Milli q advantage a10 system apparatus

Manufactured by Merck Group

Sourced in Italy

The Milli-Q Advantage A10 System is a water purification apparatus designed to produce high-purity water. It utilizes a multi-stage filtration process to remove various contaminants, resulting in ultrapure water suitable for laboratory and research applications.

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Lab products found in correlation

Methanol, by Merck Group (6 mentions) Phosphoric acid, by Merck Group (4 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (3 mentions) Lc ms grade acetonitrile, by Merck Group (3 mentions) Ethanol, by Merck Group (3 mentions) Acetonitrile, by Merck Group (3 mentions) Apigenin, by Extrasynthese (2 mentions) Luteolin, by Extrasynthese (2 mentions) Luteolin 7 o glucoside, by Extrasynthese (2 mentions) Ferric chloride, by Merck Group (2 mentions) Gallic acid, by Merck Group (2 mentions) Sodium carbonate, by Merck Group (2 mentions) Formic acid, by Merck Group (2 mentions) Ferrous sulphate, by Merck Group (1 mentions) Copper chloride dihydrate, by Merck Group (1 mentions) Potassium persulphate, by Merck Group (1 mentions) Copper sulphate pentahydrate, by Merck Group (1 mentions) Neocuproine, by Merck Group (1 mentions) Folin ciocalteu s reagent, by Merck Group (1 mentions) Ammonium acetate, by Merck Group (1 mentions)

7 protocols using milli q advantage a10 system apparatus

Antioxidant and Cytotoxic Evaluation of Natural Oils

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Soy lecithin and neem oil were purchased from Galeno srl (Carmignano, Prato, Italy). Sodium hyaluronate was purchased by DSM Nutritional Products AG Branch Pentapharm (Dornacherstrasse 112 CH-4147 Aesch BL/Switzerland). Argan oil, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), gallic acid, ferrous sulphate, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), (±)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tris(2-pyridyl)-1,3,5-triazine (TPTZ), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), neocuproine (2,9-dimethyl-1,10-phenanthroline) hydrochloride, Folin-Ciocalteu’s reagent, sodium carbonate, ferric chloride, ammonium acetate, copper chloride dihydrate, potassium persulphate, copper sulphate pentahydrate and all the other reagents were of analytical grade and were purchased from Sigma-Aldrich (Milan, Italy). Ultrapure water (18 MΩ·cm) was obtained with a Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy). Reagents and plastics for cell culture were purchased from Life Technologies Europe (Monza, Italy).

Manca M.L., Manconi M., Meloni M.C., Marongiu F., Allaw M., Usach I., Peris J.E., Escribano-Ferrer E., Tuberoso C.I., Gutierrez G., Matos M, & Ghavam M. (2021). Nanotechnology for Natural Medicine: Formulation of Neem Oil Loaded Phospholipid Vesicles Modified with Argan Oil as a Strategy to Protect the Skin from Oxidative Stress and Promote Wound Healing. Antioxidants, 10(5), 670.

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Phospholipid-Based Antioxidant Extraction

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Phospholipon90G (P90G; phosphatidylcholine content ≥ 94%) was purchased from Lipoid GmbH (Ludwigshafen, Germany). Propylene glycol (PG) were purchased from Galeno Srl (Comeana, Prato, Italy). LC-MS grade acetonitrile, formic acid, methanol, 85% w/w phosphoric acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tris(pyridin-2-yl)-1,3,5-triazine (TPTZ), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), dimethyl sulphoxide (DMSO), and all the other reagents, if not otherwise specified, were purchased from Sigma-Aldrich/Merck (Milan, Italy). Standards of 5-O-caffeoylquinic (chlorogenic acid), 1,3-dicaffeoylquinic acid (cynarin), 3,5-dicaffeoylquinic acid (isochlorogenic acid A), 3,4-dicaffeoylquinic acid (isochlorogenic acid B), 4,5-dicaffeoylquinic acid (isochlorogenic acid C), ferulic acid, apigenin, hispidulin, and luteolin were purchased from Extrasynthese (Genay, France) and TransMIT (Giessen, Germany). Ultrapure water (18 MΩ·cm) was obtained with a Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy).

Caddeo C., Tuberoso C.I., Floris S., Masala V., Sanna C, & Pintus F. (2023). A Nanotechnological Approach to Exploit and Enhance the Bioactivity of an Extract from Onopordum illyricum L. Leaves. Plants, 12(7), 1453.

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Analytical-Grade Solvents for Phenolic Compounds

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All the solvents and chemicals used in this study were of analytical grade. M ethanol, ethanol, acetonitrile LC-MS grade, and 85% phosphoric acid were purchased from Merck (Darmstadt, Germany, ethanol from Carlo Erba Reagenti (Milan, Italy). Standards of phenolic compounds were obtained from Extrasynthese (Genay, France), TransMIT (Giessen, Germany), and Merck-Sigma-Aldrich (Milan, Italy). Ultrapure water (18 MΩ·cm) was obtained with a Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy).

Spissu Y., Gil K.A., Dore A., Sanna G., Palmieri G., Sanna A., Cossu M., Belhadj F., Gharbi B., Pinna M.B., Barberis A., Tuberoso C.I, & D’hallewin G. (2022). Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells. Antioxidants, 12(1), 80.

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Phospholipid-Based Antioxidant Formulation

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Lipoid S75 (S75), a mixture of soybean phospholipids (~70% phosphatidylcholine, 9% phosphatidylethanolamine and 3% lysophosphatidylcholine), triglycerides and fatty acids, was purchased from AVG S.r.l. (Garbagnate Milanese, Milan, Italy), a local supplier for Lipoid GmbH (Ludwigshafen, Germany). Sodium hyaluronate was purchased from DSM Nutritional Products AG Branch Pentapharm (Aesch—BL/Switzerland). Tween 80, 2,2-diphenyl-1-picrylhydrazyl (DPPH), tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), ethanol, acetonitrile, methanol, phosphoric acid, and all the other solvents and reagents of analytical grade were purchased from Sigma-Aldrich (Milan, Italy). Standards of luteolin-7-O-glucoside, luteolin, apigenin, chlorogenic acid and verbascoside were purchased from Extrasynthese (Genay Cedex, France).
Reagents and plastics for cell culture were purchased from Life Technologies Europe (Monza, Italia). Ultrapure water (18 MΩ·cm) was obtained using a Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy).

Firoznezhad M., Castangia I., Tuberoso C.I., Cottiglia F., Marongiu F., Porceddu M., Usach I., Escribano-Ferrer E., Manca M.L, & Manconi M. (2022). Formulation and In Vitro Efficacy Assessment of Teucrium marum Extract Loading Hyalurosomes Enriched with Tween 80 and Glycerol. Nanomaterials, 12(7), 1096.

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Quantitative Analysis of Plant Compounds

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All the used chemicals were of analytical grade. Standard of homogentistic, acid (±)-2-cis, 4-trans-abscisic acid, kurarinone, sakuranetin, and quinine sulphate were purchased from Sigma–Aldrich (Milan, Italy). (±)-2-trans, 4-trans-abscisic acid was purchased from A.G. Scientific, Inc (San Diego, CA, USA). Unedone was purchased from Chem Faces Biochemical Co., Ltd. (Wuhan, China). Methanol, acetonitrile, phosphoric acid 85% w/w, and absolute ethanol were obtained from Sigma–Aldrich (Milan, Italy). acetonitrile, water, and formic acid of LC-MS grade were purchased from Merck (Darmstadt, Germany). Ultrapure water (18 MΩ cm) was obtained with Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy).

Montoro P., D’Urso G., Kowalczyk A, & Tuberoso C.I. (2021). LC-ESI/LTQ-Orbitrap-MS Based Metabolomics in Evaluation of Bitter Taste of Arbutus unedo Honey. Molecules, 26(9), 2765.

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Extraction and Characterization of Soybean Phospholipids

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Lipoid S75 (fat-free soybean phospholipids with 70% phosphatidylcholine; S75) was supplied by Lipoid GmbH (Ludwigshafen, Germany). Standards of phenolic compounds (cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, malvidin-3-O-glucoside, peonidin-3-O-glucoside, petunidin-3-O-glucoside, gallic acid, ellagic acid, myricetin-3-O-galactoside, and myricetin-3-O-rhamnoside) were purchased from Extrasynthese (Genay Cedex, France). Ultrapure water (18 MΩ cm) was obtained with a Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy). Acetonitrile for ultra-phase liquid chromatography (UPLC, gradient grade) analyses, methanol, phosphoric acid, phosphate buffered saline (PBS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tris(pyridin-2-yl)-1,3,5-triazine (TPTZ), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and other reagents were purchased from Sigma-Aldrich/Merck (Milan, Italy), unless otherwise specified.

De Luca M., Lucchesi D., Tuberoso C.I., Fernàndez-Busquets X., Vassallo A., Martelli G., Fadda A.M., Pucci L, & Caddeo C. (2022). Liposomal Formulations to Improve Antioxidant Power of Myrtle Berry Extract for Potential Skin Application. Pharmaceutics, 14(5), 910.

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Phytochemical Characterization and Antioxidant Evaluation

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All the solvents and chemicals used in this study were analytical grade. M ethanol, ethanol, acetonitrile LC-MS grade, formic acid LC-MS grade, 85% phosphoric acid, and water LC-MS grade were purchased from Merck^® (Darmstadt, Germany). Gallic acid, quinic acid, caffeic acid, ferulic acid, protocatechuic acid, neo chlorogenic acid (3-O-caffeoylquinic acid), chlorogenic acid (5-O-caffeoylquinic acid), cynarin (1,3-dicaffeoylquinic acid), isochlorogenic acid, kaempferol-3-O-glucoside, quercetin-3-O-glucoside, ferrous sulfate, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH^●), (±)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tris(2-pyridyl) -1,3,5-triazine (TPTZ), 2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), Folin–Ciocalteu reagent, sodium carbonate, ferric chloride, AlCl₃, NaNO₂, and CuSO₄∙5H₂O were purchased from Merck^®, Sigma-Aldrich^®, or Fluka™ (Milan, Italy). Flavonoid standards of apigenin, apigenin-7-O-glucoside, hesperidin, hesperetin, luteolin-7-O-glucoside, naringin, and naringenin were purchased from Extrasynthese (Genay, France). Ultrapure water (18 MΩ·cm) was obtained with a Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy).

Bouzabata A., Montoro P., Gil K.A., Piacente S., Youssef F.S., Al Musayeib N.M., Cordell G.A., Ashour M.L, & Tuberoso C.I. (2022). HR-LC-ESI-Orbitrap-MS-Based Metabolic Profiling Coupled with Chemometrics for the Discrimination of Different Echinops spinosus Organs and Evaluation of Their Antioxidant Activity. Antioxidants, 11(3), 453.

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