Anti stx17 hpa001204

Manufactured by Merck Group

Sourced in United States

Anti-STX17 (HPA001204) is a laboratory reagent produced by Merck Group. It is an antibody that specifically binds to the STX17 protein, which is involved in autophagosome formation. This product can be used for research purposes to detect and study the STX17 protein.

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STX17 (3 citations) STX17 (HPA001204 (2 citations) Anti-Stx17 (2 citations)

3 protocols using anti stx17 hpa001204

Comprehensive Antibody Collection for Cell Signaling

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Antibodies used in this study included anti-STING (13647; Cell Signaling Technology), anti-Flag M2 (F3165; Sigma-Aldrich), anti-HA (H3663; Sigma-Aldrich), anti-EGFP (GL-8; Clontech), anti-LC3 (7543; Sigma-Aldrich), anti-p62 (PM045; MBL), anti-STX17 (HPA001204; Sigma-Aldrich), anti-SNAP29 antibody (111303, SYSY, or sc-135564; Santa Cruz Biotechnology), anti-LAMP1(L1418; Sigma-Aldrich), anti-LAMP2 (sc-18822; Santa Cruz or L0668; Sigma-Aldrich), anti-Myc (9E10, DSHB), anti-WIPI2 (ab105459; Abcam), anti-FIP200 (17250; ProteinTech), anti-ATG16L (PM040; MBL), anti-α-Tubulin (E7; DSHB), anti-VAMP8 (ab76021; Abcam), anti-GABARAP (ab109364; Abcam), anti-AMPK (2532; Cell Signaling Technology), anti-p-AMPK T172 (2535; Cell Signaling Technology), anti-TBC1D1(66433; Cell Signaling Technology), anti-p-TBC1D1 Ser237(07-2268; Sigma-Aldrich), anti-Raptor (2280; Cell Signaling Technology), anti-p-Raptor Ser792 (2083; Cell Signaling Technology), anti-TSC2 (4308; Cell Signaling Technology), anti-p-TSC2 Ser1387 (5584; Cell Signaling Technology), anti-ACC (3662; Cell Signaling Technology), anti-p-ACC Ser79 (3661; Cell Signaling Technology), anti-Glut4 (GT-41-A; Alpha diagnostic international), anti-Laminin2 (L0663; Sigma-Aldrich), anti-CD36 (80080; Abcam), anti-Dystrophin (15277; Abcam), and LysoTracker (ENZ-51005; Enzo).

Rong Y., Zhang S., Nandi N., Wu Z., Li L., Liu Y., Wei Y., Zhao Y., Yuan W., Zhou C., Xiao G., Levine B., Yan N., Mou S., Deng L., Tang Z., Liu X., Kramer H, & Zhong Q. (2022). STING controls energy stress-induced autophagy and energy metabolism via STX17. The Journal of Cell Biology, 221(7), e202202060.

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Immunoblotting and Immunofluorescence Techniques

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Mouse monoclonal anti-LC3 (clone 1703; Cosmo Bio Co., Tokyo, Japan), anti–β-actin (clone AC74; Sigma-Aldrich, Tokyo, Japan), anti-FLAG (clone M2; Sigma-Aldrich), anti-Myc (9E10; Covance Research Products, Denver, PA), anti-EGF receptor (610017; BD Biosciences, Franklin Lakes, NJ), anti-EEA1 (M176-3; MBL, Tokyo, Japan), rat monoclonal anti-GFP antibody (clone GF090R; Nacalai Tesque, Kyoto, Japan), rabbit polyclonal anti-GFP (598; MBL), anti-p62 (PM045; MBL), anti-STX17 (HPA001204; Sigma-Aldrich), anti-VPS33A (C1C3; GeneTex, Irvine, CA), anti-VPS39 (ab107570; Abcam, Cambridge, MA), and anti-UVRAG (A301-996A; Bethyl Laboratories, Montgomery, TX) antibodies were used. The rabbit polyclonal antibodies against LC3 (LC3 #1) for immunoblotting (Hosokawa et al., 2006 (link)) and Atg16L1 (Mizushima et al., 2003 (link)) have been described previously. Rabbit polyclonal anti-LAMP1 antibody was provided by Yoshitaka Tanaka (Kyushu University, Fukuoka, Japan). Rabbit polyclonal anti-VPS16 antibody was provided by Chihiro Akazawa (Tokyo Medical and Dental University; Kim et al., 2001 (link)). Alexa 488–conjugated anti-mouse, rabbit, and rat immunoglobulin G (IgG), Alexa 568–conjugated anti-mouse and rabbit IgG, and Alexa 660–conjugated anti-mouse and rabbit IgG secondary antibodies were purchased from Molecular Probes (Eugene, OR).

Jiang P., Nishimura T., Sakamaki Y., Itakura E., Hatta T., Natsume T, & Mizushima N. (2014). The HOPS complex mediates autophagosome–lysosome fusion through interaction with syntaxin 17. Molecular Biology of the Cell, 25(8), 1327-1337.

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Autophagy Pathway Protein Analysis

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Anti-Atg14 (5504S, Cell Signaling, Danvers, MA, USA), Anti-LAMP2 (PRS3627, Sigma-Aldrich, St. Louis, MO, USA), Anti-LAMP2 (ab13524, Abcam, Cambridge, UK), Anti-LC3 (SAB1305552, Sigma-Aldrich), Anti-LC3 (66139-1-lg, Proteintech, Rosemont, IL, USA), Anti-mTOR (#2972, Cell Signaling), Anti-Phospho-mTOR (Ser2481) (#2974S, Cell Signaling), Anti-Phospho-mTOR (Ser2448) (#5536, Cell Signaling), Anti-p62 (ab155686, Abcam), Anti-STX17 (HPA001204, Sigma-Aldrich).

Hua R., Han S., Zhang N., Dai Q., Liu T, & Li J. (2018). cPKCγ-Modulated Sequential Reactivation of mTOR Inhibited Autophagic Flux in Neurons Exposed to Oxygen Glucose Deprivation/Reperfusion. International Journal of Molecular Sciences, 19(5), 1380.

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