Hairpin it mirna qpcr kit
The Hairpin-it™ miRNA qPCR kit is a laboratory equipment product designed for the quantitative detection of microRNA (miRNA) expression using real-time PCR (qPCR) technology. The kit provides a standardized and efficient method for miRNA quantification.
2 protocols using hairpin it mirna qpcr kit
Gene Expression Analysis by qRT-PCR
Quantitative Analysis of lncRNA and miRNA Expression
Primer sequences used for qRT-PCR
Gene | Primer sequences |
---|---|
SNHG16 | |
F | 5ʹ-CAGAATGCCATGGTTTCCCC-3ʹ′ |
R | 5ʹ-TGGCAAGAGACTTCCTGAGG-3ʹ |
miR-302b-3p | |
Loop | 5′-GTCGTATCCAGTCCAGGGACCGAGGACTGGATACGACCTACTA-3′ |
F | 5′-GCGTAAGTGCTTCCATGTT-3′ |
R | 5′-TCCAGGGACCGAGGA-3′ |
SLC2A4 | |
F | 5′-TGGCTGGGTTCTCCAACTG-3′ |
R | 5′-CTGGAAACCCATGCCAATG-3′ |
β-actin | |
F | 5′-ACCGAGCGCGGCTACAG-3′ |
R | 5′-CTTAATGTCACGCACGATTTCC-3′ |
U6 snRNA | |
F | 5′ -CTCGCTTCGGCAGCACA-3′ |
R | 5′-AACGCTTCACGAATTTGCGT-3′ |
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