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Pgl6 mir based luciferase reporter vector

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The PGL6-miR-based luciferase reporter vector is a plasmid-based system used for the quantitative analysis of microRNA (miRNA) activity. It contains a luciferase reporter gene that is regulated by a miRNA-responsive element, allowing for the measurement of miRNA-mediated repression of gene expression.

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Lab products found in correlation

Dual luciferase reporter assay system, by Beyotime (1 mentions)

2 protocols using pgl6 mir based luciferase reporter vector

1

HMGB3 3′-UTR Luciferase Assay

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The wild-type or mutant 3′-untranslated region (UTR) of HMGB3 was inserted into the pGL6-miR‐based luciferase reporter vector (Beyotime). SW480 cells were co-transfected with HMGB3 wild-type or mutant plasmids and the miR-200b-3p agomir or agomir NC using Lipofectamine 2000 for 48 h. Next, the luciferase activity was detected with the Dual Luciferase Reporter Assay System.
Yuan H., Chen B., Chai R., Gong W., Wan Z., Zheng B., Hu X., Guo Y., Gao S., Dai Q., Yu P, & Tu S. (2022). Loss of exosomal micro-RNA-200b-3p from hypoxia cancer-associated fibroblasts reduces sensitivity to 5-flourouracil in colorectal cancer through targeting high-mobility group box 3. Frontiers in Oncology, 12, 920131.
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2

Luciferase Reporter Assay for miR-216b-5p

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The wild-type (wt) or mutant (mut) miR-216b-5p binding sites to SNHG1 or 3ʹ-UTR of JAK2 were separately inserted into the pGL6-miR‐based luciferase reporter vector (Beyotime), then co-transfected with miR-216b-5p mimics and NC mimic into cells. After 48 h of transfection, the luciferase activity was detected using the Dual Luciferase Reporter Assay System (Beyotime).
Dai G., Yang Y., Liu S, & Liu H. (2022). Hypoxic Breast Cancer Cell-Derived Exosomal SNHG1 Promotes Breast Cancer Growth and Angiogenesis via Regulating miR-216b-5p/JAK2 Axis. Cancer Management and Research, 14, 123-133.
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