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2 protocols using sodium citrate phr1416

1

Investigating Cytokine-Mediated Signaling

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Recombinant human IL-2 (200–02) and mouse IL-7 (217–17) were from Peprotech. IAA (I2515), CoCl2 (232696), sodium citrate (PHR1416), and UK5099 (PZ0160) were from Sigma-Aldrich. PX-478 2HCl (px478, S7612) was from Selleck.
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2

Comprehensive Cellular Protein Analysis

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Antibody: β-actin (Genetex, Irvine, CA, USA, GTX109639), CPT1A (Abcam, Cambridge, UK, ab128568), PARP1 (Abcam, ab32138), PAR (Trevigen, 4335-MC-100, 4336-BPC-100), rH2AX (Merck Millipore, Bedford, MA, USA, 05-636 and Cell Signaling Technology, 2577 s), Flag M2 (Sigma-Aldrich, F1804), Lys-Ac (Cell Signaling Technology, 9441), 53BP1 (Cell Signaling Technology, 4937 s), BRCA1 (Santa Cruz Biotechnology, California, USA, sc-6954), AMPK (Cell Signaling Technology, 5831 s), p-AMPK (Cell Signaling Technology, 2535 s), Alexa 594 (Invitrogen, A11012), FITC (Santa Cruz Biotechnology, sc-516140), Alexa 488 (Invitrogen, A11029), Alexa Fluor™ 488 Phalloidin (Thermo Fisher Scientific, A12379), Mouse IgG-HRP (Genetex, GTX213111-01), and Rabbit IgG-HRP (Genetex, GTX213110-01). Trichostatin A (T8552), Sodium Butyrate (B5887), Etomoxir (E1905), Etoposide (E1383), Doxorubicin (D1515), C646 (SML0002), Octanoate (C5038), Sodium Acetate (S5636), and Sodium Citrate (PHR1416) were purchased from Sigma-Aldrich. PDD00017273 (Selleckchem Chemicals, Houston, Texas, USA, S8862) and Nocodazole (S2775) were purchased from Selleckchem.
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