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Anti pig3

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Anti-PIG3 is a primary antibody that specifically binds to the PIG3 protein. PIG3 is a p53-inducible protein involved in the regulation of apoptosis. The antibody can be used for the detection of PIG3 expression in various applications, such as Western blotting and immunohistochemistry.

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Lab products found in correlation

Anti rbbp5, by Fortis Life Sciences (2 mentions) Anti ash2l, by Fortis Life Sciences (2 mentions) Anti apaf1, by R&D Systems (2 mentions) Alexa fluor goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Alexa fluor 680 goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) Anti tubulin, by Merck Group (2 mentions) Anti h3k4me3, by Abcam (2 mentions) Cycletest plus dna reagent kit, by BD (2 mentions) Anti p21, by BD (2 mentions)

2 protocols using anti pig3

1

Comprehensive Western Blotting and Cell Cycle Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was performed as described previously 8 (link), 61 (link), with the exception that Alexa Fluor secondary antibodies were used. All blots were developed using the Odyssey fluorescence image scanner and the band intensities were quantified using LI–COR software. Cell cycle analysis was performed using PI (propidium iodide) staining as described previously 8 (link), using the CycleTEST PLUS DNA reagent kit (BD Biosciences, San Jose, CA, USA; # 340242). The following antibodies were used in the study: anti–P53 (SCBT; sc–6243), anti–P21 (BD; #556431), anti–APAF1 (R & D; #MAB828), anti–PIG3 (abcam; #ab64798), anti–PUMA (CST; #4976); anti–Ash2L (Bethyl Labs; #A300-108A), anti–H3K4me3 (abcam; #ab1012), anti–Wdr5 (abcam; #ab56919); anti–RbBP5 (Bethyl Labs; #A300-109A), anti–RNAP II (SCBT; sc–899); anti–Serine5–CTD (abcam; #ab5131–50); anti–TAFII (SCBT; sc–735); anti–TFIIB (SCBT; sc–225); anti–TFIIF (SCBT; sc–235); anti–Tubulin (Sigma; #T5168), Alexa Fluor 680 goat anti–mouse IgG (Molecular Probes; #A21057) and Alexa Fluor goat anti–rabbit IgG (Molecular Probes; #A21076).
Mungamuri S.K., Wang S., Manfredi J.J., Gu W, & Aaronson S.A. (2014). Ash2L enables P53–dependent apoptosis by favoring stable transcription pre–initiation complex formation on its pro-apoptotic target promoters. Oncogene, 34(19), 2461-2470.
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2

Comprehensive Western Blotting and Cell Cycle Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was performed as described previously 8 (link), 61 (link), with the exception that Alexa Fluor secondary antibodies were used. All blots were developed using the Odyssey fluorescence image scanner and the band intensities were quantified using LI–COR software. Cell cycle analysis was performed using PI (propidium iodide) staining as described previously 8 (link), using the CycleTEST PLUS DNA reagent kit (BD Biosciences, San Jose, CA, USA; # 340242). The following antibodies were used in the study: anti–P53 (SCBT; sc–6243), anti–P21 (BD; #556431), anti–APAF1 (R & D; #MAB828), anti–PIG3 (abcam; #ab64798), anti–PUMA (CST; #4976); anti–Ash2L (Bethyl Labs; #A300-108A), anti–H3K4me3 (abcam; #ab1012), anti–Wdr5 (abcam; #ab56919); anti–RbBP5 (Bethyl Labs; #A300-109A), anti–RNAP II (SCBT; sc–899); anti–Serine5–CTD (abcam; #ab5131–50); anti–TAFII (SCBT; sc–735); anti–TFIIB (SCBT; sc–225); anti–TFIIF (SCBT; sc–235); anti–Tubulin (Sigma; #T5168), Alexa Fluor 680 goat anti–mouse IgG (Molecular Probes; #A21057) and Alexa Fluor goat anti–rabbit IgG (Molecular Probes; #A21076).
Mungamuri S.K., Wang S., Manfredi J.J., Gu W, & Aaronson S.A. (2014). Ash2L enables P53–dependent apoptosis by favoring stable transcription pre–initiation complex formation on its pro-apoptotic target promoters. Oncogene, 34(19), 2461-2470.
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