Reagents and materials were from Fisher Scientific (Pittsburgh, PA) or Sigma-Aldrich (St. Louis, MO) unless stated otherwise. Antibody to CP was mouse mAb clone 2A3 for immunoblots4 (link). Other antibodies and sources were as follows: Fam21C (rabbit pAb) from Millipore (Billerica, MA), anti-CD2AP (rabbit pAb) was a kind gift from Dr. Andrey Shaw of Washington University, Goat anti-Dap12 from Santa Cruz (Dallas, TX), anti-GFP (rabbit pAb) and Rabbit anti-GST antibody from Abcam (Cambridge, MA), and anti-FLAG (mouse mAb M2) from Sigma Aldrich. Dynabeads, Protein G, HRP- and Alexa-conjugated secondary antibodies and Alexa 488- and Alexa 568-conjugated phalloidin were from Life Technologies (Carlsbad, CA). Arp2/3 complex inhibitors CK-666 and CK-86939 (link), along with the control compound CK-689, were purchased from Sigma-Aldrich. pERFPC-1 and pEGFPC-1 were obtained from Clontech (Mountain View, CA). PIP2 was obtained from Avanti Polar Lipids (Alabaster, AL).
Alexa 488 and alexa 568 conjugated phalloidin
Manufactured by Thermo Fisher Scientific
Alexa 488- and Alexa 568-conjugated phalloidin are fluorescent stains used to label and visualize actin filaments in cells. Phalloidin is a toxin that binds specifically to F-actin, and the Alexa Fluor dyes provide bright fluorescent labeling of the actin cytoskeleton. These products are commonly used in fluorescence microscopy applications to study cell structure and dynamics.
Automatically generated - may contain errors
Lab products found in correlation
Dynabead, by Thermo Fisher Scientific (2 mentions)
Pegfp c1, by Takara Bio (2 mentions)
Ck 689, by Merck Group (2 mentions)
Anti gfp, by Abcam (2 mentions)
Fam21c, by Merck Group (2 mentions)
Anti cd2ap, by Santa Cruz Biotechnology (2 mentions)
Goat anti dap12, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti gst antibody, by Abcam (2 mentions)
Hrp and alexa conjugated secondary antibodies, by Thermo Fisher Scientific (2 mentions)
Perfpc 1, by Takara Bio (2 mentions)
Ck 666, by Merck Group (1 mentions)
Anti flag m2 mouse mab, by Merck Group (1 mentions)
Anti flag, by Merck Group (1 mentions)
2 protocols using alexa 488 and alexa 568 conjugated phalloidin
1
Immunoblot Analysis of CP-Associated Proteins
2
Antibody-based Immunoprecipitation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents and materials were from Fisher Scientific (Pittsburgh, PA) or Sigma-Aldrich (St Louis, MO), unless stated otherwise. Antibody to CP was mouse monoclonal antibody clone 2A3 for immunoblots4 (link). Other antibodies and sources were as follows: Fam21C (rabbit pAb) from Millipore (Billerica, MA), anti-CD2AP (rabbit pAb) was a kind gift from Dr Andrey Shaw of Washington University, goat anti-Dap12 from Santa Cruz (Dallas, TX), anti-GFP (rabbit pAb) and rabbit anti-GST antibody from Abcam (Cambridge, MA) and anti-FLAG (mouse monoclonal antibody M2) from Sigma-Aldrich. Dynabeads, Protein G, HRP- and Alexa-conjugated secondary antibodies and Alexa 488- and Alexa 568-conjugated phalloidin were from Life Technologies (Carlsbad, CA). Arp2/3 complex inhibitors CK-666 and CK-869 (ref. 39 (link)), along with the control compound CK-689, were purchased from Sigma-Aldrich. pERFPC-1 and pEGFPC-1 were obtained from Clontech (Mountain View, CA). PIP2 was obtained from Avanti Polar Lipids (Alabaster, AL).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!