Log In Sign up
The largest database of trusted experimental protocols

Anti cd64 r pe clone 10

Manufactured by Agilent Technologies
Visit Supplier

Anti-CD64 R-PE (clone 10.1) is a fluorescently-labeled antibody that binds to the CD64 protein. CD64 is a high-affinity Fc receptor expressed on the surface of certain immune cells. This product can be used for the identification and enumeration of CD64-positive cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Blue viability dye, by Thermo Fisher Scientific (2 mentions) Anti cd3 alexa fluor 700 clone, by Agilent Technologies (2 mentions) Anti cd56 pe cy7, by Agilent Technologies (2 mentions) Anti cd64 fitc clone 10, by Agilent Technologies (2 mentions) Anti cd89 pe clone a, by Agilent Technologies (2 mentions) Anti cd45 pe cy5 clone, by Agilent Technologies (2 mentions) Anti cd15 pacificblue clone, by Agilent Technologies (2 mentions) Anti cd16 bv510 clone, by Agilent Technologies (2 mentions) Anti cd32 apc clone fun 2, by Agilent Technologies (2 mentions) Perm a buffer, by Thermo Fisher Scientific (2 mentions) Anti cd68 fitc clone kp1, by Agilent Technologies (2 mentions) Perm b, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (2 mentions)

2 protocols using anti cd64 r pe clone 10

1

Comprehensive Fc Receptor Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometric evaluation of Fc receptor expression was performed on freshly isolated cells from enzymatically digested colon and cervical tissues. 1x106 colon or 1x105 cervical cells were stained in two panels with anti-CD3 Alexa Fluor 700 (clone UCHT1), anti-CD56 PE-Cy7 (clone NCAM16.2), anti-CD64 FITC (clone 10.1), anti-CD89 PE (clone A59), anti-CD45 PE-Cy5 (clone HI30), anti-CD15 PacificBlue (clone W6D3), anti-CD16 BV510 (clone 3G8), anti-CD32 APC (clone FUN-2), anti-CD64 R-PE (clone 10.1, Dako), blue viability dye (Life Technologies). Cells were washed and fixed with Perm A buffer (Life Technologies). Intracellular staining with anti-CD68 FITC (clone KP1, Dako) was performed in the presence of permeabilization buffer (Perm B, Life Technologies). The data was acquired using an LSRII flow cytometer (BD Biosciences) and analyzed using FlowJo and SPICE version 5.1.
Sips M., Krykbaeva M., Diefenbach T.J., Ghebremichael M., Bowman B.A., Dugast A.S., Boesch A.W., Streeck H., Kwon D.S., Ackerman M.E., Suscovich T.J., Brouckaert P., Schacker T.W, & Alter G. (2016). Fc Receptor-Mediated Phagocytosis in Tissues as a Potent Mechanism for Preventive and Therapeutic HIV Vaccine Strategies. Mucosal immunology, 9(6), 1584-1595.
+ Open protocol
+ Expand
2

Comprehensive Fc Receptor Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometric evaluation of Fc receptor expression was performed on freshly isolated cells from enzymatically digested colon and cervical tissues. 1x106 colon or 1x105 cervical cells were stained in two panels with anti-CD3 Alexa Fluor 700 (clone UCHT1), anti-CD56 PE-Cy7 (clone NCAM16.2), anti-CD64 FITC (clone 10.1), anti-CD89 PE (clone A59), anti-CD45 PE-Cy5 (clone HI30), anti-CD15 PacificBlue (clone W6D3), anti-CD16 BV510 (clone 3G8), anti-CD32 APC (clone FUN-2), anti-CD64 R-PE (clone 10.1, Dako), blue viability dye (Life Technologies). Cells were washed and fixed with Perm A buffer (Life Technologies). Intracellular staining with anti-CD68 FITC (clone KP1, Dako) was performed in the presence of permeabilization buffer (Perm B, Life Technologies). The data was acquired using an LSRII flow cytometer (BD Biosciences) and analyzed using FlowJo and SPICE version 5.1.
Sips M., Krykbaeva M., Diefenbach T.J., Ghebremichael M., Bowman B.A., Dugast A.S., Boesch A.W., Streeck H., Kwon D.S., Ackerman M.E., Suscovich T.J., Brouckaert P., Schacker T.W, & Alter G. (2016). Fc Receptor-Mediated Phagocytosis in Tissues as a Potent Mechanism for Preventive and Therapeutic HIV Vaccine Strategies. Mucosal immunology, 9(6), 1584-1595.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!