Nk 92mi cells

NK-92MI cells were obtained from Procell Life Science&Technology Co, Ltd. They were cultures in αMEM(Gibco, Cat#41061029) medium supported with 12.5% heat-inactivated FBS(Hyclone, Cat#SH30088.03HI), 12.5% heat-inactivated horse serum (Gibco, Cat#26050088), 0.2 mM inositol (Solarbio, Cat#I8050), 0.1 mM 2-mercaptoethanol (Solarbio, Cat#M8210), and 0.02 mM folic acids (Solarbio, Cat#P1400). All media were supplemented with 100 U/mL penicillin and 100 mg/mL streptomycin (Solarbio, Cat#P1400). Cells were cultivated at 37 °C in a humidified atmosphere of 5% CO₂ in the air.

Orah mandarins were bought from Guangxi Hyperion International Agricultural Logistics Co., Ltd. (Nanning, China). HPLC-grade mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, fucose, N-acetyl glucosamine and N-acetyl galactosamine were provided by Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). NK-92MI cells (natural killer cells in patients with human malignant non-Hodgkin’s lymphoma), Calu-1 cells (human lung cancer cells), MEMα medium, and McCoy’s 5A medium were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). TRIzol Reagent was obtained from Thermo Fisher Scientific (Waltham, MA, USA). HiScript II QRT SuperMix for qPCR (+gDNA wiper) and ChamQ Universal SYBR qPCR Master Mix were acquired from Vazyme Biotech Co., Ltd. (Nanjing, China). All compounds were analytical grade unless otherwise specified.

The human OC cell line SKOV3, OV-90 and COC1/DDP (cisplatin resistant) were obtained from the China Center for Type Culture Collection. NK92/MI cells and human ovarian epithelial cells IOSE80 were obtained from Procell Life Science&Technology Co., Ltd. SKOV3, OV-90, COC1/DDP and IOSE80 were cultured in Mc-Coy’s 5a medium, DMEM/F12 medium (Gibco, Gaithersburg, MD, USA), RPMI 1640 medium (Gibco), and DMEM/High Glucose medium (HyClone, USA), respectively. All cultural media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Gibco). NK92/MI was cultured in MEM-Alpha medium (Gibco) supplemented with 12.5% FBS, 12.5% horse serum, 0.2mM Inositol, 2-Mercaptoethanol and 0.02 mM folic acid (Sigma-Aldrich, St. Louis, MO, USA). All cells were cultured at 37°C in a humidified atmosphere of 5% CO₂-95% air.
To obtain NK cells treated with OC ascites (AS-t-NK), the ascites collected from 7 patients (Supplementary Table S1) with malignant OC were aliquoted into 50-mL conical tubes and centrifuged at 300 g for 10 min to separate cell pellets from supernatant (24 (link)). eNK cells were then cultured in RPMI 1640 medium containing 10% OC ascites for 24 h.