Mhc 2

Manufactured by Cytek Biosciences

Sourced in United States

MHC II is a laboratory instrument used for the detection and analysis of major histocompatibility complex (MHC) class II proteins. It provides researchers with the ability to measure and characterize these important cell surface molecules, which play a crucial role in the adaptive immune response.

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Lab products found in correlation

Pd l1, by BioLegend (1 mentions) Mhc 1, by BioLegend (1 mentions) Anti cd25 biotin, by Thermo Fisher Scientific (1 mentions) Cd11b, by Cytek Biosciences (1 mentions)

3 protocols using mhc 2

Comprehensive Immunophenotyping Protocol

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The following monoclonal antibodies (mAb) were obtained from BioLegend (San Diego, CA, USA): MHC-I (Alexa Fluor 647, clone AF6-88.5), CD40 (PE/Cy5, clone 3/2.3), PD-L1 (PE/Cy7, clone MIH5), IDO (clone mIDO-48) and an ELISA MAX standard set for mouse IFN-γ, IL-12 (p70), IL-10, and TNF-α. From Tonbo Biosciences (San Diego, CA. USA) mAb to CD11c (PE, clone N418); MHC-II (APC, clone APC 114.15.2); CD80 (FITC, clone 16-10A1); CD86 (APC, clone GL-1); CD25 (PE/Cy7, clone PC61.5); CD4 (PE, clone GK1.5). From eBioscience (San Diego, CA, USA): anti-CD25 (Clone 61.5) and anti-CD25 biotin (clone eBio7D4).

Magallanes-Puebla A., Espinosa-Cueto P., López-Marín L.M, & Mancilla R. (2018). Mycobacterial glycolipid Di-O-acyl trehalose promotes a tolerogenic profile in dendritic cells. PLoS ONE, 13(12), e0207202.

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Multiparameter Flow Cytometry Panel

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The following fluorochrome-conjugated mAb were purchased from Biolegend: Ly6C (HK1.4), Nkp46 (29A1.4), MHC II (I:A/I:E) (M5/114.15.2), CD62L (MEL-14), CD45.2 (104), CD44 (IM7), Ly6G (1A8), TLR2 (QA16A01), TLR4-MD2 (MTS510), CD64 (X54–5/7.1, CD14 (Sa14–2) and Tonbo Biosciences: CD8a (53–6.7), CD4 (RM4–5), IFNγ (XMG1.2), Ghost 780 (live/dead).

Huggins M.A., Sjaastad F.V., Pierson M., Kucaba T.A., Swanson W., Staley C., Weingarden A.R., Jensen I.J., Danahy D.B., Badovinac V.P., Jameson S.C., Vezys V., Masopust D., Khoruts A., Griffith T.S, & Hamilton S.E. (2019). Microbial Exposure Enhances Immunity to Pathogens Recognized by TLR2 but Increases Susceptibility to Cytokine Storm through TLR4 Sensitization. Cell reports, 28(7), 1729-1743.e5.

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Intravital Imaging of GR-ZIF Accumulation

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For GR-ZIF accumulation studies, C57BL/6 female mice were intradermally injected with XenoLight DiR (excitation/emission, 710/760 nm) or D-ZIF-8 in the right forelimb of hairless mice. For flow cytometry analysis, the brachial dLN was harvested and dissociated using a spleen dissociation medium according to the manufacturer’s instructions. Cells were centrifuged and resuspended in a buffer containing zombie violet, CD45, CD11c (Tonbo Biosciences, 910004), CD11b (Tonbo Biosciences, 910004), CD8 (Tonbo Biosciences, 910004), and MHC II (Tonbo Biosciences, 910004). Cells were then washed and resuspended in PBS + 2% BSA.

Alsaiari S.K., Nadeef S., Daristotle J.L., Rothwell W., Du B., Garcia J., Zhang L., Sarmadi M., Forster T.A., Menon N., Lin S.Q., Tostanoski L.H., Hachmann N., Wang E.Y., Ventura J.D., Barouch D.H., Langer R, & Jaklenec A. (2024). Zeolitic imidazolate frameworks activate endosomal Toll-like receptors and potentiate immunogenicity of SARS-CoV-2 spike protein trimer. Science Advances, 10(10), eadj6380.

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