Fluorescence microscopy was performed using a DMIRB inverted microscope (Leica Microsystems), with a cooled charge-coupled device camera (Princeton Instruments). Images were collected with a 20× objective. To perform ‘prismless’ TIRF microscopy a 60×1.45 numerical aperture oil-immersion objective (Olympus America) was used. The evanescent field decay length was 100–250 nm. MetaMorph software (Universal Imaging) was used for image processing and quantification as previously described [35 (link)].
1.45 numerical aperture oil immersion objective
Manufactured by Olympus
The 60x/1.45 numerical aperture oil-immersion objective is a high-magnification lens designed for use in microscopy applications. This objective provides a high level of optical resolution and light-gathering capability, making it suitable for detailed examination of small samples. The numerical aperture of 1.45 indicates the lens's ability to collect a large cone of light, resulting in improved image quality and resolution.
Automatically generated - may contain errors
2 protocols using 1.45 numerical aperture oil immersion objective
1
Prismless TIRF Microscopy for Imaging
2
High-Resolution TIRF Microscopy Protocol
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