Embelin

Embelin was purchased from Tocris Bioscience (Ellisville, MO). MTT was purchased from Sigma (St. Louis MO, MA). LY294002 and zVAD-fmk was purchased from Calbiochem (San Diego, CA, USA). XIAP antibody was purchased from BD Transduction lab (San Jose, CA, USA). Antibodies against caspase-9, caspase-3, PARP, p-AKT, p-Bad, Bcl-2, Bcl-Xl, Beta-actin, Survivin and Bid were purchased from Cell Signaling Technologies (Beverly, MA, USA). Cytochrome c and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). cIAP-1 antibody was purchased from R&D (USA). Annexin V/PI staining kit was purchased from Molecular Probes (Eugene OR, USA).

Embelin was purchased from Tocris Bioscience (Minneapolis, MN). zVAD-fmk was purchased from Calbiochem (San Diego, CA). Antibodies against caspase-9, caspase-8, Bid, Bcl-xL, phospho AKT and cleaved caspase-3, caspase-3 were purchased from Cell Signaling Technologies (Beverly, MA). Cytochrome c, PARP and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). XIAP antibody was purchased from Abcam (Cambridge, England). Annexin V-FITC, Propidium iodide staining solution, Hoechst 33342 Solution, BD Cytofix/Cytoperm plus fixation and permeabilization solution kit, PE Active Caspase-3 Apoptosis Kit and Apoptosis, BD MitoScreen (JC-1) Kit, DNA Damage and Cell Proliferation Kit were purchased from BD Biosciences (NJ, USA). AKT, XIAP and Scrambled control siRNA were purchased from Qiagen (Valencia, CA, USA). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA). 5-(2-Benzothiazolyl)-3-ethyl-2-[2-(methylphenylamino)ethenyl]-1-phenyl-1H-benzimidazolium iodide was purchased from Sigma Aldrich (USA).

Olaparib was purchased from Selleck Chemicals (Houston, TX). Embelin was purchased from Tocris Bioscience (Ellisville, MO). Antibodies against PARP, caspase-8, caspase-9, caspase-3, Bcl-2, Bcl-xl and cIAP1 were purchased from Cell Signaling Technologies (Beverly, MA). XIAP antibody was purchased from BD Pharmingen (San Diego, CA, USA). Survivin, Cytochrome c and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Annexin V was purchased from Thermo Fischer Scientific (Waltham, MA). caspase-8 inhibitor, z-IETD-FMK was purchased from R&D Systems (Minneapolis, MN, USA).