N nitro l arginine methyl ester

To snap-suppress NO bioactivity under sodium restriction, the subgroup of DCM mice on LSD was randomly assigned for L-NAME (N-nitro-L-arginine methyl ester; Sigma, St. Louis, MO, USA), potent inhibitor of NO synthase (NOS), treatment by a single tail vein slow-infusion of 200 µL over 1 min at a concentration (1 mg/kg in saline, at 37 °C) that does not modulate blood pressure, but influences cGMP production as reported [40 (link)]. Mice were euthanized 4 h after L-NAME infusion; blood was collected for examination.

Lead acetate Pb (CH₃COO)₂, L-phenylephrine hydrochloride, acetylcholine chloride, sodium nitroprusside, N-nitro-L arginine methyl ester, catalase, superoxide dismutase, diethyldithiocarbamic acid, apocynin and tetraethylammonium were purchased from Sigma-Aldrich (Sigma Chemical Co., USA). The salts and reagents used were of analytical grade and were from Sigma-Aldrich and Merck (Darmstadt, Germany).

Acetylcholine chloride, angiotensin II, indomethacin, N-nitro-L-arginine methyl ester (L-NAME), 2-(Methylthio) adenosine 5′-trihydrogen diphosphatetrisodium (2-MeS-ADP), MRS-2395 [2,2-Dimethyl-propionic acid 3-(2-chloro-6-methylaminopurin-9-yl)-2-(2,2-dimethyl-propionyloxymethyl)-propyl ester], sodium nitroprusside (SNP) and phenylephrine hydrochloride, were purchased from Sigma Aldrich (St. Louis, MO). Clopidogrel bisulfate (Bristol-Myers Squibb Company, New York, NY, USA) was obtained by crushing Plavix tablets (75 mg). MRS-2179 tetrasodium salt [2′-Deoxy-N6-methyladenosine 3′,5′-bisphosphate tetrasodium salt] and MRS-2211 2 disodium salt ([(2-Chloro-5-nitrophenyl)azo]-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]-4-pyridinecarboxaldehyde) were purchased from Tocris (Ellisville, MO).