Analytical standards of amlodipine (AML) were purchased from Sigma-Aldrich (Poznań, Poland). LC-MS grade methanol (MeOH) was obtained from Sigma-Aldrich (Poznań, Poland). Formic acid was from Merck (Warsaw, Poland). Ultrapure water was obtained from a HLP5 system (Hydrolab, Poland). MeOH used for extraction were LC grade and were purchased from Merck (Warsaw, Poland). Individual stock solution of AML at concentration level of 1 g L−1 was prepared in methanol and was stored at −80°C.
Lc ms grade methanol meoh
Manufactured by Merck Group
Sourced in Germany, Poland, United Kingdom
LC-MS grade methanol (MeOH) is a high-purity solvent commonly used in liquid chromatography-mass spectrometry (LC-MS) applications. It serves as a mobile phase component to facilitate the separation and ionization of analytes during mass spectrometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Merck Group (6 mentions)
Ammonium acetate, by Merck Group (3 mentions)
Hlp5 system, by Hydrolab (2 mentions)
Lc ms water, by Merck Group (2 mentions)
4 hydroxybenzoic acid, by Merck Group (2 mentions)
Quercetin, by Merck Group (2 mentions)
P coumaric acid, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
Ferulic acid, by Merck Group (2 mentions)
Vanillic acid, by Merck Group (2 mentions)
Syringic acid, by Merck Group (2 mentions)
Amlodipine, by Merck Group (1 mentions)
Lysophosphatidylcholine lpc 19 0, by Avanti Polar Lipids (1 mentions)
Milli q ultrapure system, by Merck Group (1 mentions)
Acetonitrile acn, by Merck Group (1 mentions)
Myricetin, by Extrasynthese (1 mentions)
Kaempferol, by Extrasynthese (1 mentions)
Epicatechin, by Extrasynthese (1 mentions)
Catechin, by Extrasynthese (1 mentions)
Epigallocatechin, by Extrasynthese (1 mentions)
16 protocols using lc ms grade methanol meoh
1
Amlodipine Quantification Protocol
2
Skeletal Muscle Fiber Type Analysis
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LC-MS grade methanol (MeOH) and LC-MS water were purchased from Sigma-Aldrich (St Louis, MO). Lipid standards lyso-phosphatidylcholine (LPC) 19:0, lyso-phosphatidylethalonamine (LPE) 17:1, lyso-phosphatidylglycerol (LPG) 17:1, lyso-phosphatidylserine (LPS) 17:1 and lyso-phosphatidylinositol (LPI) 17:1 were purchased from Avanti Polar Lipids (Alabaster, AL). Arachidonic acid (AA)-d8 standard was purchased from Cayman (Ann Arbor, MI).
Primary antibodies used for fiber types staining were purchased from Developmental Studies Hybridoma Bank (DSHB) (Iowa City, IA): Myh7 MyHC-1 (BA-F8) for type I fiber, Myh2 MyHC-2A (2F7) for type IIA fiber, and Myh4 MyHC-2B (10F5) for type IIB fiber. Secondary antibodies used in the staining were purchased from Thermo Fisher Scientific (Waltham, MA): goat anti-mouse IgG1, Alexa Fluor 568 (A-21124); goat anti-mouse IgG2b, Alexa Fluor 647 (A-21242); goat anti-mouse IgM, Alexa Fluor 488 (A-210420).
Primary antibodies used for fiber types staining were purchased from Developmental Studies Hybridoma Bank (DSHB) (Iowa City, IA): Myh7 MyHC-1 (BA-F8) for type I fiber, Myh2 MyHC-2A (2F7) for type IIA fiber, and Myh4 MyHC-2B (10F5) for type IIB fiber. Secondary antibodies used in the staining were purchased from Thermo Fisher Scientific (Waltham, MA): goat anti-mouse IgG1, Alexa Fluor 568 (A-21124); goat anti-mouse IgG2b, Alexa Fluor 647 (A-21242); goat anti-mouse IgM, Alexa Fluor 488 (A-210420).
3
Bisphenol Compound Preparation and Quantification
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BPA (CAS 80-05-7, purity 98.5%), BPA-d4 (purity > 97.8%) BPB (CAS 77-40-7, purity > 98.5%), BPF (CAS 620-92-8, purity > 99.0%), BPAF (CAS 1478-61-1, purity 98.0%), and BPS (CAS 80-09-1, purity > 98%) were supplied by Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). BPF-d10 (purity > 99%) and BPS-13C12 (purity > 99%) were purchased from Toronto Research Chemical Inc. (Ontario, Canada), and BPAF-d4 (purity > 99%) was available from CDN Isotopes Inc. (Quebec, Canada). BPA-d4 (purity > 99%) and BPB-13C12 (purity > 99%) were obtained from Cambridge Isotope Laboratories Inc. (Andover, MA). LC-MS grade methanol (MeOH) and acetonitrile (ACN) were offered by Sigma−Aldrich (St. Louis, MO). Ultrapure water was obtained from a Milli-Q ultrapure system (Millipore, Bedford, MA, USA). Phosphate-buffered saline (PBS, 0.2 M) was purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). The stock standard solutions (10 μg/mL) were individually prepared by dissolving in MeOH and were stored at −20°C. Working solutions were prepared by serial dilution of stock solutions with MeOH/water (50 : 50, v/v).
4
Analytical Standards Preparation for LC-MS
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Analytical standards of IBP, KET, and FUR were purchased from Sigma-Aldrich (Poznań, Poland). LC-MS grade methanol (MeOH) was obtained from Sigma-Aldrich (Poznań, Poland). Formic acid was from Merck (Warsaw, Poland). Ultrapure water was obtained from an HLP5 system (Hydrolab, Poland). MeOH used for extraction were LC grade and were purchased from Merck (Warsaw, Poland). Individual stock solutions of each compound at concentration level of 1 g/L were prepared in methanol and were stored at −80 °C.
5
Quantification of Polyphenolic Compounds
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Standards of cyanidin, malvidin-3-glucoside, cyanidin 3-O glucoside, epigallocatechin, epicatechin, petunidin 3-O glucoside, pelargonidin 3-0 glucoside, peonidin 3-O glucoside, delphinidin 3-O glucoside, catechin, resveratrol, rutin, kaempferol, and kaempferol 3-O glucoside were purchased from Extrasynthese (Geney, France); myricetin, quercetin, quercetin 3-O glucoside, gallic acid, p-coumaric acid, ferulic acid, caffeic acid, trans-cinnamic acid, vanillic acid, 4-hydroxybenzoic acid, 3,5-dihydroxybenzoic acid, chlorogenic acid, and syringic acid were purchased from Sigma-Aldrich (St Louis, MO, USA). LC-MS grade methanol (MeOH) 99.99%, ethanol (EtOH) 99.7% and formic acid (FA) 98% were all obtained from Sigma-Aldrich (St. Louis, MO, USA). Ultrapure water (18.2 MΩ/cm) was purified by a Milli-Q purification system (Millipore, Billerica, MA, USA), and nitrogen (99.999%) was obtained from AGA industrial gases (Lidingö, Sweden).
Stock solutions of 1000 mg/L were prepared for each standard in methanol. Linear calibration curves were prepared in the range of 2-100 mg/L, except for anthocyanins which were prepared in the range of 2-200 mg/L.
Stock solutions of 1000 mg/L were prepared for each standard in methanol. Linear calibration curves were prepared in the range of 2-100 mg/L, except for anthocyanins which were prepared in the range of 2-200 mg/L.
6
LC-MS Solvent Preparation Protocol
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LC–MS grade methanol (MeOH), water, and additives (ammonium formate and formic acid) were purchased from Sigma Aldrich, Gillingham, UK.
7
Acquisition of LC-MS Reagents
8
Quantitative Analysis of Drugs and Metabolites
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Individual solutions of 1 mg mL−1 of AMP, MAMP, MDMA, COC, benzoylecgonine (BE), 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH), cotinine (COT) and trans-3′-hydroxycotinine (OH-COT), and 0.1 mg mL−1 of their deuterated analogues (AMP-D6, MAMP-D5, MDMA-D5, COC-D3, BE-D3, THC-COOH-D9, COT-D3 and OH-COT-D3) used as surrogate internal standards (ISs), were supplied by Cerilliant (Round Rock, TX, USA). Sodium salts of Ethyl-Sulfate (EtS) and its deuterated analogue (EtS-D5) were supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA). Individual solutions of 1 mg mL−1 of the S-(+) enantiomer of AMP, MAMP and MDMA were supplied by Merck (Darmstadt, Germany).
LC-MS grade methanol (MeOH), LC-MS grade acetic acid, LC-MS grade formic acid, ammonia (NH3) solution in water (25%), tetrabutylammonium bromide (TBA, 99%) and β-Glucuronidase (from Helix pomatia, type H2) were supplied by Merck. Sodium acetate and sodium chloride were obtained from Fluka (Steinheim, Germany). Ultrapure water was obtained with a Genie Water System from RephiLe Bioscience (Boston, MA, USA).
LC-MS grade methanol (MeOH), LC-MS grade acetic acid, LC-MS grade formic acid, ammonia (NH3) solution in water (25%), tetrabutylammonium bromide (TBA, 99%) and β-Glucuronidase (from Helix pomatia, type H2) were supplied by Merck. Sodium acetate and sodium chloride were obtained from Fluka (Steinheim, Germany). Ultrapure water was obtained with a Genie Water System from RephiLe Bioscience (Boston, MA, USA).
9
Synthesis and Characterization of Phenylethylpiperidine Standards
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Reference standards of phenylethylpiperidine compounds were synthesized in-house at Janssen R&D (Beerse, Belgium). 2-Fluorobenzyl alcohol, 3-fluorobenzyl alcohol and 4-fluorobenzyl alcohol standards were obtained from Sigma-Aldrich (St Louis, USA). LC-MS grade methanol (MeOH) and water (H 2 O) were obtained from Merck (Darmstadt, Germany). Cesium chloride salt was obtained from Sigma-Aldrich (St Louis, USA).
10
Formic Acid Preparation and Purification
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Formic acid (FA) was purchased from Sigma-Aldrich (Saint Louis, MO, United States). Ultra-pure H2O was prepared by a Milli-Q Pure Water System (Bedford, MA, United States). LC-MS grade methanol (MeOH) and acetonitrile (ACN) were purchased from Merck (Darmstadt, Germany).
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