3 4 5 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt neutral red solution

Dulbecco′s Modified Eagle Medium (DMEM) high glucose, trypsin-ethylenediaminetetraacetic acid, trypan blue solution 0.4% (w/v), sodium dodecyl sulphate, hydrochloric acid (HCl), sodium bicarbonate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), neutral red (NR) solution, Hoescht 33258 solution, dimethyl sulfoxide, retinoic acid, paraformaldehyde, carbonyl cyanide 3-chlorophenylhydrazone (CCCP), ACRO, and FBAL were obtained from Sigma-Aldrich (Taufkirchen, Germany). 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbo-cyanine iodide (JC-1) was obtained from Thermo Fisher Scientific (Waltham MA, USA). Phosphate-buffered saline (PBS) without calcium and magnesium and penicillin/streptomycin were obtained from Biochrom (Berlin, Germany). Foetal bovine serum (FBS), Hanks’ balanced salt solution (HBSS) were acquired to Gibco (Paisley, UK). Doxorubicinol hydrochloride was obtained from Tebu-bio (Lisbon, Portugal). All plastic sterile material used in cell culture was obtained from Corning-Costar (Corning, NY, USA).

MTX, DOX, trypsin-EDTA solution, trypan blue solution 0.4% (w/v) and Dulbecco’s modified Eagle medium (DMEM) high glucose, sodium bicarbonate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), neutral red (NR) solution, Hoechst 33258 solution, 3,3′-dihexyloxacarbocyanine iodide (DiO6), dimethyl sulfoxide (DMSO), RA, TPA, NAC, tiron, CHX, Ac-DEVD-CHO, an inhibitor of caspase-3/7, and paraformaldehyde were obtained from Sigma-Aldrich (Taufkirchen, Germany). Human neuroblastoma SH-SY5Y cells were obtained from the European Collection of Cell Cultures (Sigma-Aldrich, Taufkirchen, Germany). All sterile plastic material was obtained from Corning Costar (Corning, NY, USA). Penicillin/streptomycin (10.000 units/mL/10.000 μg/mL) and the phosphate buffer solution (PBS) without calcium and magnesium were obtained from Biochrom (Berlin, Germany). Fetal bovine serum (FBS), PBS with calcium and magnesium and Hank’s balanced salt solution (HBSS) were obtained from Gibco (Paisley, UK).