All chemicals used were of analytical grade. Ethanol, Methanol, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), Acetone, Aluminium Chloride, Ammonium Acetate, Catechin, Copper (II) Chloride Dehydrate, Ferric Chloride, Folin-Ciocalteur Reagent, Gallic Acid, Glacial Acetic Acid, Hexane, Hydrochloric Acid, Neocuprine, Potassium Hexacyanoferrate (II), Sodium Acetate Trihydrate, Sodium Carbonate, Sodium Hydrogen Sulphite, Sodium Hydroxide, Sodium Nitrate, and Zinc Acetate were purchased from Sigma-Aldrich (Castle Hill, Sydney, Australia). Nutrient Agar Plates and Nutrient Broth were purchased from Thermo Fisher Scientific (Thebarton, South Australia, Australia). Deionised (DI) water was collected following purification (Millipore Australia, North Ryde, NSW), with a resistivity higher than 18 MΩcm−1.
Neocuprine
Manufactured by Merck Group
Sourced in Australia
Neocuprine is a laboratory equipment product manufactured by Merck Group. It is designed for use in various scientific and research applications. The core function of Neocuprine is to facilitate precise and accurate measurements in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium acetate, by Merck Group (3 mentions)
Sodium carbonate, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Copper 2 chloride dehydrate, by Merck Group (2 mentions)
Folin ciocalteu reagent, by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Ferric chloride, by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
Nutrient agar plate, by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Nutrient broth, by Thermo Fisher Scientific (1 mentions)
Sodium nitrate, by Merck Group (1 mentions)
Zinc acetate, by Merck Group (1 mentions)
Sodium acetate trihydrate, by Merck Group (1 mentions)
Aluminium chloride, by Merck Group (1 mentions)
Potassium hexacyanoferrate 2, by Merck Group (1 mentions)
5 protocols using neocuprine
1
Comprehensive Phytochemical Analysis
2
Phytochemical Profiling and Antioxidant Activity
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Acetic acid, ethanol 96%, methanol, and sucrose were from Chempur (Piekary Śląskie, Poland). HPLC-grade methanol and acetonitrile were purchased in Merck (Darmstadt, Germany).
Plant culture media components, plant growth regulators BA (6-benzyladenine) and NAA (1-naphthaleneAcetic acid) and agar were purchased in Duchefa Biochemie (Haarlem, Netherlands). Cultures were grown in the plant tissue-dedicated glass containers (V8630, Sigma-Aldrich, Saint Louis, MI, USA).
Commercially available standards: chlorogenic acid, cryptochlorogenic acid, and neochlorogenic acid, hyperoside (quercetin 3-galactoside), isoquercitrin (quercetin 3-glucoside), isorhamnetin, kaempferol, guaijaverin (quercetin 3-arabinoside), quercetin, rutoside (quercetin 3-rutinoside), and trifolin (kaempferol-3-galactoside) of HPLC grade (≥95.0%) purity were acquired in Sigma-Aldrich Saint Louis, MI, USA. Ammonium acetate, CuCl2⋅2H2O, DPPH, FeCl3⋅6H2O, Folin-Ciocalteu reagent, hydrochloric acid (HCl), Na2CO3, 2,9-dimethyl-1,10-phenanthroline (neocuprine), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (trolox) were also provided by Sigma-Aldrich. Deionised water (>15 MΩ) was produced in house (PureLab OptionR, Elga, High Wycombe, UK).
Plant culture media components, plant growth regulators BA (6-benzyladenine) and NAA (1-naphthaleneAcetic acid) and agar were purchased in Duchefa Biochemie (Haarlem, Netherlands). Cultures were grown in the plant tissue-dedicated glass containers (V8630, Sigma-Aldrich, Saint Louis, MI, USA).
Commercially available standards: chlorogenic acid, cryptochlorogenic acid, and neochlorogenic acid, hyperoside (quercetin 3-galactoside), isoquercitrin (quercetin 3-glucoside), isorhamnetin, kaempferol, guaijaverin (quercetin 3-arabinoside), quercetin, rutoside (quercetin 3-rutinoside), and trifolin (kaempferol-3-galactoside) of HPLC grade (≥95.0%) purity were acquired in Sigma-Aldrich Saint Louis, MI, USA. Ammonium acetate, CuCl2⋅2H2O, DPPH, FeCl3⋅6H2O, Folin-Ciocalteu reagent, hydrochloric acid (HCl), Na2CO3, 2,9-dimethyl-1,10-phenanthroline (neocuprine), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (trolox) were also provided by Sigma-Aldrich. Deionised water (>15 MΩ) was produced in house (PureLab OptionR, Elga, High Wycombe, UK).
3
Antioxidant Activity Assay Protocol
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Chemicals used in this study included Ethanol, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-n-propylthiouracil (PROP), Ammonium Acetate, Copper (II) Chloride Dehydrate, Folin-Ciocalteu Reagent, Gallic Acid, Neocuprine, and Sodium Carbonate and were purchased from Sigma-Aldrich (Castle Hill, Sydney, Australia). The DI water was prepared using the Millipore water purification system (Millipore Australia, North Ryde, NSW, Australia).
4
Antioxidant Capacity Determination by CUPRAC
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The CUPRAC method [32 (link)] was slightly modified by Biesaga-Kościelniak et al. [33 ]. Lyophilized materials were ground to a uniform powder. Antioxidants were extracted with 1 mL of methanol (#32213-M, Sigma-Aldrich, Poznań, Poland) from 5 mg samples (15 min, 30 Hz; MM400, Retch, Haan, Germany). Samples were centrifuged (5 min. at 22,000 × g, UniversalR32, Hettich, Tuttlingen, Germany), 50 µL of the supernatant was pipetted to wells filled 50 µL of 10 mmol L−1 Cu2+ (#307483, Sigma-Aldrich, Poznań, Poland), 7.5 mM neocuprine (#N1501, Sigma-Aldrich, Poznań, Poland), and 1 mol L−1 (pH 7.0) ammonia-acetate (#238074, Sigma-Aldrich, Poznań, Poland) buffer. After 15 min incubation at 25 °C absorbance at 425 nm was recorded (Synergy 2, Winooski, VT, USA). The content of antioxidants was calculated as Trolox equivalent antioxidant capacity (TEAC) in mmol (100 g)−1 of dry weight (DW).
5
Polyphenol Quantification Protocol
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Monohydrate citric acid, ethanol (96% and absolute), acetone, chlorohydric acid (37%), sodium hydroxide (pellets), methanol (analytic grade), ammoniac (analytic grade), Folin–Ciocâlteu reagent, and monohydrate gallic acid were purchased from Scharlau (Barcelona, Spain). The 1-methylimidazole was obtained from Merck (Barcelona, Spain). Glacial acetic acid and sorbitan monolaurate (Tween® 20) were obtained from Panreac AppliChem (Barcelona, Spain). Neocuprine, 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulphonic acid) and potassium bromide were purchased from Sigma-Aldrich (Madrid, Spain).
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