Anti dsrna antibody

Inhibitors of lipid biosynthesis were obtained from Cayman Chemical; EquiSPLASH lipidomics internal standard was obtained from Avanti Polar Lipids. Anti-dsRNA antibody was obtained from Millipore (identifier MABE1134); anti-mouse IgG AlexaFluor 647 was obtained from Invitrogen (Identifier A32628); anti-llama secondary HRP, goat IgG was obtained from Novus (identifier NB7242). 5 mM BSA-palmitate complex was obtained from Cayman Chemical (identifier 29558); fatty acid-free BSA was obtained from Thermo Fisher (identifier BP9704100).

Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), phosphate buffer saline (PBS), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M), L-glutamine (100X), non-essential amino acids (100X), and antibiotics (Pen, Strep, Amphotericin B, 100X) were purchased from Thermo Fisher Scientific (Gibco^TM, Waltham, MA, USA). All the cell culture dishes were purchased from Corning. Microscopic glass cover slips were purchased from Blue Star. Molecular grade paraformaldehyde, triton X-100, and 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY) were purchased from Thermo Fisher Scientific. ZORBAX RRHD Eclipse Plus C18 column with 1.8 µ particle size, 2.1 mm inner diameter, and length 100 mm were purchased from Agilent Technologies, Santa Clara, CA, USA. Anti-actin and anti-HCV core antibodies were purchased from BIO-RAD (Hercules, CA, USA) and GeneTex (Irvine, CA, USA) respectively. Anti-dsRNA antibody was purchased from Millipore (Merk-Sigma, Burlington, MA, USA).

Anti-dsRNA antibody (Clone: rJ2,) was purchased from Sigma (Cat# MABE1134) or Antibodies online (Cat# Ab01299–23.0). Polyclonal SARS-CoV-2 RNA-dependent RNA Polymerase antibody was purchased from CellSignaling (Cat# 67988S). Monoclonal SARS-CoV-2 RNA-dependent RNA Polymerase antibody was purchased from Kerafest (Cat# ESG004). Anti-Spike (Spike 1) antibody (clone: 1A9, Cat# GTX632604) was obtained from GeneTex. Anti-Spike (Spike 2) antibody (clone: T01Khu, Cat# 703958) was obtained from ThermoFisher.