[11C]harmine (7-[11C]methoxy-1-methyl-9H-[3,4-b]indole) synthesis and quality control was performed according to procedures described by Philippe et al.23 (link) All PET data was acquired using a GE Advance full-ring scanner at the PET Center at the Medical University of Vienna and in accordance with the center’s standard protocols. For tissue attenuation correction, a transmission scan of 5 min was carried out with 68GE rod sources. An intravenous bolus of [11C]harmine (4.6 MBq/kg body weight) was administered simultaneously with the start of PET. Dynamic PET was performed in 3D mode and reconstructed in 35 transaxial section volumes (128 × 128 matrix) with an iterative filtered backprojection algorithm (FORE-ITER) with a spatial resolution of 4.36 mm full-width at half maximum next to the center of the field of view. Fifty one successive time frames (12 frames of 5 s, 6 frames of 10 s, 3 frames of 20 s, 6 frames of 30 s, 9 frames of 1 min and 15 frames of 5 min) were collected, resulting in a total acquisition time of 90 min, optimized for modeling of [11C]harmine. Arterial blood samples for [11C]harmine quantification were drawn automatically for the first 10 min and manually at standardized intervals after [11C]harmine injection15 (link).
Advance full ring scanner
Manufactured by GE Healthcare
The Advance full-ring scanner is a state-of-the-art medical imaging device designed for healthcare applications. It features a complete circular ring of detectors that provide high-quality imaging capabilities. The core function of this equipment is to capture precise diagnostic images for healthcare professionals.
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Lab products found in correlation
2 protocols using advance full ring scanner
1
Synthesis and Imaging of [11C]Harmine
2
Synthesis and PET Imaging of [11C]Harmine
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Synthesis and quality control of [ 11 C]harmine, (7-[ 11 C]methoxy-1-methyl-9H-pyrido [3,4-b] indole) was performed as published by our group [51] . All PET scans were carried out using a GE Advance full-ring scanner situated at the PET Centre, Division of Nuclear Medicine, at the Medical University of Vienna. For attenuation correction a transmission scan of 5 min was performed with 68 Ge rod sources. An intravenous bolus of [ 11 C]harmine (4.6 MBq/kg body weight) was administered simultaneously with the start of the dynamic PET scan. PET scans were acquired in 3D mode with a total scanning time of 90 min, separated into 51 optimized time frames, a spatial resolution of 4.36 mm full-width at half-maximum one cm next to the centre of the field of view and reconstructed in 35 transaxial section volumes with an iterative filtered backprojection algorithm (128 Â 128 matrix). Automatic arterial blood sampling was carried out continuously for the first 10 min at a rate of 4 ml/min (ALLOGG, Mariefred, Sweden) and manually at 5, 10, 20, 30, 45, 60, and 80 min after [ 11 C]harmine injection [38] . A crosscalibrated gamma counter was used to calculate radioactivity concentrations in plasma. Radioactive metabolites of the tracer were determined using high-performance liquid chromatography (HPLC) [52] .
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