Whole blood samples were withdrawn from mice and stained with Alexa Fluor 633–coupled anti-Gr1 antibody (clone RB6-8C5, purified from hybridoma supernatant), PE-coupled anti-CD41 antibody (clone MWReg30; BD Biosciences), FITC-coupled anti-Ly6B2 antibody (clone 7/4; AbD Serotec), and PerCP-coupled anti-CD45 antibody (clone 30-F11; BD Biosciences). Platelet–neutrophil aggregates were quantified by measuring the percentage of CD41+ neutrophils (CD45+Gr1+7/4+) using a flow cytometer (BD FACSCantoII; BD Biosciences). To analyze aggregates of platelets and T reg cells, whole blood samples were stained with Alexa Fluor 633–coupled anti-CD4 antibody (clone GK1.5; BioLegend), PE-coupled anti-CD41 antibody (clone MWReg30; BD Biosciences), FITC-coupled anti-CD25 antibody (clone PC61; BioLegend), and PerCP-coupled anti-CD45 antibody (clone 30-F11; BD Biosciences). Platelet–T reg cell aggregates were quantified using a flow cytometer (BD FACSCantoII; BD Biosciences).
Pe coupled anti cd41 antibody clone mwreg30
Manufactured by BD
The PE-coupled anti-CD41 antibody (clone MWReg30) is a laboratory reagent used for the detection and analysis of CD41-expressing cells. It is a fluorescently-labeled monoclonal antibody that binds specifically to the CD41 antigen, also known as the platelet glycoprotein IIb. This antibody can be used in flow cytometry and other immunoassay applications to identify and quantify CD41-positive cells.
Automatically generated - may contain errors
2 protocols using pe coupled anti cd41 antibody clone mwreg30
1
Quantifying Platelet-Immune Cell Aggregates
2
Quantification of Platelet-Neutrophil Aggregates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole blood samples were withdrawn from mice and stained with Alexa633-coupled anti-Gr1 antibody (clone RB6-8C5, dilution 1:100), PE-coupled anti-CD41 antibody (clone MWReg30, dilution 1:100, BD Biosciences), FITC-coupled anti-Ly6B2 antibody (clone 7/4, dilution 1:100, AbD Serotec, Düsseldorf, Germany), and PerCP-coupled anti-CD45 antibody (clone 30-F11, dilution 1:100, BD Biosciences). Platelet-neutrophil aggregates were quantified by measuring the percentage of CD41+ neutrophils (CD45+Gr1+7/4+) using a flow cytometer (BD FACSCanto II, BD Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!