Np19 ova

Manufactured by Biosearch Technologies

Sourced in United States

NP19-OVA is a type of nanoparticle product developed by Biosearch Technologies. It is designed for use in various research and development applications. The core function of NP19-OVA is to serve as a versatile tool for researchers, without further interpretation or extrapolation on its intended use.

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Lab products found in correlation

Imject alum, by Thermo Fisher Scientific (2 mentions) Ovalbumin (ova), by Biosearch Technologies (2 mentions) Complete freund s adjuvant, by Merck Group (2 mentions) 5 ethynyl 2 deoxyuridine (edu), by Merck Group (2 mentions) Monophosphoryl lipid a, by Merck Group (1 mentions) Np ficoll, by Biosearch Technologies (1 mentions) Np46 ficoll, by Biosearch Technologies (1 mentions) Complete freund adjuvant (cfa), by Merck Group (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions) Pc bsa, by Biosearch Technologies (1 mentions) Np26 bsa, by Biosearch Technologies (1 mentions) Indomethacin, by Merck Group (1 mentions)

6 protocols using np19 ova

Antigen-Specific T Cell Activation Protocol

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Mice were immunized intraperitoneally (i.p.) with 50 μg endotoxin‐free ovalbumin (Endograde OVA; Profos) in combination with monophosphoryl lipid A (Sigma‐Aldrich) or Imject alum (Thermo Scientific) as adjuvants. For analysis of affinity maturation, OT‐II‐cell transferred WT or Cd3e^–/– mice were immunized i.p. with 50 μg NP₁₉‐OVA (Biosearch Technologies) in alum. For SW_HEL and OT‐II co‐transfers, mice were immunized i.p. with 30–35 μg of HEL^WT‐OVA_323‐339 or the mutated form HEL^2X‐OVA_323‐339 in alum (recombinant proteins were kindly provided by Humabs BioMed).

Preite S., Baumjohann D., Foglierini M., Basso C., Ronchi F., Rodriguez B.M., Corti D., Lanzavecchia A, & Sallusto F. (2015). Somatic mutations and affinity maturation are impaired by excessive numbers of T follicular helper cells and restored by Treg cells or memory T cells. European Journal of Immunology, 45(11), 3010-3021.

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Immunization with NP-Ficoll and NP-OVA

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Mice were immunized with NP-Ficoll (Biosearch Technologies, Middleton, WI, USA, 10 μg/100 μL/mouse in PBS) or NP₁₉-OVA (Biosearch Technologies) adsorbed onto Imject alum (Thermo Scientific, Waltham, MA, USA, 1:1, 30 μg/100 μL/mouse) intraperitoneally.

Zhao Y., Zhao S., Qin X.Y., He T.T., Hu M.M., Gong Z., Wang H.M., Gong F.Y., Gao X.M, & Wang J. (2022). Altered Phenotype and Enhanced Antibody-Producing Ability of Peripheral B Cells in Mice with Cd19-Driven Cre Expression. Cells, 11(4), 700.

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Preimmunization and Adoptive Transfer Protocol

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For B1-8^hi transfer experiments, C57BL/6 recipient mice were preimmunized by i.p. injection of 100 µg OVA (BioSearch Technologies) dissolved in PBS and precipitated in alum (Sigma-Aldrich) at a 1:1 ratio >2 wk before adoptive cell transfer. 12–24 h after B1-8^hi cell transfer, recipient mice were immunized by i.p. injection of 100 µg NP₁₉-OVA (BioSearch Technologies) precipitated in alum (Sigma-Aldrich) at a 1:1 ratio. For NP-Ficoll immunization, non-preimmunized C57BL/6 recipient mice were i.p. injected with 100 µg NP₄₆-Ficoll (BioSearch Technologies) dissolved in PBS and precipitated in alum at a 1:1 ratio. For in vivo proliferation analysis, 1 mg EdU (Sigma-Aldrich) diluted in PBS was i.p. or i.v. injected at indicated time points after immunization. For PE immunization, mice were s.c. injected with 50 µl of an emulsion containing 50 µg of PE. Emulsions were prepared by mixing PE in PBS in a 1:1 ratio with complete Freund’s adjuvant (Sigma-Aldrich). For peptide immunizations, mice were i.p. injected with 100 µg Ag85b_280-294 (FQDAYNAAGGHNAVF) or 2W1S (EAWGALANWAVDSA) peptide in 100 µl of a 1:1 emulsion with complete Freund’s adjuvant.

Rauschmeier R., Reinhardt A., Gustafsson C., Glaros V., Artemov A.V., Dunst J., Taneja R., Adameyko I., Månsson R., Busslinger M, & Kreslavsky T. (2021). Bhlhe40 function in activated B and TFH cells restrains the GC reaction and prevents lymphomagenesis. The Journal of Experimental Medicine, 219(2), e20211406.

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Investigating T Cell-Mediated Immune Responses

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The mice transferred with splenic CD4 T cells (1 × 10⁶ cells) from OTII Tg Tgm2^+/+ or Tgm2^−/− mice were intraperitoneally injected with 100 μg NP₁₉-OVA (Biosearch Technologies) in CFA (Sigma) next day after cell transfer. Serum samples were collected 1 and 3 weeks after the immunization. The concentrations of NP-specific Igs (IgG1 and IgG2c) were determined by ELISA (48 (link)). Alternatively, Tgm2^fl/flCd4Cre and control Tgm2^fl/fl mice were intraperitoneally injected with 100 μg whole OVA (Sigma) plus 10 μg LPS (Invivogen) in 200 μl PBS at an interval of 2 weeks. Serum samples were collected and analyzed 4 weeks after the last immunization. To detect in vivo generated memory Th cells, whole splenocytes were stimulated with OVA (100 μg /mL) for 4 h in the presence of 2 μM monensin, and detected CD154⁺ and IFNγ⁺ CD4T cells by intracellular staining (49 (link)).

Suzuki A.S., Yagi R., Kimura M.Y., Iwamura C., Shinoda K., Onodera A., Hirahara K., Tumes D.J., Koyama-Nasu R., Iismaa S.E., Graham R.M., Motohashi S, & Nakayama T. (2020). Essential Role for CD30-Transglutaminase 2 Axis in Memory Th1 and Th17 Cell Generation. Frontiers in Immunology, 11, 1536.

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Ovalbumin-based Immune Stimulation Protocol

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cOVA (“Imject OVA”) was purchased from Thermo Scientific (Rockford, IL). (NP)₁₉-OVA, (NP)₂₆-BSA and PC-BSA were obtained from Biosearch Technologies (Novato, CA). Alum (Allhydrogel 2%) was obtained from Brenntag Biosector (Denmark). Indomethacin was obtained from Sigma (St. Louis, MO). PC covalently linked to keyhole limpet hemocyanin (KLH) and truncated (first 302 amino acids) recombinant PspA was made as previously described (19 (link)).

S.a.u.m.y.a.a., Pujanauski L., Colino J., Flora M., Torres R.M., Tuomanen E, & Snapper C.M. (2016). Pneumococcal surface protein A plays a major role in Streptococcus pneumoniae-induced immunosuppression. Journal of immunology (Baltimore, Md. : 1950), 196(9), 3677-3685.

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Pre-immunization and Adoptive Transfer Protocol

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For B1-8 hi transfer experiments, C57BL/6 recipient mice were pre-immunized by intraperitoneal injection of 100 µg OVA (BioSearch Technologies) dissolved in PBS and precipitated in alum (Sigma-Aldrich) at a 1:1 (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. ratio > 2 weeks prior to adoptive cell transfer. 12 to 24 hours after B1-8 hi cell transfer, recipient mice were immunized by intraperitoneal injection of 100 µg NP19-OVA (BioSearch Technologies) precipitated in alum (Sigma-Aldrich) at a 1:1 ratio. For in vivo proliferation analysis, 1 mg 5-Ethynyl-2′-deoxyuridine (EdU, Sigma-Aldrich) diluted in PBS was intraperitoneally or intravenously injected at indicated time-points post immunization. For Phycoerythrin (PE) immunization, mice were subcutaneously injected with 50 μl of an emulsion containing 50 μg of PE. Emulsions were prepared by mixing PE in PBS in a 1:1 ratio with Complete Freund's Adjuvant (Sigma-Aldrich). For peptide immunizations, mice were intraperitoneally injected with 100 µg Ag85b280-294 (FQDAYNAAGGHNAVF) or 2W1S (EAWGALANWAVDSA) peptide in 100 µl of a 1:1 emulsion with Complete Freund's Adjuvant (Sigma-Aldrich).

Rauschmeier R., Reinhardt A., Gustafsson C., Glaros V., Artemov A.V., Taneja R., Adameyko I., Månsson R., Busslinger M., & Kreslavsky T. (2021). Cell-intrinsic functions of the transcription factor Bhlhe40 in activated B cells and T follicular helper cells restrain the germinal center reaction and prevent lymphomagenesis.

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