Anti phospho mkk3

Anti-phospho-JNK, anti-JNK, anti-phospho-p38, anti-p38, anti-phospho-MKK3, anti-phospho-MKK4, anti-phospho-ATF2, anti-phospho-MK2, anti-phospho-CREB, anti-phospho-cjun, anti-phospho-IκBɑ, anti-phospho-STAT1, anti-phospho-STAT3, anti-IL-1β, anti-cleaved caspase-3, and anti-phospho-ASK1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-ASK1 antibody was provided from Dr. Hidenori Ichijo. Anti-F4/80 antibody was obtained from Serotec. Anti-CD4, anti-caspase-1, and anti-Dclk1 antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-TFF2 antibody was kindly provided by Dr. Sachiyo Nomura. Anti-proton pump and anti-Cdx2 antibodies were obtained from Santa Cruz (Santa Cruz, CA, USA). Anti-BrdU antibody was obtained from Dako (Santa Clara, CA, USA). Anti-actin antibody was purchased from Sigma. Anti-cyclin D1(AB3) was acquired from Invitrogen (Waltham, MA, USA).

2‐Phenylbenzimidazole‐5‐sulphonic acid (PBSA) was obtained from Sigma‐Aldrich. The structure of PBSA is presented in Figure 1A. The following chemical agents and antibodies were purchased from commercial sources: p38^MAPK inhibitor, SB203580 (Cayman Chemical); anti‐phospho‐Src (Y416), anti‐Src, anti‐phospho‐ERK (T202/Y204), anti‐phospho‐Akt (S473), anti‐phospho‐p70^S6K (T421/S424), anti‐phospho‐MKK3 (S189)/MKK‐6 (S207), anti‐MKK3, anti‐MKK6, anti‐phospho‐p38^MAPK (T180/Y182), anti‐phospho‐pRb (S780), anti‐phospho‐pRb (S807/S811), anti‐MMP‐2 and anti‐MMP‐9 (Cell Signaling); anti‐phospho‐FAK (Y397) and anti‐FAK (BD Biosciences); anti‐ERK, anti‐Akt, anti‐p70^S6K, anti‐38^MAPK, anti‐TIMP‐2, anti‐Cdk4, ant‐Cdk2, anti‐cyclin D, anti‐cyclin E, anti‐integrin β1, anti‐EGFR, anti‐FGFR‐1, anti‐VEGFR‐2, anti‐actin and mouse and rabbit IgG‐horseradish peroxidase conjugates (Santa Cruz Biotechnology Inc).