Rabbit PGAM5 antibody was purchased from Novus (NBP1, 92,257); Mouse PGAM5 (Santa, sc515880); Rabbit PINK1 (Proteintech, 23,274-1-AP) and translocase of the outer mitochondrial membrane 20 (TOMM20, Proteintech, 11,802-AP), translocase of the inner mitochondrial membrane 23 (TIMM23, Proteintech, 11,123-1-AP), light chain 3 (LC3, Proteintech, 14,600-1-AP); β-Actin (Proteintech, 20,536-1-AP); Mouse microtubule-associated protein 2 (MAP2, SAB, 38022) and glial fibrillary acidic protein (GFAP, SAB, 38014); Mouse ionized calcium-binding adapter molecule 1 (IBA1, Servicebio, O70200). The antibodies used in this study were used according to the instructions, and the molecular weights observed in WB were consistent with the literature (Wu et al., 2018 (link); Wang et al., 2020 (link); Gao et al., 2021 (link); Brunelli et al., 2022 (link)).
Timm23
Manufactured by Proteintech
TIMM23 is a protein that functions as a component of the translocase of the inner mitochondrial membrane. It plays a role in the import and sorting of nuclear-encoded proteins into the mitochondria.
Automatically generated - may contain errors
Lab products found in correlation
Tomm20, by Proteintech (1 mentions)
β actin, by Proteintech (1 mentions)
Total oxphos cocktail, by Abcam (1 mentions)
Cytochrome c, by BD (1 mentions)
Bnip3, by Abcam (1 mentions)
Mtch2, by Proteintech (1 mentions)
Mitofilin, by Proteintech (1 mentions)
Hsp60, by Proteintech (1 mentions)
Lonp1, by Proteintech (1 mentions)
Tom70, by Proteintech (1 mentions)
Tom20, by ABclonal (1 mentions)
Flag tag (flag), by Merck Group (1 mentions)
Actin, by Merck Group (1 mentions)
2 protocols using timm23
1
Mitochondrial Dynamics Analyzed by Western Blotting
2
Multiparametric Mitochondrial Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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BNIP3 (Abcam, ab109362; Abcam, ab10433; Cell signaling, 3769S), NIX (Cell signaling, 12396S), FLAG (Sigma-Aldrich, F1804), HA (Sigma-Aldrich, H6533), ACTIN (Sigma-Aldrich, A2066), TOM70 (Proteintech, 14528-1-AP), TOM20 (ABclonal, A19403), SMAC (Cell signaling, 2954S), MITOFILIN (Proteintech, 10179-1-AP), HSP60 (Cell signaling, 4870S), TIMM23 (Proteintech, 11123-1-AP), SKP1 (Cell signaling, 2156S), CUL1 (Proteintech, 12895-1-AP), Total OXPHOS Cocktail (Abcam, ab110411), Cytochrome C (BD Biosciences, 556433), mCherry (Easybio, BE2026), MTCH2 (Proteintech, 16888-1-AP), BECLIN1 (Cell signaling, 3495T), FIP200 (ABclonal, A14685), TFAM (Abcam, ab131607), LONP1 (Proteintech, 15440-1-AP), VDAC (Cell signaling, 4866S), PARP (Cell signaling, 9542S), Donkey polyclonal anti-Rabbit IgG (H+L), , Donkey polyclonal anti-Mouse IgG (H+L), HRP-conjugated (Jackson ImmunoResearch, 711-035-151), Goat polyclonal anti-Mouse IgG, Fcγ fragment specific, HRP-conjugated (Jackson ImmunoResearch, 115-035-008), Goat polyclonal anti-Mouse IgG, light chain specific, HRPconjugated (Jackson ImmunoResearch, .
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