Anti atrx

Manufactured by Abcam

Anti-ATRX is a primary antibody that recognizes the ATRX protein. ATRX is a chromatin remodeling protein involved in regulating gene expression. The antibody can be used in various applications, including Western blotting, immunohistochemistry, and immunofluorescence, to detect and study the ATRX protein.

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Lab products found in correlation

Anti h3, by Merck Group (1 mentions) Anti h3k27me3, by Merck Group (1 mentions) Anti pcna, by Santa Cruz Biotechnology (1 mentions) Anti 53bp1, by Novus Biologicals (1 mentions) Telomere pna fish kit cy3, by Agilent Technologies (1 mentions) Anti rad50, by Abcam (1 mentions) Anti atrx, by Santa Cruz Biotechnology (1 mentions) Anti nbs1, by BD (1 mentions) Anti rpa32 s33, by Fortis Life Sciences (1 mentions)

2 protocols using anti atrx

ATRX Chromatin Immunoprecipitation Protocol

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Neuro2A and Neuro2A-ATRXKO cells were grown in DMEM for 48 hours, collected in 10mL DMEM and crosslinked in 1% formaldehyde. The cells were lysed in SDS buffer and sonicated. Immunoprecipitation was performed with the following antibodies: anti-ATRX (Abcam, ab97508), anti-H3K27me3 (Millipore, 07-449), anti-H3.3 (Millipore, CS207327). Rabbit IgG (Cell Signaling) was used as a control. Input samples represent 1/25 of total chromatin input. Real-time PCR experiments were conducted as follows: 95°C for 5 min, 40 cycles of 95°C for 10 s, 60°C for 20 s, and 72°C for 30 s. This procedure was repeated in triplicate. Primers used to amplify the P1-P4 regions are listed in Table S4.

Tamming R.J., Dumeaux V., Jiang Y., Shafiq S., Langlois L., Ellegood J., Qiu L.R., Lerch J.P, & Bérubé N.G. (2020). Atrx Deletion in Neurons Leads to Sexually Dimorphic Dysregulation of miR-137 and Spatial Learning and Memory Deficits. Cell Reports, 31(13), 107838.

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Immunofluorescence Staining of DNA Damage Proteins

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Cells grown on coverslips were prepared for IF by standard procedures. The following antibodies were used for immunostaining: anti-ATRX (Santa Cruz sc-15408); anti-ATRX 39f [38] (link); anti MRE11 (Abcam ab214); anti-MRE11 (Calbiochem PC388); anti-RAD50 (Abcam ab89); anti-PCNA (Santa Cruz sc-9857); anti-RPA32 (S33) (Bethyl A300-246A); anti-NBS1 (BD Biosciences 611871); anti-53BP1 (Novus Biologicals NB100-305); For MRN co-localisation studies cells were pre-permeabilised prior to fixation with ice cold 0.5% Triton X-100. For MRE11/ATRX/PCNA co-localisation in mES cells, cells were attached to coverslips via poly-l-lysine treatment prior to pre-permeabilisation. Telomere FISH was performed subsequent to antibody incubation using Telomere PNA FISH Kit/Cy3 (Dako K5326).

Clynes D., Jelinska C., Xella B., Ayyub H., Taylor S., Mitson M., Bachrati C.Z., Higgs D.R, & Gibbons R.J. (2014). ATRX Dysfunction Induces Replication Defects in Primary Mouse Cells. PLoS ONE, 9(3), e92915.

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