Total protein was extracted from the pancreatic and intestinal tissues of mice, and the protein concentration was determined using a bicinchoninic acid assay. The protein samples were separated through sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Bio-Rad, Hercules, CA). The membranes were then blocked with 10% skim milk–Tris-buffered saline with Tween 20 (TBST) solution at room temperature for 1.5 h, followed by an overnight incubation at 4 °C with the corresponding primary antibodies (IL-6 antibody diluted at 1:1000, IL-1β antibody diluted at 1:1000, TNF- antibody diluted at 1:2000, β-actin antibody diluted at 1:5000, and GAPDH antibody diluted at 1:5000, all purchased from Proteintech). The membranes were rinsed with TBST solution in triplicate and then incubated with the corresponding secondary antibodies conjugated with horseradish peroxidase at room temperature for 1 h. Chemiluminescent signals were detected using the Tanon-5200 chemiluminescence imaging system. The signal from each protein band was quantified using the ImageJ software.
Il 1β antibody
Manufactured by Proteintech
Sourced in China
The IL-1β antibody is a laboratory tool used to detect and measure the presence of the interleukin-1 beta (IL-1β) protein. IL-1β is a pro-inflammatory cytokine involved in various cellular processes. This antibody can be used in techniques such as ELISA, Western blotting, and immunohistochemistry to quantify and analyze IL-1β expression in samples.
Automatically generated - may contain errors
Lab products found in correlation
Il 6 antibody, by Proteintech (2 mentions)
β actin antibody, by Proteintech (2 mentions)
Il 18 antibody, by Proteintech (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Tnf antibody, by Proteintech (1 mentions)
Gapdh antibody, by Proteintech (1 mentions)
Polyvinylidene difluoride membrane, by Bio-Rad (1 mentions)
Nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
Cleaved caspase 1 antibody, by Santa Cruz Biotechnology (1 mentions)
Tnf α antibody, by Proteintech (1 mentions)
Asc antibody, by Cell Signaling Technology (1 mentions)
Cse antibody, by Proteintech (1 mentions)
Nlrp3 antibody, by Cell Signaling Technology (1 mentions)
P nf κb p65 antibody, by Cell Signaling Technology (1 mentions)
Inos antibody, by BD (1 mentions)
Total nf κb p65, by Cell Signaling Technology (1 mentions)
Rosiglitazone, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
4 protocols using il 1β antibody
1
Protein Analysis in Mouse Tissues
2
Evaluating Inflammatory Pathways in Cellular Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following compounds were used in this study: LPS (cat. L2880, Sigma-Aldrich, USA), GO (cat. W250309, Sigma-Aldrich), IAM (cat. A3221, Sigma-Aldrich), AOAA (cat. C13408, Sigma-Aldrich), and PAG (cat. P7888, Sigma-Aldrich). Antibodies used were IL-6 antibody (cat. 21865-1-AP, Proteintech, China), TNF-α antibody (cat. 60291-1-Ig, Proteintech), IL-1β antibody (cat. 16806-1-AP, Proteintech), IL-18 antibody (cat. 10663-1-AP, Proteintech), p-NFκB p65 antibody (cat. 3033, Cell Signaling Technology, USA), NF-κB p65 antibody (cat. 8242T, Cell Signaling Technology), NLRP3 antibody (cat. 15101S, cat. 8242T, Cell Signaling), ASC antibody (cat. sc-514414, Santa Cruz Biotechnology, USA), cleaved caspase-1 antibody (cat. sc56036, Santa Cruz Biotechnology), GSDMD antibody (cat. A18281, Abclonal, USA), cleaved N-terminal GSDME antibody (cat. ab222407, Abcam, UK), CBS antibody (cat. GTX628777, GeneTex, USA), CSE antibody (cat. 12217-1-AP, Proteintech), 3MST antibody (cat. HPA001240, Sigma-Aldrich), and β-actin antibody (60004-1-Ig, Proteintech).
3
Adrenomedullin Protocol for Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ADM (molecular formula: C₂₄₂H₃₈₁N₇₇O₇₅S₅; sequence: H‐Tyr‐Arg‐Gln‐Ser‐Met‐Asn‐Gln‐Gly‐Ser‐Arg‐Ser‐Thr‐Gly‐Cys‐Arg‐Phe‐Gly‐Thr‐Cys‐Thr‐Met‐Gln‐Lys‐Leu‐Ala‐His‐Gln‐Ile‐Tyr‐Gln‐Phe‐Thr‐Asp‐Lys‐Asp‐Lys‐Asp‐Gly‐Met‐Ala‐Pro‐Arg‐Asn‐Lys‐Ile‐Ser‐Pro‐Gln‐Gly‐Tyr‐NH2) was purchased from Bachem (Bubendorff, Switzerland). IBMX, dexamethasone, rosiglitazone, and insulin were from Sigma‐Aldrich. LPS, PKA inhibitor P9115, and ADM receptor antagonist ADM22‐52 were from Anaspec (Fremont, California). DMEM, FBS, 0.25% trypsin‐EDTA, streptomycin/penicillin, and trypsin were obtained from Thermo Fisher Scientific (Pudong New District, Shanghai, China). The ADM, CRLR, RAMP2/3, and TNFα antibodies were from Abcam (Cambridge, UK). The IL‐1β antibody was obtained from Proteintech (SANYING, Wuhan, China). The COX‐2 antibody was from Cayman Chemical (AmyJet Scientific Inc., Hongshan, Wuhan, China). The iNOS antibody was purchased from BD Biosciences (Pudong New District, Shanghai, China). The phosphor‐IκBα, total‐IκBα, phosphor‐NF‐κB p65, total‐NF‐κB p65, phospho‐p38, total‐p38 MAPK, phospho‐ERK1/2, total‐ERK1/2, phospho‐JNK, and total‐JNK antibodies were obtained from Cell Signaling Technology (Shanghai) Biological Reagents, Inc. (Pudong New District, Shanghai, China).
4
Comprehensive Histological Assessment of Atherosclerosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse aortas were opened longitudinally from the ascending aorta to the abdominal aorta, and fixed in 4% paraformaldehyde for 36 h. Hematoxylin and eosin (HE) staining assay was used to detect atherosclerotic lesions, and the Oil Red O (Solarbio, China) staining kit was used to evaluate lipid accumulation. Masson staining was used to evaluate the content of collagen fibers in atherosclerotic plaques. Immunofluorescence staining was performed to stain the location of endothelial cells in atherosclerotic lesions using CD31 antibody (1:100, Abcam, USA), and to detect the expression of GSDMD (1:50, Santa Cruz, USA) and the co-localization of endothelial NLRP3 (1:100, AdipoGen, USA) and ASC (1:100, AdipoGen, USA) by visualizing using a fluorescence microscope (Olympus, Japan). Immunohistochemistry staining was performed to stain the level of IL-1β and IL-18 in atherosclerotic lesions using IL-1β antibody (1:100, Proteintech, China) and IL-18 antibody (1:100, Proteintech, China) by visualizing using a Nano Zoomer (Hamamatsu, Japan). Quantitative analysis of lesions was performed using Fiji software (NIH, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!