CCA cell lines HuCCT1 and HuH28 were purchased from Japan Health Science Research Resources Bank, and CCA cell lines SNU-1196, SNU-1079, SNU-308, SNU-245, SNU-478 and SNU-869 were purchased from Korea Cell Line Bank. Human HEK293T was obtained from American Type Culture Collection (ATCC). All cell lines were cultured with RPMI-1640 medium (Invitrogen, USA) supplemented with 10% fetal bovine serum (Hyclone, USA) and 1% PenStrep (100 U/mL Penicilium and 100 μg/mL Streptomycin) in a humid atmosphere containing 5% CO2 at 37 °C. Gemcitabine resistant cell lines HuCCT1-Gem and SNU-245-Gem were constructed by exposing to increasing dosages of Gemcitabine (Selleck Chemicals, USA) for 8 months, and then persistently cultured in medium containing 10 nmol/l Gemcitabine.
Snu1079
Manufactured by Korean Cell Line Bank
Sourced in Japan
The SNU1079 is a cell line product offered by the Korean Cell Line Bank. It is a laboratory equipment used for culturing and maintaining cells in a controlled environment. The SNU1079 cell line provides a standardized and reliable source of cells for research and experimentation purposes.
Automatically generated - may contain errors
Lab products found in correlation
Snu245, by Korean Cell Line Bank (11 mentions)
Snu1196, by Korean Cell Line Bank (10 mentions)
Snu 308, by Korean Cell Line Bank (9 mentions)
Snu478, by Korean Cell Line Bank (9 mentions)
Snu869, by Korean Cell Line Bank (8 mentions)
Tfk 1, by RIKEN BioResource Center (4 mentions)
Hucct1, by Japanese Collection of Research Bioresources Cell Bank (3 mentions)
Hucct1, by RIKEN BioResource Center (3 mentions)
Trcn0000196945, by Merck Group (2 mentions)
Kku m055, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Kku m213, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Mmnk 1, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Human hek293t, by American Type Culture Collection (1 mentions)
Gemcitabine, by Selleck Chemicals (1 mentions)
Hucct1, by Health Science Research Resources Bank (1 mentions)
Huh28, by Health Science Research Resources Bank (1 mentions)
Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
14 protocols using snu1079
1
Establishment of Gemcitabine-Resistant CCA Cell Lines
2
Characterization of Biliary Tract Cancer Cell Lines
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Twenty human biliary tract cancer cell lines were collected from different sources. Huh-28 (ICC), HuCCT1 (ICC), OZ (CPHBD), NOZ (CGB) and OCUG-1 (CGB) cell lines were obtained from the Japanese Collection of Research Bioresources Cell Bank. TKKK (ICC), TFK-1 (CPHBD), TGBC1TKB (CGB), TGBC2 (CGB), TGBC14TKB (CGB), TGBC24TKB (CGB) and G-415 (CGB) cell lines were obtained from the RIKEN BioResource Center. SNU-1079 (ICC), SNU-245 (CPHBD), SNU-1196 (CPHBD) and SNU-308 (CGB) cell lines were obtained from the Korean Cell Line Bank. The Egi-1 (CPHBD) cell line was obtained from the Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures. All cell lines were cultured as recommended by their respective cell banks. SK-ChA-1 (CPHBD), Mz-ChA-1 (CGB), Mz-ChA-2 (CGB) were obtained from Prof. Alexander Knuth (University Hospital of Zürich, Zürich, Switzerland) (32 (link)) and cultured in RPMI 1640 with 10 mM HEPES, 2 mM L-Glutamine, 1X MEM non-essential amino acids (Thermo Fisher Scientific), 100 U/ml penicillin, 100 µg/ml of streptomycin, and 10% fetal bovine serum (FBS). Human BMSCs (196hT) immortalized with the hTERT/GFP system (33 (link)) were cultured as described previously (34 (link)). The control cell line 293/hTNW that stably expresses human tenascin-W (27 (link)) was cultured in DMEM with 0.25 µg/ml of G418, 1.5 µg/ml of puromycin, and 10% FBS.
3
Cell Culture of Human CCA Lines
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4
BTC Cell Lines and Chemotherapeutics
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Nine human BTC cell lines were utilized in this research. The SNU245, SNU308, SNU478, SNU869, SNU1079, and SNU1196 cell lines were purchased from Korean Cell Line Bank (Seoul, Korea). The HuCCT-1 and TFK-1 cell lines were obtained from RIKEN BioResource Center (Ibaraki, Japan). A patient-derived cell line, SNU2670, was successfully established [19 (link)]. All cells were cultured in RPMI-1640 medium (Welgene Inc., Gyeongsan, Korea) containing 10% fetal bovine serum and 10 μg/mL gentamicin at 37°C incubator under 5% CO2. The ATR inhibitor AZD6738 was kindly provided by AstraZeneca (Macclesfield, Cheshire, UK). Gemcitabine, cisplatin, and 5-fluorouracil (5-FU) were purchased from Lilly Korea Co. (Seoul, Korea), JW Pharmaceutical Co. (Seoul, Korea), and Ildong Pharmaceutical Co. (Seoul, Korea), respectively.
5
Cultivating Biliary Tract Cancer Cell Lines
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Five human biliary tract cancer cell lines (SNU-245, SNU-308, SNU-478, SNU-1079, and SNU-1196) were procured from the Korea Cell Line Bank (Seoul, Korea). Cell lines were cultured in Rosewell Park Memorial Institute 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Cells maintained in 5% CO2-humidified atmosphere at 37℃.
6
Establishment of Human BTC Cell Lines
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A total of 11 human BTC cell lines were used in this study. SNU-245, SNU-308, SNU-478, SNU-869, SNU-1079, SNU-1196, and SNU-2823 cell lines were obtained from Korean Cell Line Bank, Seoul, Korea. HuCCT1 and TFK-1 cell lines were obtained from RIKEN BioResource Center, Ibaraki, Japan. HER2-amplified human BTC cell lines, SNU-2670 and SNU-2773, were established from tumor tissues from BTC patients using a previously described method [31 (link)].
All the cell lines were maintained in RPMI-1640 culture media supplemented with 10% fetal bovine serum (WELGENE Inc., Gyeongsan, Korea) and 10 μg/mL gentamicin in a humidified atmosphere containing 5% CO2 at 37°C.
All the cell lines were maintained in RPMI-1640 culture media supplemented with 10% fetal bovine serum (WELGENE Inc., Gyeongsan, Korea) and 10 μg/mL gentamicin in a humidified atmosphere containing 5% CO2 at 37°C.
7
Culturing Cholangiocarcinoma Cell Lines
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The HuCCT1 cell line was purchased from the Health Science Research Resources Bank (Osaka, Japan), whereas other cholangiocarcinoma cell lines (SNU245, SNU308, SNU478, SNU869, SNU1079, and SNU1196) were purchased from the Korean Cell Line Bank (Seoul, Korea). The cell lines were grown in RPMI1640 supplemented with 10% fetal bovine serum (FBS), 25mM HEPES, and 100 μg/ml of penicillin/streptomycin in a 5% CO2 atmosphere at 37°C.
8
Modulation of AXL and GAS6 in Cholangiocarcinoma Cell Lines
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KKU-M055, KKU-M213, MMNK1 and HUCCT1 cells were purchased from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan); and SNU245, SNU478, SNU869, SNU1079 and SNU1196 cells were purchased from the Korean Cell Line Bank (KCLB; Seoul, Republic of Korea). All cell lines were maintained in their specified medium supplemented with 10% FBS (SERANA) at 37 °C in a 95% air and 5% CO2 incubator. AXL shRNA in lentiviral was purchased and transfected into HEK293T cells (Sigma Aldrich #TRCN0000196945, 5′-GATTGCCATTGAGAGTCTAGC-3′). Cell media were collected 48 h after and filtered using a 0.45 µm syringe filter to collect viral particles. SNU1196 and HUCCT1 cells (2 × 105 cells/well) were plated into a 96-well plate 24 h before incubation with media containing viral particles overnight at 37 °C. The media were removed and replaced with fresh culture media, and puromycin (2 mg/mL) was used to select stable AXL knockdown cells, which was confirmed by Western blotting. For GAS6 stimulation, cells were kept in serum-free medium for 24 h and then treated with 10 μg/mL or 100 μg/mL AVB-500 with the addition of 50 ng/mL GAS6 (R&D systems #885-GSB-050) for 24 h.
9
Cell Culture Conditions for Analysis
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SNU1079 was purchased from the Korean Cell Line Bank, CX004T2 was produced in house, and the other CCA cell lines were purchased from the RIKEN Cell Bank. All cell lines were cultured in high glucose DMEM (Corning, 10-017-CV) containing 10% (v/v) FBS (Thermo Fisher Scientific, 26140079) and 1% (v/v) penicillin/streptomycin (Thermo Fisher Scientific, 15-140-122) in an incubator at 37°C with 5% CO2. A list of all inhibitors and antibodies used is provided in Supplemental Table 1 .
10
Establishment of AXL Knockdown Cell Lines
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