RNA was isolated from spleen tissue with the RNA Mini Kit (Qiagen, Hilden, Germany). Quantitation of RNA was performed with a NanoDrop ND‐1000 spectrophotometer (Thermo Scientific). The RNA was reverse‐transcribed to cDNA with the Quantitect Reverse Transcription Kit (Qiagen). Gene expression analysis was performed with GAPDH, Stat1, Irf7, Oas1, Mx1, Ifnα4, Ifnβ1, and Usp18 assays (Qiagen). For analysis, the expression levels of all target genes were normalized against GAPDH (dCt). Gene expression values were then calculated by the delta‐delta‐Ct (ddCt) method, with the mean of the control group as the calibrator to which all other samples were compared. Relative quantities (RQs) were determined with the equation RQ = 2−ddCt.
Usp18
Manufactured by Qiagen
Sourced in Germany
Usp18 is a laboratory equipment product manufactured by Qiagen. It is a protein that plays a key role in the regulation of the immune system. Usp18 functions as a deubiquitinase, which removes ubiquitin modifications from target proteins, thereby modulating their activity and stability.
Automatically generated - may contain errors
Lab products found in correlation
Ifnβ1, by Qiagen (2 mentions)
Rna mini kit, by Qiagen (1 mentions)
Nanodrop nd 1000, by Thermo Fisher Scientific (1 mentions)
Ifnα4, by Qiagen (1 mentions)
Gapdh, by Qiagen (1 mentions)
Quantitect reverse transcription kit, by Qiagen (1 mentions)
Stat1, by Qiagen (1 mentions)
7500 fast real time pcr system, by Thermo Fisher Scientific (1 mentions)
Fast sybr green master mix, by Thermo Fisher Scientific (1 mentions)
Nanodrop nd 1000 spectrophotometer, by Avantor (1 mentions)
Bcl 2, by Qiagen (1 mentions)
Isg15, by Qiagen (1 mentions)
2 protocols using usp18
1
Spleen RNA Expression Analysis
2
Gene Expression Analysis by qRT-PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RNA isolation, purification, and reverse-transcription to cDNA were performed as instructed by the manufacturer (Qiagen, Hilden, Germany). Quantification of RNA was done with a NanoDrop ND-1000 spectrophotometer (Peqlab Biotechnologie GmbH, Erlangen, Germany). For quantitative real-time PCR (qRT-PCR), Fast SYBR Green Master Mix (Applied Biosystems, Darmstadt, Germany) was used on the 7500 Fast Real-Time PCR System (Applied Biosystems, Darmstadt, Germany). Gene expression analysis was performed with GAPH, Ifnα2, Ifnα4, Ifnα5, Ifnβ1, Usp18, Irf7, Isg15, Mx1, Bst2, Oas1, Irf1, and Bcl2 assays (Qiagen, Hilden, Germany). For analysis, the expression levels of all target genes were adjusted to GAPDH expression levels (ΔCt). Gene expression values were then calculated based on the delta-delta-Ct (ΔΔCt) method relative to the naive controls. Relative quantities (RQs) were determined using the following equation: RQ = 2−ΔΔCt.
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