol (≥99.5%), diethyl ether (≥99.0%), acetonitrile (≥99.9%), hexane (≥97.0%), dry acetonitrile (≥99.80%), pentane (≥99.0%), isopropanol (≥99.5%), and hydrochloric acid (37%) were purchased from Sigma–Aldrich and used as received.

BIX-01294 (2-(hexahydro-4-methyl-1H-1,4-diazepin-1-yl)-6,7-dimethoxy-N-[1-(phenylmethyl)-4-piperidinyl]-4-quinazolinamine trihydrochloride hydrate), SAHA (suberoylanilide hydroxamic acid), HMBA (N,N′-hexamethylene bis(acetamide)), disulfiram (tetraethylthiuram disulfide), bryostatin-1, and JQ1 were all purchased from Sigma-Aldrich. The positive controls Pam3Csk4 and poly(I:C) were purchased from InvivoGen.

Sauchinone (over 98% purity) was provided by Professor Chin Y.-W. (Pharmacognosy Laboratory of Dongguk University, Seoul, Korea) according to the modification of previously reported method [48 (link)]. Hydroxybupropion, 4-hydroxymephenytoin, 6-hydroxychlorzoxazone, S-mephenytoin, and pooled human liver microsomes from a mixed pool of 50 donors (HLMs; BD Ultra Pool HLM 50, cat. 452156) were purchased from BD Gentest Co. (Woburn, MA, USA). Acetaminophen, chlorzoxazone, clopidogrel, coumarin, furafylline, 6α-hydroxytestosterone, 7-hydroxycoumarin, ketoconazole, 4-methylpyrazole, omeprazole, paroxetine, phenacetin, quinidine, sibutramine, sulfaphenazole, testosterone, tetraethylthiuram disulfide, ticlopidine, tienilic acid, tolbutamide, tranylcypromine, verapamil, and reduced form of NADPH (as a tetrasodium salt) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dextrorphan and 4-hydroxytolbutamide were purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). Bupropion, carbamazepine (internal standard, IS), dextromethorphan, fluvoxamine, fluoxetine, and 8-methoxypsoralen were purchased Wako Co. (Tokyo, Japan). All other chemicals and reagents used were of analytical grade.