Cinobufagin was purchased from Chenguang Biotechnology (Baoji, China). MTT and DMSO were purchased from Sigma‐Aldrich (St Louis, MO, USA). RPMI 1640, FBS, and antibiotics were purchased from Thermo Fisher Scientific (Boston, MA, USA). HUVEC was purchased from ATCC (Manassas, VI, America). Mice hemangioendothelioma cell EOMA was purchased from JNObio (Guangzhou, China). Hoechst staining kit, JC‐1 kit for mitochondrial membrane potential, reactive oxygen species (ROS) assay kit and ROS inhibitor N‐acetyl‐l ‐cysteine (NAC) were purchased from Beyotime Biotechnology (Shanghai, China). Annexin V‐FITC/7AAD apoptosis detection kit was purchased from Becton Dickinson (BD, Franklin Lakes, NJ, USA). Antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA) and Abcam (Cambridge, UK). Rapamycin was purchased from Meilunbio (Dalian, China). Endostatin was purchased from Simcere (Shandong, China).
Rapamycin
Manufactured by Meilun
Sourced in China
Rapamycin is a macrolide compound produced by the bacterium Streptomyces hygroscopicus. It functions as an immunosuppressant and has antiproliferative properties.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rapamycin, by Solarbio (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Hoechst staining kit, by Beyotime (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (1 mentions)
Annexin 5 fitc 7 aad apoptosis detection kit, by BD (1 mentions)
Ros inhibitor n acetyl l cysteine nac, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
X tremegene hp dna transfection reagent, by Roche (1 mentions)
Dulbecco modified eagle medium (dmem), by Meilun (1 mentions)
Everolimus, by Meilun (1 mentions)
Coumarin 6, by Merck Group (1 mentions)
Ir820, by Merck Group (1 mentions)
Bafilomycin a1, by Solarbio (1 mentions)
Rpmi 1640 medium, by Solarbio (1 mentions)
Thioflavin t, by Merck Group (1 mentions)
4 chlorobenzenesulfonate salt did, by Biotium (1 mentions)
12 protocols using rapamycin
1
Cinobufagin's Anti-Angiogenic Effects
2
Cell Culture and Transfection Protocol
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Cells were cultured in DMEM (Meilun Biotechnology, China) containing 1× antibiotics (containing Penicillin-Streptomycin-Amphotericin B) and 10% FBS (Biological Industries, Israel) and saved with CELLSAVING (New Cell & Molecular Biotech). Glutamine-deprived and arginine-deprived DMEM was obtained from NJJCBIO (China) and Meilun Biotechnology (China). Plasmids were transfected into cells with EZ Trans (Life-iLab, China) and X-tremeGENE HP DNA transfection reagent (Roche) following the manuals. Rapamycin, temsirolimus and everolimus for cell viability assay were purchased from Meilun Biotechnology, China.
3
Rapamycin-Based Nanoparticle Formulation
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Rapamycin was purchased from Dalian Meilun Biological Technology Co., Ltd. (Dalian, China). IR820, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), polyoxide castor oil, and coumarin 6 were supplied from Sigma-Aldrich (Shanghai, China). Dimethyl sulfoxide (DMSO), Tween 80 (medical-grade), and curcumin were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). RPMI 1640 and trypsin were purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Life Technologies GmbH (Darmstadt, Germany). Saline was purchased from Sichuan Kelun Pharmaceutical Co., Ltd. (Chengdu, China).
4
Rapamycin and Bafilomycin A1 in PTC Cells
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The human PTC cell line TPC-1 was kindly provided by Dr Ye Lei (Shanghai Rui Jin Hospital, Shanghai, China) and the normal human thyroid follicular epithelial cell line Nthy-ori3-1 was purchased from Shanghai Cell Biochemical Institute (Shanghai, China). All the cell lines were cultured in RPMI-1640 medium (Solarbio, Beijing, China) with 10% fetal bovine serum (FBS) (Gemini, USA) and incubated in a humidified atmosphere of 5% CO2 at 37°C. The following chemicals were used: rapamycin (Dalian Meilun Biotechnology: XB13514, 10nM) and bafilomycin A1 (Solarbio: A8510, 5µM and 10µM).
5
Theranostic Nanoparticles for Alzheimer's
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Inositol was purchased from Shanghai Yi En Chemical Technology Co., Ltd. (Shanghai, China). Oxalyl chloride and Thioflavin-T was obtained from Sigma–Aldrich Co., LLC (USA). DSPE-PEG2000-Mal and DSPE-PEG5000-Mal were purchased from Ponsure Biotechnology (Shanghai, China). DSPE-hyd-DSPE5000-Mal was obtained from Jenkem Technology Co., Ltd. (Beijing, China). PLGA (MW = 3000) was gained from Trueturn-Biological technology Co., Ltd. (Beijing, China). The KLVFFC and C-Acp-Cyclo (CDAGRKQKC) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). Aβ1–42 and Aβ42-FITC were obtained from GL Biochem Ltd. (Shanghai, China). Rapamycin was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). 4-Chlorobenzenesulfonate salt (DiD) was gained from Biotium (Hayward, CA, USA). The anti-TAU (phospho S396) and anti-beta amyloid1–42 were purchased from Abcam Ltd. (Cambridge, UK), and the APP polyclonal antibody and phosphorylated TAU antibody [p-TAU (Ser 404)] were purchased from Abclonal (China). ELISA kits (IL-10, IFN-γ and TNF-α) was obtained from BD Pharmingen (USA). Tissue ROS test kit were purchased from BestBio Science (Nanjing, China). The chemical used in this study were analytical grade.
6
Notoginsenoside R1 Autophagy Regulation
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Notoginsenoside R1 (purity > 98%), rapamycin, and 3-methyladenine (3-MA) were bought from Dalian Meilun Biotechnology Co. Ltd. (Dalian, Liaoning, China). Selective MTR1A inhibitor was provided by Master of Small Molecules (MCE, Cat No: S26131). Antibodies against beclin-1 (A7353), LC3B (A19665), phosphoinositide 3-kinase (phospho-PI3K, AP0854), phospho-Akt (AP0098), PI3K p85 (A11402), Akt (A18120), Bax (A19684), cleaved caspase-3 (A19654), p62 (A11250), mTOR (A2445), MTNR1A (A13030), and β-actin (AC026) were purchased from ABclonal Biotechnology Co. Ltd. (Wuhan, Hubei, China). Bcl-2 antibody (abs131701) was bought from Apicent Biological Technology Co. Ltd. (Shanghai, China). p-mTOR antibody (5536S) was obtained from Cell Signaling Technology (Danvers, MA, United States).
7
Proline and Rapamycin in Nude Mice Xenograft
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The animal experiments were performed under standard conditions. Twenty-five nude mice (male, 4-6 weeks old) were used and purchased from the Animal Center at Jiangsu University. The mice were randomly divided into five groups with five mice in each group. At the same time, all of the mice were injected subcutaneously with 1×105 cells transfected sh-EGFP plasmid, sh-ALKBH5 plasmid, or PYCR2-siRNA. We randomly chose two groups from three PYCR2-siRNA groups, when the average tumor volume was about 50 mm3, they were injected with a proline solution (5mm, 100µL) in the abdominal cavity, one of them also injected with 4mg/kg Rapamycin (Meilun, Dalian, China). Tumor growth was monitored every two days.
8
Investigating JNK3 and mTOR Signaling
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Rotenone (Nanjing Xinfan Biotechnology Co., Ltd., Shanghai, China), sunflower oil (Shanghai Lisheng Chemicals Co., Ltd., Shanghai, China), LY294002 (Cell Signaling Technology, Inc., Danvers, MA, USA), rapamycin (Dalian Meilun Biotechnology Co., Ltd., Dalian, China), rabbit anti-rat JNK3/p-mTOR/β-actin monoclonal antibody and goat anti-rabbit IgG secondary antibody (cat. nos. E-AB-60615, E-AB-63517, E-AB-30419 and E-AB-1003, respectively; Wuhan Elabscience Biotechnology Co., Ltd., Wuhan, China), TRIzol (Genenode, Beijing, China), RIPA lysate (Shanghai Huzhen Biological Technology Co., Ltd., Shanghai, China), PMSF (Nanjing Shengxing Biotechnology Co., Ltd., Nanjing, China), BCA protein kit (Shanghai Beiyi Bioequip Information Co., Ltd., Shanghai, China), reverse transcription kit and fluorescence quantitative PCR kit (both from Guangzhou Huijun Biotechnology Co., Ltd., Guangzhou, China). Primers were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China).
9
Rapamycin and 5-FU Synergistic Effects on Colorectal Cancer
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Rapamycin and 5-FU were obtained from meilunbio (Dalian, China). The anti Caspase3 antibody, Cleaved Caspase3 antibody, p-AKT (ser 473) antibody, p-AKT (Thr 308) antibody, p70 S6 Kinase antibody, and p-p70S6 Kinase (Thr 389) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). The ki-67 antibody was purchased from Abcam (Cambridge, UK). The BCL-2 antibody, Bax antibody, AKT antibody, p53 antibody, E-Cadherin antibody, N-Cadherin antibody, Vimentin antibody, MMP-9 antibody and PCNA antibody were purchased from Proteintech (Wuhan, China). The GAPDH rabbit polyclonal antibody was obtained from XianZhi Biotech (Hangzhou, China). The peroxidase-conjugated secondary antibodies were obtained from CWBIO (Taizhou, China).
The human CRC cell line HCT-116 and SW-480 cells were purchased from iCell Bioscience Inc (Shanghai, China). The cells were cultured in a DMEM medium containing 10% FBS in a 37 °C incubator containing 5% CO2.
Female athymic nude mice (6–8 weeks old) were purchased from Guangdong Medical Laboratory Animal Center. All animal studies were performed in accordance with the Guide for the Care and Use of Laboratory Animals. The Laboratory Animal Ethics Committee of the Third Affiliated Hospital of Guangzhou Medical University approved all experimental protocols.
The human CRC cell line HCT-116 and SW-480 cells were purchased from iCell Bioscience Inc (Shanghai, China). The cells were cultured in a DMEM medium containing 10% FBS in a 37 °C incubator containing 5% CO2.
Female athymic nude mice (6–8 weeks old) were purchased from Guangdong Medical Laboratory Animal Center. All animal studies were performed in accordance with the Guide for the Care and Use of Laboratory Animals. The Laboratory Animal Ethics Committee of the Third Affiliated Hospital of Guangzhou Medical University approved all experimental protocols.
10
Rapamycin Radiation Protection Protocol
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Rapamycin (> 98% purity) was purchased from Dalian Meilun Biotechnology Co. Ltd (Cas:53123-88-9; China). Rapamycin was dissolved in ethanol at 10 mg/ml and diluted in 5% Tween-80 and 5% polyethylene glycol 400 (Solarbio Science & Technology Co., Ltd, China). For Rapamycin treatment, 6 h after irradiation, mice were treated with Rapamycin (4 mg/kg) by subcutaneous (s.c.) injection and then the injection was repeated every other day. As a control, mice were irradiated or nonirradiated and received s.c. injection of the same volume of vehicle (200 μl).
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