3h nα methylhistamine

UCL-2190^{41 (link)}, Ciproxifan⁴² and Pitolisant^{27 (link)} were from own laboratory stocks of which synthesis and analytics have been described previously. G9a-inhibitors A-366 and UNC-0642 as well as G9a enzyme, S-adenosylmethionine (SAM, (2S)-2-amino-4-[[(2S,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl-methylsulfonio]butanoate), biotinylated histone H3 (1–21) fragment and Dulbecco´s modified eagle medium (DMEM, article no. D5671) were purchased from Sigma-Aldrich, Taufkirchen, Germany. Fetal bovine serum albumin (FBS Good-Forte) and Dulbecco´s Phosphate Buffered Saline (DPBS) were provided by PAN biotech (Aidenbach, Germany). The radioligands [³H]N^α-methylhistamine, [³H]histamine, [³H]spiperone and [³H]SCH23390 were purchased from PerkinElmer (Rodgau, Germany) as well as AlphaLISA materials such as AlphaLISA H3K9me2 acceptor beads, streptavidin-coated donor beads, detection buffer (5x) and white 384-well microplates (OptiPlate). Human or animal blood/tissue/cell samples have not been used in this study.

Dubelcco’s Modified Eagles Medium (DMEM), Phosphate Buffered Saline (PBS), Trizma Base, polyethyleneimine solution (50%, PEI) were purchased from Sigma Aldrich (Saint Louis, MO, USA). Fetal Bovine Serum (FBS, Bodinco BV, Alkmaar, The Netherlands), penicillin/Streptomycin (streptomycin 10,000 IU·mL⁻¹; penicillin 10,000 μg·mL⁻¹, Thermo Fischer Scientific, (p/a Perbio Science BVBA, Etten-Leur, The Netherlands) , linear 25 kDa polyethyleneimine (PEI, Polysciences, Warrington, PA, USA), [³H]-N-α-methylhistamine (specific activity 79.7 Ci/mmol, Perkin Elmer, (Waltham, MA, USA), thioperamide (Abcam, Cambridge, UK) histamine (TCI, Nihonbashi-honcho, Chuo-ku, Tokyo, Japan).