Mosquito midguts were stored in 180 µL DNA Tissue Lysis buffer (Roche Diagnostics, Australia) spiked with a known amount of EHV. 20 µL of Proteinase K was added to the lysis mixture, followed by incubation overnight at 56 °C. Total nucleic acid was extracted from the midgut lysates using the DNA and Viral NA Small Volume Kit on the MagNA Pure 96 instrument (Roche Diagnostics, Australia) following the manufacturer’s protocol (DNATissue SV2.0). Total nucleic acid was eluted into 100 µL and stored at − 80 °C until PCR was performed.
Dna tissue lysis buffer
Manufactured by Roche
Sourced in Australia
The DNA Tissue Lysis buffer is a laboratory reagent designed to extract and prepare DNA from tissue samples. It is used to lyse, or break down, the cell membranes and release the genetic material within. The buffer contains a combination of chemicals that facilitate this process, enabling the downstream isolation and purification of DNA for further analysis or experimentation.
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Lab products found in correlation
2 protocols using dna tissue lysis buffer
1
Mosquito Midgut DNA Extraction
2
Mosquito Feeding Assay for Malaria Transmission
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Mosquitoes were fed on gametocytaemic blood from volunteers enrolled in a CHMI transmission study via either direct skin feeding or membrane feeding assays [18 (link)]. Mosquitoes were dissected for evaluation of oocysts on day 8 or 9 after feeding assay. Midguts were collected in 180 µL of DNA Tissue Lysis buffer (Roche Diagnostics, Australia) for PCR analysis, or were stained with 0.5% mercurochrome for visualization of oocysts by microscopy prior to collection in lysis buffer and PCR analysis.
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