2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS, 94398, Sigma), Ferrous Sulphate Heptahydrate (F8263, Sigma), γ-Aminobutyric acid (GABA, A2129, Sigma), 4-Hydroxy-2,5-dimethyl-3(2H)-furanone (W317403, Sigma).
Ferrous sulphate heptahydrate
Manufactured by Merck Group
Sourced in Germany, United States
Ferrous sulphate heptahydrate is a chemical compound with the formula FeSO4·7H2O. It is a crystalline solid that appears green in color. The compound is used as a source of iron in various applications.
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Lab products found in correlation
Ferric chloride hexahydrate, by Merck Group (11 mentions)
Sodium hydroxide (naoh), by Merck Group (6 mentions)
Ammonium hydroxide, by Merck Group (4 mentions)
Sodium nitrite, by Merck Group (3 mentions)
Methanol, by Merck Group (3 mentions)
Amphotericin b, by Merck Group (2 mentions)
Glacial acetic acid, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Anhydrous sodium carbonate, by Merck Group (2 mentions)
Folin ciocalteu reagent, by Merck Group (2 mentions)
Liquid ammonia, by Merck Group (2 mentions)
Ammonia solution, by Merck Group (2 mentions)
Styrene, by Merck Group (2 mentions)
Zinc chloride, by Merck Group (1 mentions)
Multi element calibration standard, by Agilent Technologies (1 mentions)
Dicyclohexylcarbodiimide, by Merck Group (1 mentions)
Metronidazole, by Merck Group (1 mentions)
Aluminium chloride, by Merck Group (1 mentions)
Sodium acetate trihydrate, by Merck Group (1 mentions)
Sodium hydroxide solution, by Merck Group (1 mentions)
24 protocols using ferrous sulphate heptahydrate
1
Quorum Sensing Molecule Extraction
2
Preparation and Characterization of Metal-Based Compounds
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Ferric chloride hexahydrate (FeCl3·6H2O), ferrous sulphate heptahydrate (FeSO4·7H2O), zinc chloride (ZnCl2), cerium (III) nitrate hexahydrate (Ce(NO3)3·6H2O), and sodium hydroxide (NaOH) were purchased from Sigma-Aldrich Merck (Darmstadt, Germany) and used as such.
For the ICP-MS analysis, calibration solutions were prepared from multi-element calibration standards purchased from Agilent Technologies (Santa Clara, CA, USA, p.n: 8500-6944 and 8500-6940). The sample preparation was conducted using trace analysis grade nitric acid (HNO3) purchased from Honeywell (Charlotte, NC, USA), while all dilutions were performed with Milli-Q water (18 MΩ·cm).
The Baby Hamster Kidney fibroblasts (BHK) and human hepatocarcinoma (HepG2) cell lines were provided by the Institute for Diagnosis and Animal Health (I.D.A.H.).
The microbial strains (S. aureus ATCC 25923, P. aeruginosa ATCC 15442, and C. albicans ATCC 10231) used for the antimicrobial assays were provided by the Microbiology and Immunology Department from the Faculty of Biology, University of Bucharest.
For the ICP-MS analysis, calibration solutions were prepared from multi-element calibration standards purchased from Agilent Technologies (Santa Clara, CA, USA, p.n: 8500-6944 and 8500-6940). The sample preparation was conducted using trace analysis grade nitric acid (HNO3) purchased from Honeywell (Charlotte, NC, USA), while all dilutions were performed with Milli-Q water (18 MΩ·cm).
The Baby Hamster Kidney fibroblasts (BHK) and human hepatocarcinoma (HepG2) cell lines were provided by the Institute for Diagnosis and Animal Health (I.D.A.H.).
The microbial strains (S. aureus ATCC 25923, P. aeruginosa ATCC 15442, and C. albicans ATCC 10231) used for the antimicrobial assays were provided by the Microbiology and Immunology Department from the Faculty of Biology, University of Bucharest.
3
Synthesis and Characterization of Magnetic Nanoparticles
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All organic solvents utilized in experiments were of high-performance liquid chromatography (HPLC) grade and purchased form Fisher scientific UK. Ferric Sulphatehexahydrate Fe2(SO4)3·6H2O, Ferrous sulphateheptahydrate (FeSO4·7H2O), 3-(Trimethoxysilyl)propyl methacrylate (MPTES), 4-dimethyl amino pyridine (DMAP), azobisisobutyronitrile (AIBN), ammonium hydroxide, dicyclohexylcarbodiimide (DCC), metronidazole and amphotericin B were obtained from Sigma-Aldrich, Merck Darmstadt (Germany) through a local supplier.
4
Comprehensive Phytochemical Characterization
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Folin-Ciocalteu reagent, gallic acid monohydrate, methanol, hydrochloric acid, ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), potassium persulfate (K2S2O8), Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), aluminium chloride (AlCl3), sodium nitrite (NaNO2), sodium hydroxide solution (NaOH), quercetin, sodium acetate trihydrate (CH3COONa·3H2O), glacial acetic acid (CH3COOH), 2,4,6-Tripyridyl-s-Triazine (TPTZ), ferric chloride (FeCl3), and ferrous sulphate heptahydrate (FeSO4·7H2O) were supplied from Sigma-Aldrich (Milan, Italy). Anhydrous sodium carbonate (Na2CO3) was supplied from Carlo Erba (Milan, Italy). For the preparation of the phosphate buffered saline (PBS), the following salts were used: sodium phosphate dibasic heptahydrate (HNa2O4P·7H2O) and sodium phosphate monobasic monohydrate (H2NaO4P·H2O). These were purchased from Sigma-Aldrich (Milan, Italy). All reagents were of analytical grade.
5
Recombinant Protein Expression in E. coli
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Chemically competent Escherichia coli BL21(DE3)T1R (CMC0014), CelLytic™ B Cell Lysis Reagent, ferrous sulphate heptahydrate, KOH and other compounds were supplied by Sigma-Aldrich (St. Louis, Missouri, USA). GFP (FPbase: TurboGFP; GenBank: ASW25889), sialic acid aldolase (SAA, UniProt: P0A6L4; NCBI-GeneID: 947742) and UDP–glucose pyrophosphorylase (GalU, UniProt: P0AEP3; NCBI-GeneID: 945730) genes were N-terminally fused with the cellulose-binding domain from Clostridium cellulovorans by cloning into plasmid pET-34b (Additional file 1 ).
6
Synthesis of Iron-Containing Compounds
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DL-Lys monohydrochloride (98%), Gly (99%), DL-Trp (99%), ferric chloride hexahydrate (FeCl3·6H2O), ferrous sulphate heptahydrate (FeSO4·7H2O), ammonium hydroxide (NH4OH), and perchloric acid (HClO4) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
7
Synthesis of Functionalized Silica Nanoparticles
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Ferrous sulphate heptahydrate (FeSO4.7H2O), potassium hydroxide (KOH), tetraethylorthosilicate (TEOS), potassium nitrate (KNO3), aminopropyltriethoxysilane (APTES), sodium hydroxide (NaOH) and diglycolic anhydride were purchased from Sigma Aldrich. Phosphoric acid, tetrahydrofuran (THF), acetic acid, ethanol, toluene, lead nitrate, boric acid and potassium dichromate were purchased from other commercial suppliers. All reagents were of analytical grade and were used without further purification. Solvents were freshly dried according to standard protocols for the synthetic work.
8
Biocompatible and Antimicrobial Assays
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Ferric chloride hexahydrate (FeCl3·6H2O), ferrous sulphate heptahydrate (FeSO4·7H2O), sodium hydroxide (NaOH), streptomycin sulfate, and neomycin trisulfate were purchased from Sigma-Aldrich Merck (Darmstadt, Germany) and used as acquired.
The biocompatibility assay involved the use of normal BJ human dermal fibroblast cells (CLS, Heidelberg, Germany).
For the antimicrobial assays, three microbial strains were used (a Gram-negative bacterial species, i.e., Pseudomonas aeruginosa ATCC 27853, a Gram-positive bacterial species, i.e., Staphylococcus aureus ATCC 25923, and a fungal species, i.e., Candida albicans ATCC 10231), which were obtained from the Faculty of Biology, University of Bucharest.
The biocompatibility assay involved the use of normal BJ human dermal fibroblast cells (CLS, Heidelberg, Germany).
For the antimicrobial assays, three microbial strains were used (a Gram-negative bacterial species, i.e., Pseudomonas aeruginosa ATCC 27853, a Gram-positive bacterial species, i.e., Staphylococcus aureus ATCC 25923, and a fungal species, i.e., Candida albicans ATCC 10231), which were obtained from the Faculty of Biology, University of Bucharest.
9
Catecholamine and Iron Chelator Assay
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The catecholamines epinephrine (EPI, Figure 1(a) ) and ISO (Figure 1(a) ), copper chelator D-PA (Figure 1(c) ), ferric chloride hexahydrate (FeCl3·6H2O), ferrous sulphate heptahydrate (FeSO4·7H2O), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulphonic acid sodium salt (ferrozine), sodium acetate, acetic acid, 4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid (HEPES), HEPES sodium salt, hydroxylamine, salicylic acid, and 2,3-dihydroxybenzoic and 2,5-dihydroxybenzoic acids were purchased from Sigma-Aldrich (USA). Deferoxamine was purchased from Novartis (Switzerland), dimethyl sulfoxide (DMSO) was purchased from Avantor Performance Materials (USA), and methanol for HPLC was purchased from JT Baker (USA). The chemicals and solutions used for cellular cultivation (cultivation media, sera, etc.) were purchased from Sigma-Aldrich or Lonza Group (Switzerland).
10
Synthesis and Characterization of Functionalized Magnetic Nanoparticles
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All organic solvents utilized in experiments were of HPLC grade. Ferric sulphate hexahydrate Fe2(SO4)3.6H2O, ferrous sulphate heptahydrate (FeSO4.6H2O), 3-(trimethoxysilyl)propyl methacrylate (MPTES), 4-dimethyl amino pyridine (DMAP), azobisisobutyronitrile (AIBN), ammonium hydroxide, dicyclohexylcarbodimide (DCC), isoniazid and amphotericin B were obtained from Sigma-Aldrich (San Francisco, CA, USA) and Merck (Darmstadt, Germany).
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