Micro slides

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Micro Slides are flat, rectangular glass or plastic plates used for the examination and analysis of small samples, typically in a laboratory setting. They provide a stable and controlled surface for the placement and observation of specimens under a microscope.

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Lab products found in correlation

Lindberg blue m, by Thermo Fisher Scientific (4 mentions) Chicken anti gfp, by Thermo Fisher Scientific (2 mentions) Alexa 488 anti chicken, by Thermo Fisher Scientific (2 mentions) Vectashield hart set mounting medium with dapi, by Vector Laboratories (2 mentions) Lubriseal, by Thomas Scientific (2 mentions) Bx 61 light microscope, by Leica (2 mentions) Facsaria, by BD (2 mentions) Negative selection kit, by STEMCELL (2 mentions) Macsquant analyzer 10, by Miltenyi Biotec (2 mentions) Sonifier 450, by Emerson (1 mentions) Weighing balance, by Ohaus (1 mentions) Rabbit anti rfp, by Rockland Immunochemicals (1 mentions) 3 trimethoxysilyl propyl methacrylate, by Merck Group (1 mentions) Irgacure 2959, by BASF (1 mentions) Rbc lysis buffer, by Thermo Fisher Scientific (1 mentions) Percoll, by Merck Group (1 mentions) Tcs sp5 confocal laser scanning microscope, by Leica (1 mentions) Sprague dawley rats sd, by Charles River Laboratories (1 mentions) Cm3050 cryostat, by Avantor (1 mentions) Specific mouse anti α actinin monoclonal antibody, by Merck Group (1 mentions)

Spelling variants of this product

Superfrost Plus Micro Slides (33 citations) Superfrost Plus glass micro slides (2 citations) Micro glass cover slides (2 citations) VWR Micro Slides (2 citations) Superfrost micro slides (2 citations) Superfrost Plus VWR Micro Slides (2 citations)

20 protocols using micro slides

Quantifying MRSA Biofilm Dry Mass

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Dry mass
is a widely used maker and quick method to quantify biofilm growth,
and it is usually expressed as mass per unit area or biofilm density.
This experiment was performed according to a previously reported method
with slight modifications.^{82 (link)} MRSA biofilms
were formed on standard glass microscope slides (Micro slides; VWR
Scientific, Inc., West Chester, PA, USA) for 7 days. The biofilms
were washed three times by gently swirling in physiological saline
to detach nonadherent bacteria and treated with 100× MIC of the
hydrogel samples using a sterile Pasteur pipette. The biofilms were
transferred into sterile saline solution, and the adherent cells were
harvested from the glass surfaces by gently scraping with a sterile
spatula. The scraped biofilms were sonicated (three 10 s pulses with
5 s intervals at 50 W) using Branson Sonifier 450. Three volumes of
cold ethanol were added to 5 mL of the cell suspension; the supernatant
was discarded. The resulting precipitate was lyophilized and weighed
using a weighing balance (Ohaus corp. Pine Brook, NJ, USA).

Fasiku V.O., Omolo C.A., Devnarain N., Ibrahim U.H., Rambharose S., Faya M., Mocktar C., Singh S.D, & Govender T. (2021). Chitosan-Based Hydrogel for the Dual Delivery of Antimicrobial Agents Against Bacterial Methicillin-Resistant Staphylococcus aureus Biofilm-Infected Wounds. ACS Omega, 6(34), 21994-22010.

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Immunohistochemical Analysis of Brain Samples

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Immunohistochemistry follows protocols previously reported^{15 (link),16 ,18 (link)}. Mice were overdosed with 3% isoflurane and perfused transcardially with cold (4 °C) phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (PFA) in PBS. Brains were extracted and stored overnight in PFA at 4 °C. Fifty μm coronal sections were collected in serial order using a vibratome and collected in cold PBS. Sections were blocked for 1 hour at room temperature in PBST and 5% normal goat serum (NGS) or Bovine albumin serum (BSA) on a shaker. Sections were transferred to wells containing primary antibodies (1:1000 guinea anti-c-Fos [SySy]; 1:1000 rabbit anti-RFP [Rockland]; 1:5000 chicken anti-GFP [Invitrogen]) and allowed to incubate on a shaker overnight at room temperature or 3 days at 4 degrees C. Sections were then washed in PBST for 10-min (x3), followed by 2-hour incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa anti-guinea 647 [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen]). Following three additional 10-min washes in PBST, sections were mounted onto micro slides (VWR International, LLC). Vectashield Hart Set Mounting Medium with DAPI (Vector Laboratories, Inc) was applied, slides were coverslipped, and allowed to dry overnight.

Shpokayte M., McKissick O., Guan X., Yuan B., Rahsepar B., Fernandez F.R., Ruesch E., Grella S.L., White J.A., Liu X.S, & Ramirez S. (2022). Hippocampal cells segregate positive and negative engrams. Communications Biology, 5, 1009.

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Annealing Graphene Films for Characterization

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Example 17

Annealing Study of Graphene Films.

An ink containing graphene/ethyl cellulose in ethanol/terpineol was prepared for blade-coating films. Graphene/ethyl cellulose powder (˜100 mg) was dispersed in 2 mL of 4:1 ethanol/terpineol v/v by bath sonication. This ink was blade-coated onto glass slides (VWR Micro Slides) into a 15×15 mm²film defined by a mask of scotch tape. The sample was then annealed in a tube furnace (Thermo Scientific, Lindberg Blue M). The sheet resistance of the resulting film was measured by a 4-point probe technique, employing the appropriate geometric correction factors, while the film thickness was measured by profilometry (Dektak 150 Stylus Surface Profiler). These results were used to calculate the resistivity plotted in FIG. 13A-B.

US09902866B2. Methods for preparation of concentrated graphene ink compositions and related composite materials (2018-02-27). Northwestern University [US], President and Fellows of Harvard College [US]. Inventors: Mark C. Hersam [US], Yu Teng Liang [US], Ethan B. Secor [US], Pradyumna L. Prabhumirashi [US], Kanan P. Puntambekar [US], Michael L. Geier [US], Bok Y. Ahn [US], Jennifer A. Lewis [US].

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Comprehensive Cytospin Analysis of Blood and Ascites Cells

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Cytospins were prepared using sort-purified cells from PBMCs and ascites cells, micro slides (VWR, 48311–703) and a Shandon Cytospin2 Centrifuge. A Wright-Giemsa staining protocol was used. Slides were fixed with methanol (1 min), residual methanol was decanted, and slides were stained with a modified-Wright stain (Sigma-WS-128, 0.3%, buffered at pH 6.9 in methanol, contains stabilizer and surfactant) (5 min). Three milliliter layers of freshly prepared modified-Giemsa stain (Sigma-GS-128, 0.4% in buffered methanol solution pH 6.8 with stabilizers) were placed onto the slides for 30 minutes. Afterwards, slides were rinsed with tap water, air dried, rinsed with methanol and washed under running tap water, dried and then cover slips were placed. Toluidine blue staining was performed to confirm magenta-colored staining of basophil granules. Morphologic evaluation was performed by a hematopathologist (JTW).

Khan A.N., Emmons T.R., Wong J.T., Alqassim E., Singel K.L., Mark J., Smith B.E., Tario J.D., Eng K.H., Moysich K.B., Odunsi K., Abrams S.I, & Segal B.H. (2020). Quantification of Early-Stage Myeloid-Derived Suppressor Cells in Cancer Requires Excluding Basophils. Cancer immunology research, 8(6), 819-828.

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PEG Hydrogel Preparation and Glass Silanization

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PEG hydrogel solutions were prepared by combining 10–20% w/v poly(ethylene glycol) diacrylate (PEG-DA, 3350 MW, 94.45% acrylation, ESI BIO) with 0.05%–0.10% w/v Irgacure 2959 photoinitiator (2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone, I2959, BASF) in deionized water (diH₂O) or 1× S-basal buffer (100 mM NaCl, 50 mM KPO₄ buffer, pH 6.0). A clean 1″ × 3″ glass slide (VWR Micro Slides) was rendered permanently hydrophobic byexposure to vapors of (tridecafluoro-1,1,2,2-tetrahydrooctyl) trichlorosilane (Gelest) under vacuum for 1 h, or temporarily hydrophobic by wiping with Rain-X Glass Water Repellent. Glass slides were cleaned with ethanol and water, then dried by air gun. For covalent attachment of hydrogels to glass, #1.5 cover slips (Thermo Scientific) were silanized by coating with 3-(trimethoxysilyl)propyl methacrylate (Sigma-Aldrich), 21 mM in ethanol for 3 min, followed by ethanol wash, water rinse, and air dry. Treated glass slides can be prepared months in advance. Spacers were prepared by casting polydimethylsiloxane (PDMS, Sylgard 184, Ellsworth Adhesives) in a 1:10 (curing agent:base) ratio to thicknesses of 100, 200, and 500 µm.

Burnett K., Edsinger E, & Albrecht D.R. (2018). Rapid and gentle hydrogel encapsulation of living organisms enables long-term microscopy over multiple hours. Communications Biology, 1, 73.

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Volumetric Pollen Monitoring Methodology

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Intact pollen grain concentrations were measured using a volumetric spore trap (Burkard Manufacturing Company) co-located with other measurement platforms. The samples were collected on to a microscopic slide (75 × 25 × 1.0 mm, Micro Slides, VWR) coated with a thin layer of grease (Lubriseal, Thomas Scientific). The collected samples were mounted and imaged using Olympus BX-61 light microscope with an automated slide scanner (Leica Aperio Ariol). All pollen taxa are included in the intact pollen grain concentrations. Longitudinal counting method was used to obtain 1 h pollen counts. Birch pollen identification was conducted for May 8, 16, 17, 18, and 24 by re-analyzing higher resolution images of 1 h intervals starting at 15:00, 22:00 and 5:00 to differentiate birch/mulberry pollen grains from previous publication (Hughes et al., 2020 (link)). Further description of slide mounting, instrumental analysis, pollen identification and determination of pollen counts are detailed in the supporting information of Hughes et al. (2020) (link).

Mampage C.B., Hughes D.D., Jones L.M., Metwali N., Thorne P.S, & Stone E.A. (2022). Characterization of sub-pollen particles in size-resolved atmospheric aerosol using chemical tracers. Atmospheric Environment: X, 15, 100177.

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Isolation and Analysis of Immune Cells

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BM cells were flushed, and a single-cell suspension was made by passing through the syringe/needles. Cells from spleen and lymph nodes were prepared by gently grinding the dissected organs with micro slides (VWR, Radnor, PA). Blood was drawn from the cheeks and mixed with 3.8% sodium citrate (Ricca Chemical Company, Batesville, IN). Red blood cells were lysed by RBC lysis buffer (Thermo-Fisher Scientific, Waltham, MA). For liver lymphocytes acquisition, 10 mL PBS was injected into a hepatic artery to perfuse the blood from the liver. After dissecting and grinding the liver, lymphocytes were separate through Percoll (Sigma, St. Louis, MO) gradient centrifugation (40% and 60%). Flow cytometry analyses were conducted in LSR-II (BD Biosciences, San Jose, CA) or MACSQuant Analyzer 10 (Miltenyi Biotec, Bergisch Gladbach, Germany) and analyzed with FlowJo software (FlowJo LLC, Ashland, OR). For cell sorting, NK cells were first enriched using negative selection kit (STEMCELL Technologies, Vancouver, Canada). The specific subsets of NK cells were further sorted by FACSAria (BD Biosciences, San Jose, CA), and the purity was generally above 95%.

Yang C., Tsaih S.W., Lemke A., Flister M.J., Thakar M.S, & Malarkannan S. (2018). mTORC1 and mTORC2 differentially promote natural killer cell development. eLife, 7, e35619.

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Tracking Implanted hMSCs in Rat Heart

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To confirm the delivery of hMSCs with microthreads, two Sprague Dawley rats (SD, Charles River Laboratories), weighing 500-600 g, were sacrificed 1 hour and 1 week after implantation of Qdot655 labeled hMSC seeded fibrin microthreads. Heart tissues were immediately frozen in liquid N₂ and then placed in an embedding mold and immersed in OCT compound and immediately placed in a −80°C freezer. Once the OCT compound was completely frozen, the block was placed in a Leica CM3050 cryostat and 6 μm thick heart sections were cut and placed on VWR® MicroSlides. Cryosections were fixed in ice cold acetone for 10 min, and then nonspecific epitope antigens blocked with goat serum at room temperature for 60 min. The sections were incubated with specific mouse anti-α-actinin monoclonal antibody (Sigma, Catalog No A7811) at room temperature for 1 hour, and treated with goat anti-mouse secondary antibody (Alexa Fluor 488 A11029) at room temperature for 1 hour. The nuclei were stained with Hoechst33342 (0.5 μg/ml) for 5 min at room temperature. Fluorescent images were obtained with a Leica TCS SP5 confocal laser scanning microscope.

Tao Z.W., Favreau J.T., Guyette J.P., Hansen K.J., Lessard J., Burford E., Pins G.D, & Gaudette G.R. (2014). Delivering Stem Cells to the Healthy Heart on Biological Sutures: Effects on Regional Mechanical Function. Journal of tissue engineering and regenerative medicine, 11(1), 220-230.

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Visualization of RPE Cell Polarization

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The morphologic features of polarization were visualized by immunolocalization of ZO-1.20 (link),21 (link) Highly differentiated polarized hRPE (TER = 380 ± 60 Ω·cm²) were treated with either 15 mM PS or 15 mM BM with or without cotreatment with 2 mM GSH-MEE. As described earlier,21 (link) RPE monolayers were fixed and blocked before incubation with ZO-1 rabbit polyclonal antibody (Table) at 4°C overnight, and followed by incubation with FITC conjugated anti-rabbit secondary antibody (Vector Laboratories) for 30 minutes. After the immunostaining procedure, membranes were cut, mounted on micro slides (VWR) and viewed on a laser-scanning microscope (Carl Zeiss Microscopy).

Wang M., Lau L.I., Sreekumar P.G., Spee C., Hinton D.R., Sadda S.R, & Kannan R. (2019). Characterization and Regulation of Carrier Proteins of Mitochondrial Glutathione Uptake in Human Retinal Pigment Epithelium Cells. Investigative Ophthalmology & Visual Science, 60(2), 500-516.

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Annealing Study of Graphene Films

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Example 17

Annealing Study of Graphene Films.

US10590294B2. Methods for preparation of concentrated graphene ink compositions and related composite materials (2020-03-17). Northwestern University [US], President and Fellows of Harvard College [US]. Inventors: Mark C. Hersam [US], Yu Teng Liang [US], Ethan B. Secor [US], Pradyumna L. Prabhumirashi [US], Kanan P. Puntambekar [US], Michael L. Geier [US], Bok Y. Ahn [US], Jennifer A. Lewis [US].

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