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V plex assay plates

Manufactured by Mesoscale
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The V-Plex Assay Plates are a multi-well plate system designed for multiplex immunoassays. The plates feature pre-coated capture antibodies for the simultaneous measurement of multiple analytes in a single sample. The plates are compatible with various detection methods and can be used in a wide range of research applications.

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Mpo standard curve, by Merck Group (2 mentions)

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V-plex assays (13 citations)

3 protocols using v plex assay plates

1

Colon Tissue Cytokine Quantification

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Post-mortem colon tissue was homogenised using a method adapted from processing lung tissue (Mangan et al. 2006 (link)). Levels of pro-inflammatory cytokines, such as INF-γ, IL-6, IL1-β, TNF-α, and IL-10, were detected using V-Plex Assay Plates (Meso Scale Diagnostics; Rockville, MD, USA) and assayed as per the manufacturer’s protocol. MPO activity was detected using o-phenylenediamine dihydrochloride as substrate and the data were interpolated from an MPO standard curve (Sigma). Levels of cytokines and MPO were expressed as pg per mg or U per mg, respectively, relative to colon protein (Cummins et al. 2008 (link)).
Khan M.N., Lane M.E., McCarron P.A, & Tambuwala M.M. (2017). Caffeic acid phenethyl ester is protective in experimental ulcerative colitis via reduction in levels of pro-inflammatory mediators and enhancement of epithelial barrier function. Inflammopharmacology, 26(2), 561-569.
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2

Plasma Biomarker Analysis Protocol

Cited 1 time
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Blood samples at baseline and on cycle 2 day 1 (+/− 2 days, approximately 4 weeks on treatment) were collected into EDTA tubes, separated within 2 hours, and stored in aliquots at −80°C until use. Quantitative analysis of plasma VEGF, IL-6, IL-8 and soluble VEGFR2 concentrations were performed using clinically validated custom V-PLEX assay plates on an electrochemiluminescence (ECL) platform, according to manufacturer’s instructions (Meso Scale Discovery, Gaithersburg, MD) [20 (link)]. Purified recombinant proteins and reference samples were included in all assays for standard curves, performance validation, and for determining analyte concentrations in the plasma samples.
Lee J.M., Annunziata C.M., Hays J.L., Cao L., Choyke P., Yu M., An D., Turkbey I.B., Minasian L.M., Steinberg S.M., Chen H., Wright J, & Kohn E.C. (2020). Phase II trial of bevacizumab and sorafenib in recurrent ovarian cancer patients with or without prior-bevacizumab treatment. Gynecologic oncology, 159(1), 88-94.
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3

Colonic Cytokine and MPO Quantification

Cited 1 time
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The colonic tissue lysate was blended and homogenised as described by [23 (link)]. The expression of pro-inflammatory cytokines INF-γ, IL-6 and TNF-α, were quantified using V-Plex Assay Plates (Meso Scale Diagnostics; Rockville, MD, USA) and analyse as per the manufacturer’s. MPO activity was detected using o-phenylenediamine dihydrochloride as substrate and the data were interpolated from an MPO standard curve (Sigma). Expression of cytokines and MPO was represented as per milligram or U per milligram, respectively, relative to colonic protein.
Tambuwala M.M., Khan M.N., Thompson P, & McCarron P.A. (2018). Albumin nano-encapsulation of caffeic acid phenethyl ester and piceatannol potentiated its ability to modulate HIF and NF-kB pathways and improves therapeutic outcome in experimental colitis. Drug Delivery and Translational Research, 9(1), 14-24.
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